|
|
|
| Registros recuperados: 51 | |
|
|
| Goudenege, David; Travers, Marie-agnes; Lemire, Astrid; Petton, Bruno; Haffner, Philippe; Labreuche, Yannick; Tourbiez, Delphine; Mangenot, Sophie; Calteau, Alexandra; Mazel, Didier; Nicolas, Jean-louis; Jacq, Annick; Le Roux, Frederique. |
| Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that... |
| Tipo: Text |
|
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00226/33685/32530.pdf |
| |
|
|
| Lafont, Maxime; Vergnes, Agnes; Vidal-dupiol, Jeremie; De Lorgeril, Julien; Gueguen, Yannick; Haffner, Philippe; Petton, Bruno; Chaparro, Cristian; Barrachina, Celia; Destoumieux-garzon, Delphine; Mitta, Guillaume; Gourbal, Benjamin; Montagnani, Caroline. |
| Over the last decade, innate immune priming has been evidenced in many invertebrate phyla. If mechanistic models have been proposed, molecular studies aiming to substantiate these models have remained scarce. We reveal here the transcriptional signature associated with immune priming in the oyster Crassostrea gigas. Oysters were fully protected against Ostreid herpesvirus 1 (OsHV-1), a major oyster pathogen, after priming with poly(I·C), which mimics viral double-stranded RNA. Global analysis through RNA sequencing of oyster and viral genes after immune priming and viral infection revealed that poly(I·C) induces a strong antiviral response that impairs OsHV-1 replication. Protection is based on a sustained upregulation of immune genes, notably genes... |
| Tipo: Text |
Palavras-chave: Innate immunity; Priming; OsHV-1; Antiviral response; Immune memory; Oyster; POMS; Poly(I·C); Interferon; Transcriptomic. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00614/72580/71541.pdf |
| |
|
|
| Bruto, Maxime; Labreuche, Yannick; James, Adele; Piel, Damien; Chenivesse, Sabine; Petton, Bruno; Polz, Martin F.; Le Roux, Frederique. |
| Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00452/56370/57967.pdf |
| |
|
|
| Cochennec-laureau, Nathalie; Baud, Jean-pierre; Bedier, Edouard; Boudry, Pierre; Huvet, Arnaud; Nicolas, Jean-louis; Pepin, Jean-francois; Petton, Bruno. |
| Ce bilan comprend deux parties : la première est dédiée à une synthèse des travaux présentés lors des journées « Surmortalités des huîtres creuses, Crassostrea gigas » les 8 et 9 décembre 2009 et, la seconde partie, à une proposition d'un projet de recherche spécifique 2010-2012 en complément des propositions de « sortie de crise ». Contexte Les mortalités estivales de l'huître creuse, Crassostrea gigas, sont décrites depuis une vingtaine d'années en France mais également au Japon, en Corée, aux USA et en Australie. Ces phénomènes ou « syndrome des mortalités estivales » ont fait l'objet d'une étude pluridisciplinaire en France entre 2001 et 2006 (Défi MOREST). Toutefois, en 2008 et 2009, l'ostréiculture française a du faire face à deux épisodes de... |
| Tipo: Text |
|
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2010/acte-7393.pdf |
| |
|
|
| Suquet, Marc; Omnes, Marie-helene; Normant, Yvon; Petton, Bruno; Severe, Armelle; Fauvel, Christian; Barone, Herve; Quemener, Loic; Buchet, Vincent; Pasco, Laurent; Menard, E; Gaignon, Jean-louis. |
| Because of its rapid growth, its good reputation and the promising aquaculture experiences conducted in Norway and Scotland, cod is considered as a good candidate for cold water aquaculture. The aim of this paper is to present the work carried out in France since 1999: the selection of cod as a promising candidate for aquaculture and the assessment of its rearing performances in this environment. |
| Tipo: Text |
|
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00170/28142/26362.pdf |
| |
|
|
| Gangnery, Aline; Normand, Julien; Duval, Cyrielle; Cugier, Philippe; Grangeré, Karine; Petton, Bruno; Petton, Sebastien; Orvain, Francis; Pernet, Fabrice. |
| Oyster diseases have major consequences on fisheries and aquaculture. In France, young Pacific oysters Crassostrea gigas are severely hit by the ostreid herpesvirus, whereas adults suffer mortalities presumably caused by pathogenic bacteria. Here we investigated the origin and spread of mortalities that affect both young and adult oysters, and we identified and compared their risk factors. Mortality was monitored in 2 age classes of oysters deployed in early spring at 39 sites spread over a 37 km2 surface area inside and outside of shellfish farms. Environmental data obtained from numerical modelling were used to investigate risk factors. Mortality of young oysters associated with ostreid herpesvirus occurred in the oyster farming area. Hydrodynamic... |
