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A Dynamic Energy Budget (DEB) growth model for Pacific oyster larvae, Crassostrea gigas ArchiMer
Rico-villa, Benjamin; Bernard, Ismael; Robert, Rene; Pouvreau, Stephane.
Tipo: Text Palavras-chave: Dynamic Energy Budget; Crassostrea gigas; Bivalve larvae; Ecophysiology; Growth.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00009/11996/9040.pdf
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A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae ArchiMer
Rico Villa, Benjamin; Woerther, Patrice; Mingant, Christian; Le Piver, David; Pouvreau, Stephane; Hamon, Michel; Robert, Rene.
While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared...
Tipo: Text Palavras-chave: Temperature; Ecophysiology; Bivalve larvae; Crassostrea gigas; Flow through.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4167.pdf
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A glimpse on the mollusc industry in Europe ArchiMer
Robert, Rene; Sanchez, Jose Luis; Perez-paralle, Luz; Ponis, Emanuele; Kamermans, Pauline; O'Mahoney, Maria.
Tipo: Text
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00126/23737/21744.pdf
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A New Photobioreactor for Continuous Microalgal Production in Hatcheries Based on External-Loop Airlift and Swirling Flow ArchiMer
Loubiere, K; Olivo, Erell; Bougaran, Gael; Pruvost, J; Robert, Rene; Legrand, J.
This study deals with the scale of a new photobioreactor for continuous microalgal production in hatcheries. The combination of the state-of-art with the constraints inherent to hatcheries has turned the design into a closed, artificially illuminated and external-loop airlift configuration based on a succession of elementary modules, each one being composed of two transparent vertical interconnected columns. The liquid circulation is ensured pneumatically (air injections) with respect to a swirling motion (tangential inlets). A single module of the whole photobioreactor was built-up to scale its geometry (diameter and length) and to optimize its design (air sparger, tangential inlets). The volumetric productivities were predicted by modeling radiative...
Tipo: Text Palavras-chave: Isochrysis affinis galbana; Swirling flow; Airlift; Hatchery; Continuous photobioreactor.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-5288.pdf
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A physiological and biochemical approach to selecting the ideal diet for Ostrea edulis (L.) broodstock conditioning (part A) ArchiMer
Gonzalez-araya, Ricardo; Queau, Isabelle; Quere, Claudie; Moal, Jeanne; Robert, Rene.
To select the best microalgae for Ostrea edulis conditioning, four single species diets were fed to batches of broodstock, which were then compared using physiological and biochemical approaches. Ingestion and absorption were first studied, showing that Chaetoceros gracilis and Skeletonema marinoï were efficiently ingested (4.9–5.3 mg g−1 h−1) and absorbed (1.9–2.5 mg g−1 h−1) while Tetraselmis suecica led to the lowest physiological responses (0.36 and 0.12 mg g−1 h−1 respectively). When O. edulis were fed any microalgae except T-ISO with only 79 mg g−1, gonads accumulated carbohydrates from 116 to 141 mg g−1 and the extent of accumulation depended on the species supplied in the order T. suecica<C. gracilis<S. marinoï. When oysters were fed either...
Tipo: Text Palavras-chave: Ostrea edulis; Conditioning; Algal diets; Ingestion; Absorption; Biochemical composition.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00033/14449/11748.pdf
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Action de l'acétate de tributyle-étain sur les oeufs et larve D de deux mollusques d'intérêt commercial : Crassostrea gigas (Thunberg) et Mytilus galloprovincialis (Lmk) ArchiMer
Robert, Rene; His, Edouard.
L'acétate de tributyle-étain, qui entre dans la composition de certaines peintures antisalissures, est fortement toxique pour les oeufs et les larves D de Crassostrea gigas a la teneur de 5 p.p.b. (HIS et. ROBERT, 1980). Des concentrations inférieures, aussi basses que 1 microgramme par litre, ont été testées sur cette même espèce, mais aussi sur la moule, Mytilus galloprovincialis.
Tipo: Text
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00091/20268/17912.pdf
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Action de l'acétate de tributyle-étain sur les oeufs et les larves D de deux mollusques d'intérêt commercial : Crassostrea gigas (Thunberg) et Mytilus galloprovincialis (Lmk) ArchiMer
Robert, Rene; His, Edouard.
