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Pectinase-hyperproducing mutants of Aspergillus niger C28B25 for solid-state fermentation of coffee pulp IRD
Antier, Philip; Minjares, A.; Roussos, Sevastianos; Raimbault, Maurice; Viniegra-Gonzalez, G..
The aim of this study was to improve mold strains for the production of pectinases by solid-state fermentation (SSF) of coffee pulp. A screening of 248 strains, isolated in Mexico's coffee-growing areas, permitted us to select a wild strain of #Aspergillus niger$ which in 72 h attains a peak production of 27.7 U ml-1(138 U g-1 dry pulp) of pectinase measured by viscosimetry. Though the use of a selective culture medium with low water activity (aw = 0.954) with 2-deoxy-glucose (2DG) it was possible to isolate pectinase-hyperproductive mutant strains for SSF(44.5 U ml-1, 228 U g-1 dry pulp). Derepressed mutant strains that hyperproduced pectinase by submerged fermentation (SmF) were also obtained using a classic selective medium with high water activity...
Tipo: Text Palavras-chave: FERMENTATION EN MILIEU SOLIDE; PULPE; CAFE; ENZYME; CHAMPIGNON; ETUDE COMPARATIVE; ISOLEMENT; SOUCHE; MILIEU DE CULTURE; ACTIVITE ENZYMATIQUE; MUTANT; PECTINASE; CARACTERISATION.
Ano: 1993 URL: http://www.documentation.ird.fr/hor/fdi:39182
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