| Tipo: Text |
Palavras-chave: Aquaculture; Bivalve; Disease; Epidemiology and health; Ecological modelling; Hydrodynamic connectivity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/69983/67896.pdf |
| |
|
|
| Petton, Bruno; Bruto, Maxime; James, Adele; Labreuche, Yannick; Alunno-bruscia, Marianne; Le Roux, Frederique. |
| Successive disease outbreaks in oyster (Crassostrea gigas) beds in France have resulted in dramatic losses in production, and subsequent decline in the oyster-farming industry. Deaths of juvenile oysters have been associated with the presence of a herpes virus (OsHV-1 µvar) and bacterial populations of the genus Vibrio. Although the pathogenicity of OsHV-1 µvar, as well as several strains of Vibrio has been demonstrated by experimental infections, our understanding of the complexity of infections occurring in the natural environment remains limited. In the present study, we use specific-pathogen-free (SPF) oysters infected in an estuarine environment to study the diversity and dynamics of cultured microbial populations during disease expression. We observe... |
| Tipo: Text |
Palavras-chave: Pacific oysters; Summer mortality; Herpes virus; Vibrio pathogenicity; Experimental infection. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38220/36377.pdf |
| |
|
|
| Pernet, Fabrice; Fuhrmann, Marine; Petton, Bruno; Mazurie, Joseph; Bouget, Jean-francois; Fleury, Elodie; Daigle, Gaetan; Gernez, Pierre. |
| Marine diseases have major impacts on ecosystems and economic consequences for aquaculture and fisheries. Understanding origin, spread and risk factors of disease is crucial for management, but data in the ocean are limited compared to the terrestrial environment. Here we investigated how the marine environment drives the spread of viral disease outbreak affecting The Pacific oyster worldwide by using a spatial epidemiology framework. We collected environmental and oyster health data at 46 sites spread over an area of 300 km2 along an inshore-offshore gradient during an epizootic event and conducted risk analysis. We found that disease broke out in the intertidal farming area and spread seaward. Mortalities and virus detection were observed in oysters... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00450/56130/57670.pdf |
| |
|
|
| Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis. |
| The first aim of this study was to determine the stocking density limits for Pacific oyster Crassostrea gigas larvae reared in flow-through system (FTS) and recirculating aquaculture systems (RAS). The second aim was to examine biofilm formation on the larval tank wall and its interaction with larvae growth. Three larvae concentrations were tested: 50, 150, and 300 mL−1. Chemical parameters and larvae performance were measured. The biofilm was observed by scanning electron microscopy, and its bacterial composition was investigated by pyrosequencing analysis of part of the 16S rRNA gene. The highest growth (13 µm day–1), survival (87%) and metamorphosis (50%) rates were observed in FTS at 50 larvae mL–1, while lower and similar performances occurred at... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Larval culture; High stocking density; Biofilm; Filamentous bacteria. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50611/51324.pdf |
| |
|
|
| De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Perez, Valérie; Stenger, Pierre-louis; Degremont, Lionel; Montagnani, Caroline; Escoubas, Jean Michel; Haffner, Philippe; Allienne, Jean-françois; Leroy, Marc; Lagarde, Franck; Vidal Dupiol, Jeremie; Gueguen, Yannick; Mitta, Guillaume. |
| Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Oyster disease; Resistance; OsHV-1; Antiviral molecular pathways; Invertebrate immunity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00602/71369/69811.pdf |
| |
|
| |
|
| |
|
|
| Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis. |
| This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial... |
| Tipo: Text |
Palavras-chave: RAS; Bivalve larvae; Microbiota; Pyrosequencing; Hatchery. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00346/45691/45311.pdf |
| |
|
|
| Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice. |
| Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented... |
