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Anti-oxidative and proteolytic activities and protein profile of laticifer cells of Cryptostegia grandiflora, Plumeria rubra and Euphorbia tirucalli Braz. J. Plant Physiol.
Freitas,Cleverson D. T. de; Souza,Diego P. de; Araújo,Eliane S.; Cavalheiro,Mariana G.; Oliveira,Luciana S.; Ramos,Márcio V..
In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifers of P. rubra. This profile was not found in laticifers of C. grandiflora and E. tirucalli. The later was poor in respect of proteins. Strong anti-oxidative activity of superoxide dismutase (E.C. 1.15.1.1) was detected in P. rubra and C. grandiflora latices, and to a lesser extent ascorbate peroxidase (E.C. 1.11.1.1) and isoforms of peroxidase were seen. Catalase (E.C. 1.11.1.6) was detected only in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Chitinase; Cysteine proteinase; Latex; Peroxidase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100002
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The influence of processing and long-term storage on the antioxidant metabolism of acerola (Malpighia emarginata) purée Braz. J. Plant Physiol.
Oliveira,Luciana S.; Rufino,Maria S. M.; Moura,Carlos F. H.; Cavalcanti,Fabio R.; Alves,Ricardo E.; Miranda,Maria R. A..
This study evaluated the effect of the processing and long-term storage on the antioxidant potential and activity of antioxidant enzymes of frozen purées from six acerola clones. Ripe acerolas from clones BRS 235, BRS 236, BRS 237, BRS 238, II47/1 and BRS 152 were harvested; the pulp was processed, packed in sealed polyethylene plastic bags and stored in a domestic freezer at -18°C for 11 months. Samples of each clone were analyzed on harvest/processing day and every 30 days after for bioactive antioxidant compounds, antioxidant enzyme activity and total antioxidant activity. Acerola purées presented a decrease of non-enzyme antioxidants and an increase of antioxidant enzymes activities, indicatives of a compensatory mechanism between enzymatic and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pulp; Enzymes; Vitamin C; Postharvest; Frozen.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200007
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