Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 11
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins BJMBR
Rocha,H.A.O.; Franco,C.R.C.; Trindade,E.S.; Carvalho,L.C.M.; Veiga,S.S.; Leite,E.L.; Dietrich,C.P.; Nader,H.B..
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5))...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CHO cells; Extracellular matrix proteins; Fucan; Glycosaminoglycans; Cell adhesion; Brown algae.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500009
Imagem não selecionada

Imprime registro no formato completo
Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells BJMBR
Nader,H.B.; Pinhal,M.A.S.; Baú,E.C.; Castro,R.A.B.; Medeiros,G.F.; Chavante,S.F.; Leite,E.L.; Trindade,E.S.; Shinjo,S.K.; Rocha,H.A.O.; Tersariol,I.L.S.; Mendes,A.; Dietrich,C.P..
The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heparin; Anticlotting activity heparan sulfate; Antithrombotic activity low molecular weight heparins; Preparation and antithrombotic activity antithrombotic agents; Development for clinical use crustacean heparin-like compounds; Antithrombotic activity antithrombotic agents vascular endothelial cells.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600002
Imagem não selecionada

Imprime registro no formato completo
Effect of b-D-xylosides bearing different aglycones on the synthesis of proteoglycans during the cell cycle of endothelial cells in culture Anais da ABC (AABC)
Moreira,C.R.; Lopes,C.C.; Porcionatto,M.A.; Esko,J.D.; Dietrich,C.P.; Nader,H.B..
Tipo: Info:eu-repo/semantics/other
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000100021
Imagem não selecionada

Imprime registro no formato completo
Effect of photodynamic therapy on the extracellular matrix and associated components BJMBR
Pazos,M.d.C.; Nader,H.B..
In many countries, photodynamic therapy (PDT) has been recognized as a standard treatment for malignant conditions (for example, esophageal and lung cancers) and non-malignant ones such as age-related macular degeneration and actinic keratoses. The administration of a non-toxic photosensitizer, its selective retention in highly proliferating cells and the later activation of this molecule by light to form reactive oxygen species that cause cell death is the principle of PDT. Three important mechanisms are responsible for the PDT effectiveness: a) direct tumor cell kill; b) damage of the tumor vasculature; c) post-treatment immunological response associated with the leukocyte stimulation and release of many inflammatory mediators like cytokines, growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Photodynamic therapy; Extracellular matrix; Adhesion; Chemical mediators; Matrix metalloproteinases.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800002
Imagem não selecionada

Imprime registro no formato completo
Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors BJMBR
Gozzo,A.J.; Nunes,V.A.; Nader,H.B.; Dietrich,C.P.; Carmona,A.K.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S..
Human plasma kallikrein, a serine proteinase, plays a key role in intrinsic blood clotting, in the kallikrein-kinin system, and in fibrinolysis. The proteolytic enzymes involved in these processes are usually controlled by specific inhibitors and may be influenced by several factors including glycosaminoglycans, as recently demonstrated by our group. The aim of the present study was to investigate the effect of glycosaminoglycans (30 to 250 µg/ml) on kallikrein activity on plasminogen and factor XII and on the inhibition of kallikrein by the plasma proteins C1-inhibitor and antithrombin. Almost all available glycosaminoglycans (heparin, heparan sulfate, bovine and tuna dermatan sulfate, chondroitin 4- and 6-sulfates) reduced (1.2 to 3.0 times) the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycosaminoglycans; Human plasma kallikrein; Plasminogen; Factor XII; C1-inhibitor; Antithrombin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800011
Imagem não selecionada

Imprime registro no formato completo
Heparan sulfate and cell division BJMBR
Porcionatto,M.A.; Nader,H.B.; Dietrich,C.P..
Heparan sulfate is a component of vertebrate and invertebrate tissues which appears during the cytodifferentiation stage of embryonic development. Its structure varies according to the tissue and species of origin and is modified during neoplastic transformation. Several lines of experimental evidence suggest that heparan sulfate plays a role in cellular recognition, cellular adhesion and growth control. Heparan sulfate can participate in the process of cell division in two distinct ways, either as a positive or negative modulator of cellular proliferation, or as a response to a mitogenic stimulus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heparan sulfate; Glycosaminoglycan; Proteoglycan; Cell division; Cell cycle; Growth factors.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500006
Imagem não selecionada

