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Lactate dehydrogenase as a marker of Plasmodium infection in malaria vector Anopheles IRD
Riandey, Marie-France; Sannier, Christiane; Peltre, G.; Monteny, Nicole; Cavaleyra, Mireille.
Lactate dehydrogenase (Ldh) electrophoresis showed the presence of #Plasmodium yoelii yoelii$ in #Anopheles stephensi$ and #An. gambiae$. The Ldh appeared as an additional band (pLdh) whose activity was more intense with 3-acetyl pyridine adenine dinucleotide as coenzyme than with beta nicotinamide adenine dinucleotide. Several allelic forms occurred both in the vector and the host. The isoelectric point of Ldh, similar in the vector and host, differed from those of Ldh from mosquito and mouse. The presence of pLdh was detected from the 2nd to the 28th day of infection. The pLdh appeared to be proportional to the number of sporozoites present in infected salivary glands. However, pLdh was not found in salivary glands or midguts, but it was detected in the...
Tipo: Text Palavras-chave: ENTOMOLOGIE MEDICALE; PALUDISME; EPIDEMIOLOGIE; INFECTION; ENZYME; DOSAGE; LACTATE DESHYDROGENASE; ELECTROPHORESE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010006783
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