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A histological examination of grafting success in pearl oyster Pinctada margaritifera in French Polynesia ArchiMer
Cochennec-laureau, Nathalie; Montagnani, Caroline; Saulnier, Denis; Fougerouse, Angelique; Levy, Peva; Lo, Cedrik.
Pearl oyster grafting is a complex surgical operation that should lead to pearl formation after approximately eighteen months. Although this technique has been used for many years in French Polynesia, the grafting process is still not standardised. While studies have been carried out in order to improve graft performance and yield, these remain highly variable due to post-grafting mortality, nucleus rejection and unreliable pearl quality, all of which constrain pearl farm profitability. The present study uses histological analysis to monitor oysters that either rejected or retained their nuclei. Both groups of oysters are compared in terms of evolution of the graft, which could influence retention, and the development of a pearl sac in cases where grafting...
Tipo: Text Palavras-chave: Grafting process; Pearl formation; Pearl oyster; Pteriidae; Pinctada margaritifera.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11288/7823.pdf
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Antiviral immunity in marine molluscs ArchiMer
Green, Timothy J.; Raftos, David; Speck, Peter; Montagnani, Caroline.
Marine molluscs, like all living organisms, are constantly exposed to viruses and have evolved efficient antiviral defences. We review here recent developments in molluscan antiviral immunity against viruses belonging to the order Herpesvirales. Emerging results suggest an interferon-like response and autophagy are involved in the antiviral defence of bivalves to viral infection. Multifunctional plasma proteins from gastropods and bivalves have been identified to have broadspectrum antiviral activity against mammalian viruses. The antiviral defences present in molluscs can be enhanced by genetic selection, as shown by the presence of oyster strains specifically resistant to ostreid herpesvirus type 1. Whether varying amounts or different isofornns of these...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00296/40743/39736.pdf
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Cg-TIMP, un inhibiteur de metalloproteinase isolé chez Crassostrea gigas : étude de son rôle dans la réparation des lésions et dans les mécanismes de défense ArchiMer
Escoubas, Jean-michel; Montagnani, Caroline; Le Roux, Frédérique; De Lorgeril, Julien; Berthe, Franck; Bachere, Evelyne.
We isolated complementary DNA (Cg-TIMP) from Crassostrea gigas that codes for a protein highly homologous to vertebrate tissue inhibitors of metalloproteinases (TIMPs). In vertebrates, TIMPs are multifunctional proteins that play a key role in extracellular matrix metabolism. They are involved in physiological processes (e.g. embryonic development, damage repair, angiogenesis) and pathological processes (e.g. inflammatory reactions, cancer).
Tipo: Text Palavras-chave: Proteine; Cg TIMP; ADN; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3268.pdf
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Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia ArchiMer
Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis.
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes...
Tipo: Text Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf
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Characterization of MRNP34, a novel methionine-rich nacre protein from the pearl oysters ArchiMer
Marie, Benjamin; Joubert, Caroline; Belliard, Corinne; Tayale, Alexandre; Zanella-cleon, Isabelle; Marin, Frederic; Gueguen, Yannick; Montagnani, Caroline.
Nacre of the Pinctada pearl oyster shells is composed of 98% CaCO3 and 2% organic matrix. The relationship between the organic matrix and the mechanism of nacre formation currently constitutes the main focus regarding the biomineralization process. In this study, we isolated a new nacre matrix protein in P. margaritifera and P. maxima, we called Pmarg- and Pmax-MRNP34 (methionine-rich nacre protein). MRNP34 is a secreted hydrophobic protein, which is remarkably rich in methionine, and which is specifically localised in mineralizing the epithelium cells of the mantle and in the nacre matrix. The structure of this protein is drastically different from those of the other nacre proteins already described. This unusual methionine-rich protein is a new member in...
Tipo: Text Palavras-chave: Matrix protein; Methionine-rich; Biomineralization; Mollusc; Nacre; Calcifying mantle.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00077/18870/16645.pdf
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Development of in situ hybridisation using 16S rRNA gene to monitor black-lip pearl oyster, Pinctada margaritifera, larvae in plankton samples ArchiMer
Thomas, Yoann; Belliard, Corinne; Garen, Pierre; Gueguen, Yannick; Montagnani, Caroline.
In French Polynesia, the black-lip pearl oyster Pinctada margaritifera has been farmed to produce pearls since the 1980s, forming the basis of a major industry. The sustainability of this activity relies on spat collection in the lagoons. However, pearl oyster spat can be difficult to identify for the evaluation of stock variations. It is especially hard to distinguish Pinctada spp. larvae at a very early stage of development. In the present study, a whole- mount in situ hybridisation (ISH) technique was developed to allow the discrimination of larvae of closely-related pearl oyster species found in the French Polynesian atolls. Using specific ribosomal 16S-DNA sequence data, we were able to successfully differentiate between Pinctada margaritifera and...
