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Registros recuperados: 11
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Anthropization Effects on the Filamentous Fungal Community of the Brazilian Catimbau National Park Rev. Bras. Ciênc. Solo
Cruz,Roberta; Ramos,Sérgio Murilo Sousa; Fonseca,Julyanna Cordoville; Motta,Cristina Maria de Souza; Moreira,Keila Aparecida.
ABSTRACT The Caatinga biome features an exclusive endemic biodiversity, and is characterized by the presence of xerophytic, deciduous vegetation, high temperatures, and low rainfall. This important park has undergone anthropization, especially through extraction of firewood and timber and growing plants for raising goats. The objectives of this study were to compare the communities of filamentous fungi present in the preserved area and in the anthropized soil of the Catimbau National Park in Buíque, PE, Brazil, and to evaluate the impacts of anthropization on such communities. A total of 12 collections of soil samples were made, six in the preserved area and six in the anthropic area, and the physicochemical properties of the soil samples were analyzed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caatinga; Diversity; Aspergillus; Gongronella; Penicillium.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100419
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Antioxidant Activities of Chicken Egg White Hydrolysates Obtained by New Purified Protease of Aspergillus avenaceus URM 6706 BABT
Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Oliveira,João Tiago Correia; Medeiros,Erika Valente; Souza-Motta,Cristina Maria de; Moreira,Keila Aparecida.
Abstract Protein hydrolysates originating from egg white have already been reported to be bioactive and, among their biological activities, possess the antioxidant property that protects the body from early ageing and diseases linked to oxidation. Therefore, the objective of this work was to evaluate the antioxidant activity of hydrolysates obtained by the hydrolysis of egg white from hen poultry. The protease produced by Aspergillus avenaceus URM 6706 was purified and subsequently applied to hydrolysate the egg white, and the degree of hydrolysis was verified during the protease exposure time (4-24 h). The hydrolysis was intensified over time of exposure to the protease. It was possible to detect the antioxidant activities of eliminating the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Purification; ABTS; +; DPPH; Chelators.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100508
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Aspergillus niveus Blochwitz 4128URM: new source for inulinase production BABT
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de.
Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
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Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system BABT
Bezerra,Raquel Pedrosa; Borba,Fernanda Katharine Sousa Lins; Moreira,Keila Aparecida; Lima-Filho,José Luis; Porto,Ana Lúcia Figueiredo; Chaves,Adilson Castro.
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylases; Aqueous two-phase systems; Bacillus subtilis; Partition; Poly (ethylene glycol).
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003
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Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes BABT
Duarte,Ana Rodrigues; Duarte,Débora Maria Rodrigues; Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Lima-Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
The partial characterization and purification of milk clotting enzyme obtained from the (root latex) of Jacaratia corumbensis O. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. The ammonium sulphate precipitate showed five fractions (AS1- 0-20%; AS2 - 20-40%; AS3 - 40-60%; AS4 - 60-80%; AS5 - 80-100%) and among the fractions obtained, the 40-60% fraction (AS3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. This fraction when applied on Mono Q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. The optimal pH for the crude and partially purified extract was 6.5 and 7.0, respectively. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jacaratia corumbensis O. kuntze; Milk clotting enzyme; Characterization; Purification; Vegetable enzyme.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100001
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Partial characterization of an inulinase produced by Aspergillus japonicus URM5633 BABT
Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Porto,Tatiana Souza; Spier,Michele Rigon; Soccol,Carlos Ricardo; Porto,Ana Lúcia Figueiredo; Souza-Motta,Cristina Maria; Moreira,Keila Aparecida.
Enzymes obtained by fermentation processes offer a number of advantages and have been widely researched and used throughout the world. This study aimed to partially characterise an inulinase produced from palm and cassava peel. The enzyme was produced via the solid-state fermentation of Aspergillus japonicus URM5633. The optimal temperatures were 50ºC and 55ºC, and the optimal pH values were 5.2 and 3.4 for inulinase fermentatively produced from palm and cassava peel, respectively. The thermostability measurements for inulinase produced in palm showed that the relative activity remained below 100% until 30 minutes of stability for all temperatures, but reached 106.8% at a temperature of 50ºC after 60 minutes. Inulinase from the crude extract of cassava...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Characterisation; Solid-state fermentation; Aspergillus japonicus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500005
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PARTIAL CHARACTERIZATION OF PROTEASES FROM STREPTOMYCES CLAVULIGERUS USING AN INEXPENSIVE MEDIUM BJM
Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile; Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de.
The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010
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Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite Ciência Rural
Soares,Elisângela França; Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Gomes,José Erick Galindo; Herculano,Polyanna Nunes; Moreira,Keila Aparecida.
Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzimas coagulantes do leite; Atividade proteolítica; Coagulante vegetal; Fruta-pão.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149
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Potential of quixaba (Sideroxylon obtusifolium) latex as a milk-clotting agent Ciênc. Tecnol. Aliment.
Silva,Anna Carolina da; Nascimento,Talita Camila Evaristo da Silva; Silva,Sheylla Araújo da; Herculano,Polyanna Nunes; Moreira,Keila Aparecida.
There are several obstacles to the use of chymosin in cheese production. Consequently, plant proteases have been studied as possible rennet substitutes, but most of these enzymes are unsuitable for the manufacture of cheese. The aim of this study was to evaluate the potential of latex from Sideroxylon obtusifolium as a source of milk-clotting proteases and to partially characterize the enzyme. The enzyme extract showed high protease and coagulant activities, with an optimal pH of 8.0 and temperature of 55 °C. The enzyme was stable in wide ranges of temperature and pH. Its activity was not affected by any metal ions tested; but was inhibited by phenylmethanesulfonyl fluoride and pepstatin. For the coagulant activity, the optimal concentration of CaCl2 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteolytic activity; Plant proteases; Rennet.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300017
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Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography BABT
Moreira,Keila Aparecida; Diogo,Margarida; Prazeres,Duarte Miguel; Lima Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Hydrophobic interaction chromatography; Plasmid; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
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Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor BABT
Porto,Ana Lúcia Figueiredo; Sarubbo,Leonie Asfora; Moreira,Keila Aparecida; Melo,Homero José Farias de; Lima-Filho,José Luís; Campos-Takaki,Galba Maria; Tambourgi,Elias Basile.
A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Efficiency; Mass transfer coefficient; Perforated Rotating Disc contactor; Aqueous two-phase system; Ascorbic oxidoreductase.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019
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