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Registros recuperados: 10
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Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the ProCount™-BD and ISHAGE protocols BJMBR
Pranke,P.; Hendrikx,J.; Alespeiti,G.; Nardi,N.; Rubinstein,P.; Visser,J..
The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/µL and with the ProCount™ method...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoietic stem cell; Human umbilical cord blood; CD34+; ISHAGE; ProCount.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700008
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Development of VEGF-loaded PLGA matrices in association with mesenchymal stem cells for tissue engineering BJMBR
Rosa,A.R.; Steffens,D.; Santi,B.; Quintiliano,K.; Steffen,N.; Pilger,D.A.; Pranke,P..
The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured. The nanofibers showed smooth surfaces without beads and with interconnected pores. PLGA/BSA/VEGF showed the smallest water contact...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Emulsion electrospinning; VEGF; Stem cells; PLGA; Biomaterials.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900601
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Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion BJMBR
Paim,A.; Braghirolli,D.I.; Cardozo,N.S.M.; Pranke,P.; Tessaro,I.C..
Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on human dental pulp stem cell detachment. Polycaprolactone scaffolds were electrospun using a solvent mixture of chloroform and methanol. The viable cell number was determined at each tested condition. Cell morphology was analyzed by confocal microscopy after various incubation times for static cell adhesion with a high seeding density. Scanning electron microscopy images were obtained before and after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell adhesion; Perfusion; Shear stress; Stem cell; Electrospun scaffolds.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500615
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Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury BJMBR
Nicola,F.C.; Rodrigues,L.P.; Crestani,T.; Quintiliano,K.; Sanches,E.F.; Willborn,S.; Aristimunha,D.; Boisserand,L.; Pranke,P.; Netto,C.A..
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spinal cord injury; Stem cells; Treadmill training; Functional recovery; MASCIS impactor.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900604
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Immunophenotype of hematopoietic stem cells from placental/umbilical cord blood after culture BJMBR
Pranke,P.; Hendrikx,J.; Debnath,G.; Alespeiti,G.; Rubinstein,P.; Nardi,N.; Visser,J..
Identification and enumeration of human hematopoietic stem cells remain problematic, since in vitro and in vivo stem cell assays have different outcomes. We determined if the altered expression of adhesion molecules during stem cell expansion could be a reason for the discrepancy. CD34+CD38- and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin (TPO), FLT-3 ligand (FL) and kit ligand (KL; or stem cell factor) in different combinations: TPO + FL + KL, TPO + FL and TPO, at concentrations of 50 ng/mL each. Cells were immunophenotyped by four-color fluorescence using antibodies against CD11c, CD31, CD49e, CD61, CD62L, CD117, and HLA-DR. Low-density cord blood contained 1.4 ± 0.9% CD34+ cells, 2.6 ± 2.1% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematopoietic stem cells; CD34+CD38- cells; Human umbilical cord blood; Ex vivo expansion; Adhesion molecules.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200006
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Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts BJMBR
Braghirolli,D.I.; Caberlon,B.; Gamba,D.; Petry,JFTC.; Dias,M.L.; Pranke,P..
Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poly(trimethylene carbonate-co-L-lactide); Vascular scaffolds; Mesenchymal stem cells; Endothelial progenitor cells; Smooth muscle cells.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800611
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The use of stem cells for the treatment of autoimmune diseases BJMBR
Rosa,S.B.; Voltarelli,J.C.; Chies,J.A.B.; Pranke,P..
Autoimmune diseases constitute a heterogeneous group of conditions commonly treated with anti-inflammatory, immunosuppressant and immunomodulating drugs, with satisfactory results in most cases. Nevertheless, some patients become resistant to conventional therapy. The use of high doses of drugs in such cases results in the need for bone marrow reconstitution, a situation which has stimulated research into the use of hematopoietic stem cells in autoimmune disease therapy. Stem cell transplantation in such diseases aims to destroy the self-reacting immune cells and produce a new functional immune system, as well as substitute cells for tissue damaged in the course of the disease. Significant results, such as the reestablishment of tolerance and a decrease in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autoimmune disease; Stem cells; Transplantation; Lupus erythematosus; Arthritis; Type I diabetes mellitus.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200001
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Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats BJMBR
Rodrigues,L.P.; Iglesias,D.; Nicola,F.C.; Steffens,D.; Valentim,L.; Witczak,A.; Zanatta,G.; Achaval,M.; Pranke,P.; Netto,C.A..
Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 10(6) cells diluted in 10 µL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spinal cord injury; Cell therapy; Functional recovery; Mononuclear cells; Human umbilical cord blood; MASCIS impactor.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100009
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Viability of mesenchymal stem cells during electrospinning BJMBR
Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P..
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006
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VPA/PLGA microfibers produced by coaxial electrospinning for the treatment of central nervous system injury BJMBR
Reis,K.P.; Sperling,L.E.; Teixeira,C.; Sommer,L.; Colombo,M.; Koester,L.S.; Pranke,P..
The central nervous system shows limited regenerative capacity after injury. Spinal cord injury (SCI) is a devastating traumatic injury resulting in loss of sensory, motor, and autonomic function distal from the level of injury. An appropriate combination of biomaterials and bioactive substances is currently thought to be a promising approach to treat this condition. Systemic administration of valproic acid (VPA) has been previously shown to promote functional recovery in animal models of SCI. In this study, VPA was encapsulated in poly(lactic-co-glycolic acid) (PLGA) microfibers by the coaxial electrospinning technique. Fibers showed continuous and cylindrical morphology, randomly oriented fibers, and compatible morphological and mechanical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coaxial electrospinning; VPA; Spinal cord injury.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400606
Registros recuperados: 10
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