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| Adje, F.; Lozano, Y.F.; Meudec, E.; Lozano, P.; Adima, A.; N'zi, G.A.; Gaydou, E.M.. |
| Following the development of new applications of pilot plant scale extraction and formulation processes for natural active bioproducts obtained from various under-utilized tropical plants and herbs, we have manufactured water-extracts from Delonix regia flowers, grown in Ivory Coast. These extracts, which contain polyphenols, are traditionally home made and used as healthy bioproducts. They are reddish-coloured due to the presence of anthocyanins. The three major anthocyanins in these extracts have been characterized. The molecular structures were confirmed by LC-SM analysis. Amongst them, two are described for the first time in Delonix regia |
| Tipo: Journal Article |
Palavras-chave: DELONIX REGIA; POINCIANA REGIA; ANTHOCYANIN; POLYPHENOL; EXTRACT; BIODIVERSITY. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101b7d9f8f&uri=/notices/prodinra1/2010/09/ |
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| Fulcrand, H.; Mane, C.; Preys, S.; Mazerolles, G.; Bouchut, C.; Mazauric, J.P.; Souquet, J.M.; Meudec, E.; Li, Y.; Cole, R.B.; Cheynier, V.. |
| Lower molecular weight polyphenols including proanthocyanidin oligomers can be analyzed after HPLC separation on either reversed-phase or normal phase columns. However, these techniques are time consuming and can have poor resolution as polymer chain length and structural diversity increase. The detection of higher molecular weight compounds, as well as the determination of molecular weight distributions, remain major challenges in polyphenol analysis. Approaches based on direct mass spectrometry (MS) analysis that are proposed to help overcome these problems are reviewed. Thus, direct flow injection electrospray ionization mass spectrometry analysis can be used to establish polyphenol fingerprints of complex extracts such as in wine. This technique... |
| Tipo: Journal Article |
Palavras-chave: MASS SPECTROMETRY; PHENOLIC COMPOUND; PROANTHOCYANIDIN. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200927660e8f&uri=/notices/prodinra1/2010/08/ |
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| Ducasse, M.A.; Williams, P.; Meudec, E.; Cheynier, V.; Doco, T.. |
| The oligosaccharides of Carignan and Merlot wines have been characterized for the first time. In a first step, this fraction was prepared after discoloration of the wines and was collected by elution on an HPSEC system. In a second step, the glycosyl composition and linkages of wine oligosaccharides were determined by several methods. High resolution MS spectra of the Carignan and Merlot oligosaccharide fractions were obtained on an AccuTOF mass spectrometer equipped with an electrospray ionization (ESI) source and a time-of-flight (TOF) mass analyser.Oligosaccharides were present at a concentration of 330 and 250 mg/L in Carignan and Merlot red wines, respectively. Glycosyl residue composition analysis showed the presence of mannose, arabinose, galactose,... |
| Tipo: Journal Article |
Palavras-chave: WINE; OLIGOSACCHARIDE; CARIGNAN; MERLOT; ESI-TOF; GLUCURONOXYLAN; OLIGOGALACTURONIC ACID; OLIGORHAMNOGALACTURONIC ACID. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010d685164d&uri=/notices/prodinra1/2010/08/ |
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| Bach, B.; Meudec, E.; Lepoutre, J.P.; Rossignol, T.; Blondin, B.; Dequin, S.; Camarasa, C.. |
| The gamma-aminobutyrate (GABA) shunt, an alternative route for the conversion of alpha-ketoglutarate to succinate, involves the glutamate decarboxylase Gad1p, the GABA transaminase Uga1p and the succinate semialdehyde dehydrogenase Uga2p. This pathway has been extensively described in plants and animals, but its function in yeast remains unclear. We show that the flux through Gad1p is insignificant during fermentation in rich sugar-containing medium, excluding a role for this pathway in redox homeostasis under anaerobic conditions or sugar stress. However, we found that up to 4 g of exogenous GABA/liter was efficiently consumed by yeast. We studied the fate of this consumed GABA. Most was converted into succinate, with a reaction yield of 0.7 mol/mol. We... |
| Tipo: Journal Article |
Palavras-chave: SUCCINATE; PHYSIOLOGIE INTEGRATIVE; METABOLISME CENTRAL. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20106df112ac&uri=/notices/prodinra1/2010/08/ |
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| Aguié-Beghin, V.; Sausse, P.; Meudec, E.; Cheynier, V.; Douillard, R.. |
| The interactions between proteins and plant polyphenols are responsible for astringency and haze formation in beverages and may participate in foam stabilization. The effect of phenolic compounds with different structures, namely, catechin (C), epicatechin (Ec), epigallocatechin (Egc), epicatechin gallate (EcG), and epigallocatechin gallate (EgcG), on the surface properties at the air/liquid interface of P-casein, chosen as model protein, were monitored by tensiometry and ellipsometry. The formation of complexes in the bulk phase was measured by electrospray ionization mass spectrometry (ESI-MS). Adsorption of polyphenols from pure solution was not observed. Surface pressure, surface concentration, and dilational modulus of the protein adsorption layer... |
| Tipo: Journal Article |
Palavras-chave: ADSORPTION KINETIC; ADSORPTION LAYER; BETA-CASEIN; POLYPHENOL; PROTEIN POLYPHENOL INTERACTION; SPECTROSCOPIC ELLIPSOMETRY; DROP TENSIOMETER; ELECTROSPRAY IONIZATION SPECTROMETRY (ESI-MS); AIR/LIQUID INTERFACE. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20098a7ddb68&uri=/notices/prodinra1/2010/08/ |
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