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Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification Biological Sciences
Prudente, Débora de Oliveira; Paiva, Renato; Nery, Fernanda Carlota; Máximo, Wesley Pires Flausino; Silva, Luciano Coutinho.
 Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Botânica criopreservação de recursos genéticos vegetais Mangabeira; Recalcitrant species; Long-term conservation; Dehydration. Botânica.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620
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In vitro germination and seed cryopreservation of paineira-rosa Sci. Agrar.Paran. / SAP
Prudente, Débora de Oliveira; Nery, Fernanda Carlota; Silva, Laís Silveira da; Paiva, Renato; Reis, Michele Valquíria dos; Nery, Marcela Carlota.
The paineira-rosa (Chorisia speciosa St. Hil) is an arboreal species of great importance in restoring degraded ecosystems and riparian forests, however, the availability of seeds of this species is low. The objective was to establish in vitro germination protocols and cryopreservation of paineira-rosa seeds. For the in vitro germination, MS culture medium and WPM was tested three concentrations of sucrose (0, 15, 30 g L-1) and three pH levels (4.8, 5.8 and 6.8). For cryopreservation, the seeds were dried on silica gel or laminar flow for different times (0, 1, 2, 3 and 4 h) and then were stored in liquid nitrogen (-196 °C) for 24 h, defrosted in a water bath (38 °C) for four minutes and they were inserted into test tubes containing WPM medium cultivation....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Armazenamento em longo prazo; Chorisia speciosa; Dessecação; Espécie florestal..
Ano: 2016 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11775
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Micropropagation of candeia, native species from brazilian cerrado Sci. Agrar.Paran. / SAP
Prudente, Débora de Oliveira; Nery, Fernanda Carlota; Paiva, Renato; Goulart, Vívian Loryane Ávila; Anselmo, Aline Cristini do Nascimento.
Candeia (Eremanthus elytropappus) is a native species from Brazilian Cerrado and presenting economic potential for the timber and pharmaceutical industry. However, the ex vitro germination rate of the seeds is considered low. In this context, the objective was to establish a protocol for in vitro germination and multiplication, aiming at the rapid multiplication of the species. For the in vitro germination medium were tested WPM; ½ WPM; ¼ WPM; MS; MS ½ and ¼ MS. In addition, Were Also tested GA3 (0.0; 0.2; 0.4; 0.6; 0.8 mg L-1) and pH levels (4.8, 5.8 and 6.8) in ¼ WPM culture medium. For in vitro multiplication, stem segments were inoculated in ¼ WPM supplemented with BAP (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1). Shoots obtained in vitro were individually...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Cultura de tecidos; Eremanthus erythropappus (DC.) MacLeish; Espécie florestal..
Ano: 2016 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/12893
Registros recuperados: 3
Primeira ... 1 ... Última
 

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