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| NOGUEIRA, L. M.; HOFFMANN-CAMPO, C. B.; PASSIANOTTO, A. L. de L.; GRAÇA, J. P. da; ABRÃO, M. Z.; PEREIRA, S. dos S.; SILVA, D. C. G. da; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; YAMANAKA, N.. |
| 2006 |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Ferrugem asiática; Soja. |
| Ano: 2006 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/469870 |
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| STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.; PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.. |
| The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Estabilidade de expressão; Expressão gênica; RT-qPCR; Soja; Biotecnologia; Deficiência hídrica; Soybeans; Gene expression; Plant-water relations; Agricultural biotechnology. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/882641 |
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| BRITO, L. G.; NERY, L. de O.; BARBIERI, F. da S.; HUACCA, M. E. F.; PEREIRA, S. dos S.; SILVA, R. R. da; FERNANDES, C. F. C.; OLIVEIRA, M. C. de S.. |
| The use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Cattle tick; Organophosphate resistance; Esterases. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074923 |
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