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Draft genome sequence of Streptomyces sp. strain F1, a potential source for glycoside hydrolases isolated from Brazilian soil BJM
Melo,Ricardo Rodrigues de; Persinoti,Gabriela Felix; Paixão,Douglas Antonio Alvaredo; Squina,Fábio Márcio; Ruller,Roberto; Sato,Helia Harumi.
ABSTRACT Here, we show the draft genome sequence of Streptomyces sp. F1, a strain isolated from soil with great potential for secretion of hydrolytic enzymes used to deconstruct cellulosic biomass. The draft genome assembly of Streptomyces sp. strain F1 has 69 contigs with a total genome size of 8,142,296 bp and G + C 72.65%. Preliminary genome analysis identified 175 proteins as Carbohydrate-Active Enzymes, being 85 glycoside hydrolases organized in 33 distinct families. This draft genome information provides new insights on the key genes encoding hydrolytic enzymes involved in biomass deconstruction employed by soil bacteria.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinobacteria; Glycoside hydrolases; Streptomyces; Draft genome sequence; Soil bacteria.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400612
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Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation Rev. Bras. Ciênc. Solo
Paixão,Douglas Antonio Alvaredo; Dimitrov,Mauricio Rocha; Pereira,Rodrigo Matheus; Accorsini,Fábio Raphael; Vidotti,Maria Benincasa; Lemos,Eliana Gertrudes de Macedo.
Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Cloning; 16S rRNA; Metagenomics; Soil; Biosurfactants.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300019
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