Tipo: Text Palavras-chave: Whole larvae in situ hybridisation; 16S rRNA; Plankton; Species identification; Pearl oyster; Pinctada margaritifera.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00035/14603/11923.pdf
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Different secretory repertoires control the biomineralization processes of prism and nacre deposition of the pearl oyster shell ArchiMer
Marie, Benjamin; Joubert, Caroline; Tayale, Alexandre; Zanella-cleon, Isabelle; Belliard, Corinne; Piquemal, David; Cochennec-laureau, Nathalie; Marin, Frederic; Gueguen, Yannick; Montagnani, Caroline.
Mollusca evolutionary success can be attributed partly to their efficiency to sustain and protect their soft body with an external biomineralized structure, the shell. Current knowledge of the protein set responsible for the formation of the shell microstructural polymorphism and unique properties remains largely patchy. In Pinctada margaritifera and Pinctada maxima, we identified 80 shell matrix proteins, among which 66 are entirely unique. This is the only description of the whole "biomineralization toolkit" of the matrices that, at least in part, is thought to regulate the formation of the prismatic and nacreous shell layers in the pearl oysters. We unambiguously demonstrate that prisms and nacre are assembled from very different protein repertoires....
Tipo: Text Palavras-chave: Mantle; Mollusk shell matrix proteins; Proteome; Transcriptome; Evolution.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00118/22959/20922.pdf
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Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections ArchiMer
Green, Timothy J.; Vergnes, Agnes; Montagnani, Caroline; De Lorgeril, Julien.
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102...
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Ano: 2016 URL: http://archimer.ifremer.fr/doc/00348/45875/45575.pdf
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Evidence of donor effect on cultured pearl quality from a duplicated grafting experiment on Pinctada margaritifera using wild donors ArchiMer
Tayale, Alexandre; Gueguen, Yannick; Treguier, Cathy; Le Grand, Jacqueline; Cochennec-laureau, Nathalie; Montagnani, Caroline; Ky, Chin-long.
Producing high quality cultured black pearls from Pinctada margaritifera is one of the major challenges for the "pearl oyster" industry in French Polynesia. In order to assess donor effect on cultured pearl quality, wild Pinctada margaritifera originating from the Tuamotu Archipelago were used in a duplicated grafting experiment. After 12 months of culture, nucleus retention was assessed and seven pearl quality traits recorded on the 454 cultured pearls harvested from the experiment. The traits scored were nacre thickness and pearl weight, surface defects, lustre, grade, and the colour components: 1) darkness of cultured pearl colour, and 2) visual perception of colour class (bodycolor and/or overtone). Our results demonstrate for the first time that...
Tipo: Text Palavras-chave: Cultured pearl oyster; Pearl quality; Nacre; Donor mollusc; Genetic selection; Pteriidae; Blacklip pearl oyster; Pinctada margaritifera; French Polynesia.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00116/22757/20541.pdf
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First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway ArchiMer
Montagnani, Caroline; Avarre, Jean-christophe; De Lorgeril, Julien; Quiquand, M; Boulo, Viviane; Escoubas, Jean-michel.
In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and...
Tipo: Text Palavras-chave: Damage associated molecular pattern; Tissue inhibitor of metalloproteinase; Metalloproteinase; Mollusk immunity; Crassostrea gigas; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2094.pdf
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Fondamentaux d’un développement durable du « système mer » Biotechnologies, bioingénieries marines et restauration écologique des milieux. L’immunobiologie pour lutter contre la mortalité des huîtres en élevage ArchiMer
Montagnani, Caroline.
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59826/62969.pdf
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Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters ArchiMer
De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume.
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf
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Influence of OSHV-1 oyster mortality episode on dissolved inorganic fluxes: An ex situ experiment at the individual scale ArchiMer
Richard, Marion; Bourreau, Juliette; Montagnani, Caroline; Ouisse, Vincent; Le Gall, Patrik; Fortune, Martine; Munaron, Dominique; Messiaen, Gregory; Callier, Myriam; Roque D'Orbcastel, Emmanuelle.
Ostreid herpesvirus 1 (OsHV-1 μvar) infection has caused significant mortalities in juvenile oysters (Crassostrea gigas). In contrast to the practices of other animal production industries, sick and dead oysters are not separated from live ones and are left to decay in the surrounding environment, with unknown consequences on fluxes of dissolved materials. A laboratory approach was used in this study to test the influence of oyster mortality episode on dissolved inorganic fluxes at the oyster interface, dissociating (i) the effect of viral infection on metabolism of juvenile oysters and (ii) the effect of flesh decomposition on oxygen consumption and nutrient releases at the individual scale. Nine batches of juvenile oysters (Individual Total wet weight...
Tipo: Text Palavras-chave: Crassostrea gigas; Mortality; Ostreid herpesvirus 1; Juvenile; Spat; Mineralisation; Oxygen consumption; Nutrient fluxes.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00378/48973/49380.pdf
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Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication ArchiMer
Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters...
Tipo: Text Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf
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Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas ArchiMer
Lafont, Maxime; Petton, Bruno; Vergnes, Agnes; Pauletto, Marianna; Segarra, Amelie; Gourbal, Benjamin; Montagnani, Caroline.
