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Evaluation of the Resistance of Transgenic C5 Plum (Prunus domestica L.) against Four Chilean Plum Pox Virus Isolates through Micro-Grafting Chilean J. Agric. Res.
Wong,Wendy; Barba,Paola; Álvarez,Catalina; Castro,Álvaro; Acuña,Manuel; Zamora,Pablo; Rosales,Marlene; Dell´Orto,Paola; Moynihan,Michael R; Scorza,Ralph; Prieto,Humberto.
The transgenic plum (Prunus domestica L.) C5, in which the coat protein (CP) gene of the Plum pox virus (PPV) is inserted, represents a unique example of the use of genetic engineering for fruit crop improvement in Prunus spp. Field trials in Poland, Romania, and Spain have demonstrated resistance of C5 to several D and M strain PPV isolates. In Chile, the quarantine regulations for PPV and for genetically modified (GM) plants require that the testing of C5 for resistance to Chilean PPV isolates be done under controlled isolated conditions. To carry out these tests C5 shoots were multiplied in vitro and micro-grafted onto four Adesoto101 (Prunus insititia L.) rootstock populations that had been previously infected each with one of four Chilean PPV-Ds....
Tipo: Journal article Palavras-chave: C5; Chilean isolates; Micro-grafting.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000300004
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Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor Electron. J. Biotechnol.
Sequeida,Álvaro; Tapia,Eduardo; Ortega,Marcelo; Zamora,Pablo; Castro,Álvaro; Montes,Christian; Zúñiga,Gustavo E; Prieto,Humberto.
Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity...
Tipo: Journal article Palavras-chave: Deschampsia antarctica; Phenolic compounds; Temporary immersion bioreactor; UV-B elicitation.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400008
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