| Tipo: Text |
Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf |
| |
|
|
| Pernet, Fabrice; Gachelin, Sonia; Stanisière, Jean-yves; Petton, Bruno; Fleury, Elodie; Mazurié, Joseph. |
| The intertidal zone is characterized by a sharp vertical gradient of environmental stress, which structures species distribution and their interactions. Few studies, however, have examined the influence of tidal height on host–pathogen interactions. Here, we investigated how the tidal height influence outbreak of the Ostreid herpesvirus type 1 (OsHV-1) affecting the Pacific oyster. A volunteer network composed of 20 oyster growers monitored the survival of 28 batches of oysters during an epizootic event in Southern Brittany, France. Oysters were spat from wild collection or hatchery production. The sampling sites were spread over a 150-km2 area with a tidal height ranging from 0.98 to 2.90 m. Concomitantly, we followed survival of oyster spats in relation... |
| Tipo: Text |
Palavras-chave: Aquaculture; Citizen science; Marine epidemiology and health; OsHV-1; Risk analysis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60271/63981.pdf |
| |
|
| |
|
|
| De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume. |
| Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf |
| |
|
|
| Gaignon, Jean-louis; Petton, Bruno; Quemener, Loic. |
| During larval rearing phase of turbot, a substantial variability in survival and growth is frequently observed. In this paper, we studied the effects of hydrodynamics in larvae rearing tanks on the survival and the growth of fed and starved larvae, using the following conditions: water flows of 600 and 1200 ml.mn(-1), constant and progressive ones, air flows of 10 and 30 ml.mn(-1), downward and upward water circulation, with direction change during the rearing phase. We also studied the interactions between water circulation direction and feeding conditions by (1) shifting the first feeding day (4 occasions, at 8 hours intervals, from 52 to 76 hours after 95% of hatching) and (2) using 2 amounts of preys (which differ by a factor 3). The results show that... |
| Tipo: Text |
Palavras-chave: Turbot; Techniques d'élevage; Alimentation; Turbot; Rearing methods; Feeding. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00447/55837/62120.pdf |
| |
|
|
| Gonzalez, Ricardo; Mingant, Christian; Petton, Bruno; Robert, Rene. |
| In contrast with the Japanese oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum, Ostrea edulis seed production in the hatchery has been reported to be erratic, with sudden and unexplained larval and post-metamorphosis mortalities. Fecundity and initial larval quality have been related to broodstock conditioning, but effects on larval development and metamorphosis remain poorly understood. In addition, molluscan larval mortalities have been often associated with bacterial contamination and flow-through techniques may help to overcome this problem. Both aspects have been considered in the present work. O. edulis broodstock were conditioned at 19 °C and fed three different microalgal diets. Two were single-species diets: Rhodomonas salina... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Feeding; Broodstock conditioning; Larval development; Flow-through. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00090/20093/17732.pdf |
| |
|
|
| Robert, Rene; Vignier, Julien; Petton, Bruno. |
| Under controlled conditions of food density and temperature, larval performances (ingestion, growth, survival and settlement success) of the flat oyster, Ostrea edulis, were investigated using a flow-through rearing system. In the first experiment, oyster larvae were reared at five different phytoplankton densities (70, 500, 1500, 2500 and 3500 μm3 μL−1: ≈1, 8, 25, 42 and 58 cells μL−1 equivalent TCg), and in the second, larvae were grown at four different temperatures (15, 20, 25 and 30°C). Overall, larvae survived a wide range of food density and temperature, with high survival recorded at the end of the experiments. Microalgae concentration and temperature both impacted significantly larval development and settlement success. A mixed diet of Chaetoceros... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Larvae; Temperature; Feeding regime; Flow-through; DEB parameters. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00377/48817/49295.pdf |
| |
| Registros recuperados: 51 | |
|
|
|
