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| Lopez, C.; Jorge, V.; Piégu, B.; Mba, C.; Cortes, D.; Restrepo, S.; Soto, M.; Laudié, M.; Berger, C.; Cooke, R.; Delseny, M.; Tohme, J.; Verdier, V.. |
| Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv. manihotis. We report here the single pass sequencing of 11 954 cDNA clones from the 5' ends, including 111 from the 3' ends. Cluster analysis permitted the identification of a unigene set of 5700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas ~16% sequences did not show any significant similarity with other proteins present in... |
| Tipo: Journal Article |
Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; EXPRESSION DES GENES; RESSOURCE GENOMIQUE; BACTERIOSE. |
| Ano: 2004 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200758ab2534&uri=/notices/prodinra1/2007/04/ |
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| Anderson, J.V.; Delseny, M.; Fregene, M.A.; Jorge, V.; Mba, C.; Lopez, C.; Restrepo, S.; Soto, M.; Piégu, B.; Verdier, V.; Cooke, R.; Tohme, J.. |
| Cassava (Manihot esculenta) is a major food staple for nearly 600 million people in Africa, Asia, and Latin America. Major losses in yield result from biotic and abiotic stresses that include diseases such as Cassava Mosaic Disease (CMD) and Cassava Bacterial Blight (CBB), drought, and acid soils. Additional losses also occur from deterioration during the post-harvest storage of roots. To help cassava breeders overcome these obstacles, the scientific community has turned to modern genomics approaches to identify key genetic characteristics associated with resistance to these yield-limiting factors. One approach for developing a genomics program requires the development of ESTs (expressed sequence tags). To date, nearly 23000 ESTs have been developed from... |
| Tipo: Journal Article |
Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; SEQUENCE DE GENES; RESSOURCE GENOMIQUE. |
| Ano: 2004 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e657bf31&uri=/notices/prodinra1/2007/04/ |
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