Imprime registro no formato completo
Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase BJMBR
Franco,C.R.C.; Rocha,H.A.O.; Trindade,E.S.; Santos,I.A.N.; Leite,E.L.; Veiga,S.S.; Nader,H.B.; Dietrich,C.P..
We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CHO cells; Extracellular matrix proteins; Proteoglycans; Glycosaminoglycans; Cell adhesion; Cell proliferation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800001
Imagem não selecionada

Imprime registro no formato completo
Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates? BJMBR
Nader,H.B.; Chavante,S.F.; dos-Santos,E.A.; Oliveira,F.W.; de-Paiva,J.F.; Jerônimo,S.M.B.; Medeiros,G.F.; de-Abreu,L.R.D.; Leite,E.L.; de-Sousa-Filho,J.F.; Castro,R.A.B.; Toma,L.; Tersariol,I.L.S.; Porcionatto,M.A.; Dietrich,C.P..
The distribution and structure of heparan sulfate and heparin are briefly reviewed. Heparan sulfate is a ubiquitous compound of animal cells whose structure has been maintained throughout evolution, showing an enormous variability regarding the relative amounts of its disaccharide units. Heparin, on the other hand, is present only in a few tissues and species of the animal kingdom and in the form of granules inside organelles in the cytoplasm of special cells. Thus, the distribution as well as the main structural features of the molecule, including its main disaccharide unit, have been maintained through evolution. These and other studies led to the proposal that heparan sulfate may be involved in the cell-cell recognition phenomena and control of cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heparin - occurrence and function; Heparan sulfate - occurrence and function; Heparin - invertebrates; Heparan sulfate - invertebrates; Heparin and heparan sulfate - structure.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500005
Imagem não selecionada

Imprime registro no formato completo
Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression BJMBR
Regatieri,C.V.; Dreyfuss,J.L.; Melo,G.B.; Lavinsky,D.; Hossaka,S.K.; Rodrigues,E.B.; Farah,M.E.; Maia,M.; Nader,H.B..
The objective of the present study was to develop a quantitative method to evaluate laser-induced choroidal neovascularization (CNV) in a rat model using Heidelberg Retina Angiograph 2 (HRA2) imaging. The expression of two heparan sulfate proteoglycans (HSPG) related to inflammation and angiogenesis was also investigated. CNV lesions were induced with argon laser in 21 heterozygous Zucker rats and after three weeks a fluorescein angiogram and autofluorescence exams were performed using HRA2. The area and greatest linear dimension were measured by two observers not aware of the protocol. Bland-Altman plots showed agreement between the observers, suggesting that the technique was reproducible. After fluorescein angiogram, HSPG (perlecan and syndecan-4) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Choroidal neovascularization; Fluorescein angiogram; Proteoglycans; Angiogenesis; Confocal scanning laser ophthalmoscope.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700004
Imagem não selecionada

Imprime registro no formato completo
Specific structural features of syndecans and heparan sulfate chains are needed for cell signaling BJMBR
Lopes,C.C.; Dietrich,C.P.; Nader,H.B..
The syndecans, heparan sulfate proteoglycans, are abundant molecules associated with the cell surface and extracellular matrix and consist of a protein core to which heparan sulfate chains are covalently attached. Each of the syndecan core proteins has a short cytoplasmic domain that binds cytosolic regulatory factors. The syndecans also contain highly conserved transmembrane domains and extracellular domains for which important activities are becoming known. These protein domains locate the syndecan on cell surface sites during development and tumor formation where they interact with other receptors to regulate signaling and cytoskeletal organization. The functions of cell surface heparan sulfate proteoglycan have been centered on the role of heparan...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Syndecans; Heparan sulfate proteoglycans; Protein kinase C; Growth factors; Cell signaling.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200001
Imagem não selecionada

Imprime registro no formato completo
Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy BJMBR
Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L..
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557
Registros recuperados: 11
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional