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Registros recuperados: 27
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A direct monoclonal antibody sandwich immunoassay for detection of Bonamia ostreae (Ascetospora) in hemolymph samples of the flat oyster Ostrea edulis (Mollusca : Bivalvia) ArchiMer
Cochennec, Nathalie; Hervio, Dominique; Panatier, B; Boulo, Viviane; Mialhe, Eric; Rogier, H; Grizel, Henri; Paolucci, F.
Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming and relatively inefficient In the study of ongoing epizootics Alternatively, more effluent investigative tools are needed to accelerate disease recognition and quantification Such methods are needed to prevent control and eradicate molluscan diseases This paper reports the development of a direct sandwich enzyme-lmmunosorbent assay (ELISA) for the ascetosporan intrahemocytic parasite Bonamiao ostreae of the flat oyster Ostrea edulis. Bonamiasis is a commercially important epizootic disease of oysters A Bonamia-specific monoclonal antibody is employed in the...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/publication-2765.pdf
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An infection of Ruditapes decussatus (Bivalvia) by Rickettsia ArchiMer
Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Comps, Michel; Ruano, Francisco; Grizel, Henri.
The authors report the presence of a Rickettsia in Ruditapes decussatus from natural populations of the Algarve (Portugal). Light microscopic shows infections of varying importance in gill tissues. Some enclose small procaryotic colonies (cp) scattered in all lamellae; in others, the colonies are scarce and hypertrophic (100 mu m). These different pictures suggest an amplification process. Digestive diverticulae may also be infected, but to a lesser extent. The ultrastructural features of this intravacuolar (mvd: densified vacuolar membrane) procaryote (P) and its multiplication by scission without a complex developmental cycle allows its classification in the order Rickettsiales.
Tipo: Text Palavras-chave: Ruditapes philippinarum; Pathogens; Histopathology; Pathology.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-3107.pdf
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Bivale mollusc cell culture ArchiMer
Mialhe, Eric; Boulo, Viviane; Grizel, Henri.
The historical background of bivalve cell culture and its potential uses in molluscan pathology are reviewed. Then, primary cultures for the study of some host-pathogen interactions at the cellular level are considered. Next, alternative methods to classical cell cultures are examined that may lead to the production of bivalve celllines.
Tipo: Text Palavras-chave: Cell culture; Pathology; Molluscs.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3122.pdf
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Bonamiasis : a model study of diseases in marine molluscs ArchiMer
Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne.
Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!.
Tipo: Text Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf
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Branchial Rickettsiales-like infection associated with a mass mortality of sea scallop Pecten maximus ArchiMer
Legall, Ghyslaine; Chagot, Dominique; Mialhe, Eric; Grizel, Henri.
A branchial infection of Pecten maximus is histologically and ultrastructurally described. Gill epithelial cells were heavily infected with colonies of procaryotes, believed ta belong to the Rickettsiales.
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Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3125.pdf
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Chemiluminescence analysis of the activity of Pecten maximus hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms ArchiMer
Le Gall, G; Bachere, Evelyne; Mialhe, Eric.
During a study of Rickettsiales-like organisms (RLO) in Pecten maximus gill, histological observation of free RLO in hemoclymph led us to consider interactions between the pathogen and the hemocytes. Firstly, the production of oxygen radicals by hemocytes was established using a luminol-enhanced chemiluminescence method with zymosan axs stimulant. The effects of serveral inhibitors of oxidative metabolism were investigated. The internalization of RLO into hemocytes was demonstrated by electron microscopy but was not related to chemiluminescent activity. The possible involvement of RLO acid phosphatase as the inhibitor of respiratory burst was assessed.
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2759.pdf
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Epizootiological study of rickettsiosis of the Saint-Jacques scallop Pecten maximus ArchiMer
Le Gall, G; Mialhe, Eric; Chagot, D; Grizel, Henri.
