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In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). Repositório Alice
CALEGARIO, R. F.; LABATE, M.T.V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W..
Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus; Citrus leprosis virus C; Brevipalpus phoenicis; Cilevirus; Citrus sinensis; Polyclonal antibody; Serology.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937928
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