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Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes Genet. Mol. Biol.
Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva.
Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018
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