Rickettsia-like microorganisms were described in gill endothelial cells during mass mortalities of Saint-Jacques scallops Pecten maximus in Saint-Brieuc Bay (North Brittany, France) during winter 1985-86. In order to evaluate the epizootiological significance of rickettsiosis of scallops, an analytical and descriptive epizootiological study was undertaken. The study included the geographic distribution, incidence and severity of the disease for several northern European and French scallop populations. Since the center of French scallop production is in Saint-Brieuc Bay, epizootiological investigations were concentrated in this area. Dissemination appeared to be by horizontal transmission. The pathogenic significance of the rickettsia-like organism in...
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2758.pdf
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Genomic DNA cloning of rickettsia-like organisms (RLO) of Saint-Jacques scallop Pecten maximus : evaluation of prokaryote diagnosis by hybridization with a non-isotopically labelled probe and by polymerase chain reaction ArchiMer
Kellner-cousin, Kristell; Le Gall, G; Despres, Béatrice; Kaghad, M; Legoux, P; Shire, D; Mialhe, Eric.
Genomic DNA fragments of a rickettsia-like organism (RLO) of Saint-Jacques scallop, Pecten maximus, were extracted from purified RLO and cloned. A DNA sequence of 1500 bp taken from one of the sequenced clones was coupled to horseradish peroxidase and the resulting probe was assayed for the diagnosis of RLO by nucleic acid hybridization. The specificity of the probe for RLO DNA was established from its lack of hybridization with scallop DNA. The sensitivity limit of the probe detected by enhanced chemiluminescence was determined to be around 500 ng of total RLO nucleic acids, equivalent to about 2.5 X 106 copies of RLO DNA. A pair of polymerase chain reaction (PCR) primers were synthesized that amplified a 1300 bp segment of the 1500 bp RLO fragment. After...
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Ano: 1993 URL: http://archimer.ifremer.fr/doc/1993/publication-2771.pdf
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In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens ArchiMer
Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric.
The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few...
Tipo: Text Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF
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Infectious pathology in mollusc and shrimp hatcheries ArchiMer
Mialhe, Eric.
Development of aquaculture has been linked to zootechnical mastering of larval production in hatcheries. Regular and excessive use of antibiotics (antifungal or antibacterial) may thus also be reduced, permitting to limit release of these products in sea-water and, consequently, avoiding progressive selection of resistant-strains.
Tipo: Text Palavras-chave: Viruses; Infectious diseases; Epidemiology; Pathogens; Bacterial diseases; Pathology; Larval development; Mollusc culture; Shrimp culture.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1487.pdf
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Isolement et purification de Marteilia (Protozoa : Ascetospora) parasites de bivalves marins ArchiMer
Mialhe, Eric; Bachere, Evelyne; Le Bec, Claude; Grizel, Henri.
Marteilia (Protozoa: Ascetospora) parasites of Ostrea edulis and Mytilus edulis, have been isolated and purified by an original method. The electron microscopie study of the purified pansporoblasts and spores confirms the integrity of the parasite and shows some new characteristics.
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Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/publication-3223.pdf
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Localisation and characterization of acid phosphate activity in Bonamia ostreae (Ascetospora), an intrahemocytic protozoan parasite of the flat oyster Ostrea edulis (Bivalvia) ArchiMer
Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric.
Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed.
Tipo: Text Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf
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Localization and characterization of acid phosphatase activity in Bonamia ostreae (Ascetospora), an intrahemocytic protozoan parasite of the fIat oyster Ostrea edulis (Bivalvia) ArchiMer
Hervio, Dominique; Chagot, D; Godin, P; Grizel, Henri; Mialhe, Eric.
Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the substrate (cytidine 5' monophosphate). Lead phosphate precipitation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as 'dense bodies'. Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostreae is discussed.
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/00182/29311/28273.pdf
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Luminol-dependent chemiluminescence by hemocytes of two marine bivalves, Ostrea edulis and Crassostrea gigas ArchiMer
Bachere, Evelyne; Hervio, Dominique; Mialhe, Eric.
