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Influence of exogenous L-proline on embryogenic cultures of larch (Larix leptoeuropaea Dengler), sitka spruce (Picea sitchensis (Bong.) Carr.) and oak (Quercus robur L.) subjected to cold and salt stress Inra
Gleeson, D.; Lelu-Walter, M.A.; Parkinson, M..
Des cultures embryogènes de mélèze hybride, d'épicéa de sitka et de chêne ont été soumises à différentes conditions de culture et leur croissance étudiée en fonction de l'ajout de L-proline au milieu de culture (1 mM, 10 mM, et 100 mM). Si des températures basses (4 °C) inhibent totalement la croissance des cultures, celle-ci redevient partiellement normale en présence de proline. Nos résultats montrent que les cultures non seulement survivent à de basses températures mais sont aussi capables de croître activement au cours de la durée d'application du froid. De même, la croissance est inversement corrélée à la concentration en sel avec sa complète inhibition en présence de 200 mM de NaCl. L'addition de L-proline au milieu de culture (quelles que soient les...
Tipo: Journal Article Palavras-chave: MELEZE HYBRIDE; PROLINE; TOLERANCE AU STRESS; FROID; SEL; MILIEU DE CULTURE LARIX LEPTOEUROPAEA; QUERCUS ROBUR.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200795cc7593&uri=/notices/prodinra1/2009/11/
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Overproduction of proline in transgenic hybrid larch (Larix x leptoeuropaea (Dengler) cultures renders them tolerant to cold, salt and frost Inra
Gleeson, D.; Lelu-Walter, M.A.; Parkinson, M..
Cold, salt and frost are important environmental stresses in forest trees and may significantly reduce productivity. Elevated levels of proline are associated with these stresses and may help alleviate their effects. Transgenic hybrid larch Larix leptoeuropaea has been produced expressing a Vigna aconitifolia gene for pyrroline 5-carboxylate synthase, the rate-limiting step in proline synthesis. Embryogenic masses of hybrid larch were co-cultivated with Agrobacterium tumefaciens harbouring a binary vector expressing the gene. The integration of the gene into the plant genome was confirmed by Southern blot and by proline content analysis. There was an approximately 30-fold increase in proline level in transgenic tissue compared to non-transformed controls....
Tipo: Journal Article Palavras-chave: MELEZE HYBRIDE; TRANSGENIQUE; EXPRESSION DES GENES; PROLINE; TOLERANCE AU STRESS; FROID; SEL; GEL LARIX LEPTOEUROPAE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073c8098df&uri=/notices/prodinra1/2007/04/
Registros recuperados: 2
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