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Molecular biology of baculovirus and its use in biological control in Brazil PAB
Castro,Maria Elita Batista de; Souza,Marlinda Lobo de; Sihler,William; Rodrigues,Júlio Carlyle Macedo; Ribeiro,Bergmann Morais.
Baculoviruses are insect viruses found mainly in Lepidoptera. The family Baculoviridae is taxonomically divided in two genera, Nucleopolyhedrovirus and Granulovirus, which differ by occlusion body morphology. NPVs (Nucleopolyhedroviruses) have polyhedrical inclusion bodies (PIBs) containing multiple viral particles, while GVs (Granuloviruses) appear to be generally single particles occluded in oval shaped occlusion bodies. During the life cycle, two different viral progenies are produced: BV (Budded Virus) and PDV (Polyhedra Derived Virus), which are essential for the infectious process and virus propagation in host cells. Baculoviruses are being used for pest control and they are especially safe due to their specificity and invertebrate-restricted host...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nucleopolyhedrovirus; AgNPV; Anticarsia gemmatalis; Baculovirus replication; Gene expression; Viral insecticide.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001000001
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Production of Brazilian human norovirus VLPs and comparison of purification methods BJM
Lamounier,Thais Alves da Costa; Oliveira,Layssa Miranda de; Camargo,Brenda Rabello de; Rodrigues,Kelly Barreto; Noronha,Eliane Ferreira; Ribeiro,Bergmann Morais; Nagata,Tatsuya.
Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Norwalk virus; VLP; Ion-exchange chromatography.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265
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