Microbicidal phagocytic function and oxidative metabolism of the hemocytes of 2 oyster species, Ostrea edulis and Crassostrea gigas, were investigated using a luminol-enhanced chemiluminescence (technique. First, experimental parameters adapted to marine bivalve hemocytes were established on hemolymph pools of C. gigas in order to obtain qualitatively and quantitatively homogeneous samples and so to perform statistically comparable assays. The use of Modified Alsever Solution (allowed hemocytes to be kept non-aggregated and in a non-stimulated state until starting the assays. A number of aliquots of 2 X 10(5) hemocytes with an MAS final concentration of 3.5 % showed high chemiluminescent response after stimulation by zymosan particles A particle:hemocyte...
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2761.pdf
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Monoclonal antibodies : a tool for molluscan pathology ArchiMer
Mialhe, Eric; Boulo, Viviane; Grizel, Henri; Rogier, Hervé; Paolucci, Francis.
Hybridoma technology is reviewed, and the characteristics of monoclonal antibodies are compared with those of polyclonal antibodies. The contribution of monoclonal antibodies to molluscan pathology is developed with special emphasis on their use as diagnostic tools. The results of studies with monoclonal antibodies prepared against the protozoan oyster pathogen Bonamia ostreae are briefly described.
Tipo: Text Palavras-chave: Pathology; Molluscs; Monoclonal antibody technology.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3121.pdf
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Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia) ArchiMer
Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis.
The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These...
Tipo: Text Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf
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Monoclonal antibodies against sporangia and spores of Marteilia sp. (Protozoa: Ascetospora) ArchiMer
Robledo, Jaf; Boulo, Viviane; Mialhe, Eric; Despres, Béatrice; Figueras, Antonio.
Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used purification protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1). Antibodies cross-reacted with Marteilia refringens from...
Tipo: Text Palavras-chave: Marteilia sp.; Mytilus edulis; Monoclonal antibodies.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00196/30730/29404.pdf
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Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maximus (Bivalvia): application to indirect immunofluorescence diagnosis ArchiMer
Le Gall, Guillaume; Mourton, Chantal; Boulo, Viviane; Paolucci, Francis; Pau, B; Mialhe, Eric.
Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maximus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis.
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/00190/30135/28778.pdf
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Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis ArchiMer
Le Gall, Gyslaine; Mourton, Chantal; Boulo, Viviane; Paolucci, Francis; Pau, Bernard; Mialhe, Eric.
Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maxiumus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis
Tipo: Text Palavras-chave: Diagnosis; Rickettsiales like organism; Monoclona antibody; Pecten maximus.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/publication-2762.pdf
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Pathologie des invertébrés marins d'intérêt aquacole. Contribution et stratégies de recherches ArchiMer
Mialhe, Eric.
Les productions d'invertébrés sont prépondérantes en aquaculture marine qui est économiquement très importate pour un grand nombre de pays, notamment de la zone Sud. Cette activité est confrontée à de nombreux problèmes des pathologie qui remettent en cause sa périnnité et son développment. Jusque dans les années quatre-vingt, les travaux relatifs à la pathologie des mollusques et des crevettes ont essentiellement porté sur la description des agents pathogènes. La création en 1985 par l'ISTPM/IFREMER d'un laboratoire pourvu de tous les équipements nécessaires aux techniques de pathologie a permis de mettre en place un programme de recherches orienté vers le contrôle des maladies. Une double stratégie de travail a été alors défine pour apporter  à...
Tipo: Text Palavras-chave: Pathologie; Immmunologie; Génétique; Mollusque; Crevette; Diagnostic; Epidémiologie; Traitement; Sélection; Transformation génétique.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00443/55479/57009.pdf
Registros recuperados: 27
Primeira ... 12 ... Última
 

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