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| Kincaid-smith, Julien; Boissier, Jerome; Allienne, Jean-francois; Oleaga, Ana; Djuikwo-teukeng, Felicite; Toulza, Eve. |
| For scientists working on gonochoric organisms, determining sex can be crucial for many biological questions and experimental studies, such as crossbreeding, but it can also be a challenging task, particularly when no sexual dimorphism is visible or cannot be directly observed. In metazoan parasites of the genus Schistosoma responsible for schistosomiasis, sex is genetically determined in the zygote with a female heterogametic ZW/ZZ system. Adult flukes have a pronounced sexual dimorphism, whereas the sexes of the larval stages are morphologically indistinguishable but can be distinguished uniquely by using molecular methods. Therefore, reliable methods are needed to identify the sex of larvae individuals. Here, we present an endpoint PCR-based assay using... |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00372/48333/71858.pdf |
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| Oleaga, Ana; Rey, Olivier; Polack, Bruno; Grech-angelini, Sébastien; Quilichini, Yann; Pérez-sánchez, Ricardo; Boireau, Pascal; Mulero, Stephen; Brunet, Aimé; Rogon, Anne; Vallée, Isabelle; Kincaid-smith, Julien; Allienne, Jean-françois; Boissier, Jérôme. |
| Environmental and anthropogenic changes are expected to promote emergence and spread of pathogens worldwide. Since 2013, human urogenital schistosomiasis is established in Corsica island (France). Schistosomiasis is a parasitic disease affecting both humans and animals. The parasite involved in the Corsican outbreak is a hybrid form between Schistosoma haematobium, a human parasite, and Schistosoma bovis, a livestock parasite. S. bovis has been detected in Corsican livestock few decades ago raising the questions whether hybridization occurred in Corsica and if animals could behave as a reservoir for the recently established parasite lineage. The latter hypothesis has huge epidemiological outcomes since the emergence of a zoonotic lineage of schistosomes... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00504/61566/65491.pdf |
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