In the last decade, a paradigm shift has emerged in comparative immunology. Invertebrates can no longer be considered to be devoid of specific recognition and immune memory. However, we still lack a comprehensive view of these phenomena and their molecular mechanisms across phyla, especially in terms of duration, specificity, and efficiency in a natural context. In this study, we focused on a Lophotrochozoan/virus interaction, as antiviral priming is mostly overlooked in molluscs. Juvenile Crassostrea gigas oysters experience reoccurring mass mortalities events from Ostreid herpes virus 1 with no existing therapeutic treatment. Our results showed that various nucleic acid injections can prime oysters to trigger an antiviral state ultimately protecting them...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00407/51811/52413.pdf
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Molecular detection of betanodavirus from the farmed fish, Platax orbicularis (Forsskal) (Ephippidae), in French Polynesia ArchiMer
David, R.; Treguier, Cathy; Montagnani, Caroline; Belliard, Corinne; Levy, Peva; Nedelec, Georges; Joufoques, Vaiana; Remoissenet, G.; Gueguen, Yannick; Cochennec-laureau, Nathalie.
Tipo: Text Palavras-chave: Betanodavirus; Detection; French Polynesia; Genotype; Platax orbicularis.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11380/7972.pdf
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Mortalités d'huîtres creuses adultes (Crassostrea gigas) et infection à Vibrio aestuarianus - AESTU ArchiMer
Travers, Marie-agnes; Degremont, Lionel; De Lorgeril, Julien; Azema, Patrick; Montagnani, Caroline; Benabdelmouna, Abdellah; Nicolas, Jean-louis; Le Roux, Frederique.
En 2012, le nombre de cas de mortalités d’huîtres creuses adultes rapportés dans le cadre du réseau Repamo, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Afin d’apporter de premiers éléments de réponse concernant cette augmentation, les objectifs de cette étude étaient de déterminer si l’émergence/la ré-émergence de V. aestuarianus était associée (i) à l’apparition d’un génotype bactérien particulier et/ou (ii) à une sensibilité accrue de certains animaux. L’Ifremer dispose d’une collection de souches bactériennes appartenant majoritairement au genre Vibrio et qui comporte différents isolats de V. aestuarianus, collectés depuis 2001 pendant ou hors épisodes de mortalité. La comparaison de ces isolats en termes de...
Tipo: Text Palavras-chave: Vibrio aestuarianus; Huître creuse; Crassostrea gigas; Bactérie; Ré-émergenece; Virulence; Génome; Statut immunitaire; Génétique.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00229/34037/32430.pdf
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Ontogeny and water temperature influences the antiviral response of the Pacific oyster, Crassostrea gigas ArchiMer
Green, Timothy; Montagnani, Caroline; Benkendorff, Kirsten; Robinson, Nick; Speck, Peter.
Disease is caused by a complex interaction between the pathogen, environment, and the physiological status of the host. Determining how host ontogeny interacts with water temperature to influence the antiviral response of the Pacific oysters, Crassostrea gigas, is a major goal in understanding why juvenile Pacific oysters are dying during summer as a result of the global emergence of a new genotype of the Ostreid herpesvirus, termed OsHV-1 μvar. We measured the effect of temperature (12 vs 22 °C) on the antiviral response of adult and juvenile C. gigas injected with poly I:C. Poly I:C up-regulated the expression of numerous immune genes, including TLR, MyD88, IκB-1, Rel, IRF, MDA5, STING, SOC, PKR, Viperin and Mpeg1. At 22 °C, these immune genes showed...
Tipo: Text Palavras-chave: Crassostrea; OsHV-1; Water temperature; Antiviral response; Herpesvirus.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00162/27318/25674.pdf
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OsHV-1 countermeasures to the Pacific oyster’s anti-viral response ArchiMer
Green, Timothy J.; Rolland, Jean-luc; Vergnes, Agnes; Raftos, David; Montagnani, Caroline.
The host-pathogen interactions between the Pacific oyster (Crassostrea gigas) and Ostreid herpesvirus type 1 (OsHV-1) are poorly characterised. Herpesviruses are a group of large, DNA viruses that are known to encode gene products that subvert their host’s antiviral response. It is likely that OsHV-1 has also evolved similar strategies as its genome encodes genes with high homology to C. gigas inhibitors of apoptosis (IAPs) and an interferon-stimulated gene (termed CH25H). The first objective of this study was to simultaneously investigate the expression of C. gigas and OsHV-1 genes that share high sequence homology during an acute infection. Comparison of apoptosis-related genes revealed that components of the extrinsic apoptosis pathway (TNF) were...
Tipo: Text Palavras-chave: Crassostrea; Anti-viral response; Apoptosis; Interferon-like; Poly I:C.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00279/39048/37610.pdf
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Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) ArchiMer
Green, Timothy; Montagnani, Caroline.
In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection...
Tipo: Text Palavras-chave: Crassostrea gigas; OsHV-1; Poly I:C; Vibrio splendidus; Interferon-like response.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26293/24739.pdf
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