The Tributyl tin acetate, a component of certain antifouling paints, has deleterious effects on eggs and D shaped larvae from Crassostrea gigas at 5 p.p.b. (HIS & ROBERT, 1980). Here we have tested concentrations as low as 1 p.p.b. on eggs and on 24 hours old larvae both from Crassostrea gigas and Mytilus galloprovincialis. The same results are got ; this product kills embryos and larvae in a few days ; it prevents growth of 24 hours old larvae exposed to this toxic. Mussels larvae seem to be less sensitive.
Tipo: Text Palavras-chave: Antifouling; Larves; Pollution; Toxicité; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis; Antifouling; Larvae; Pollution; Toxicity; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00037/14862/12185.pdf
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Action de l'érythromycine et de l'acide oxolinique sur le déveloloppement larvaire de la coquille St Jacques Pecten maximus ArchiMer
Robert, Rene; Nicolas, Jean-louis; Conan, Rony.
For lack of antibiotics, King scallop larviculture faces high mortalities. In the experimental mollusc hatchery of Argenton (Brittany, France), the presence of a vibrio, Vibrio pectinicida, associated with the stocks seems to be responsible for this low survival rate. As prophylactic methods are difficult to implement in the current structures (water sterilization, egg decontamination, isolation of the stocks), the addition of chloramphenicol is the only solution to protect the stocks. However, the use of this antibiotic, considered as dangerous for man, has been prohibited in animal production throughout the whole of Europe since 1994. The action of two other antibiotics, the erythromycine and the oxolinic acid, has been searched for in Pecten maximus...
Tipo: Text Palavras-chave: Acide oxolinique; Erythromycine; Antibiotiques; Coquille St Jacques; Pecten maximus L.; Développement larvaire.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-6199.PDF
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Action d'un sel organo-métallique, l'acétate de tributyle-étain sur les oeufs et les larves D de Crassostrea gigas (Thunberg) ArchiMer
His, Edouard; Robert, Rene.
L'action de l'acétate de tributyle-étain, à des concentrations variant de 5 à 100 micro- grammes/l a été étudiée sur les oeufs de Crassostrea gigas en cours de fécondation. Parallèlement les mêmes concentrations ont été testées sur les larves D âgées de 24 heures.
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Expérimentation; Larves; Reproduction; Sel organo-métallique; Acétate de tributyle étain.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/00009/11983/8687.pdf
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Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage ArchiMer
Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf
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An evaluation of Mg/Ca, Sr/Ca, and Ba/Ca ratios as environmental proxies in aragonite bivalve shells ArchiMer
Poulain, C.; Gillikin, D. P.; Thebault, Julien; Munaron, Jean-marie; Bohn, M; Robert, Rene; Paulet, Yves-marie; Lorrain, Anne.
The influence of salinity and water chemistry on Mg/Ca, Sr/Ca and Ba/Ca ratios in the aragonitic shells of the Manila clam were investigated. Clams were reared at constant temperature (20 °C) under different controlled conditions of salinity, commonly encountered in their natural habitat. Clams were held in three tanks with a constant salinity of 35 for the first 35 days and then two tanks were changed to lower salinities (20 and 28) for the next 29 days. Individual shell Mg/Ca, Sr/Ca and Ba/Ca ratios were studied through time. Despite stable conditions (temperature, salinity, and Mg/Cawater) for clams reared at salinity 35 during the experiment, Mg/Ca shell ratios increased through the time. Moreover the salinity decrease at t35 slowed the increase of...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Aragonite; Sclerochronology; Bivalved mollusk; Trace element; Proxy.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00245/35649/34158.pdf
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Anaesthesia and gonad sampling in the European flat oyster (Ostrea edulis) ArchiMer
Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene.
Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the...
Tipo: Text Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf
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Analysis of larval oyster grazing by flow cytometry ArchiMer
Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene.
The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice...
Tipo: Text Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf
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Approche économique de la pêche bonitière en Polynésie française ArchiMer
Ugolini, Bruno; Robert, Rene; Mauri, Moea.
Tipo: Text
Ano: 1983 URL: http://archimer.ifremer.fr/doc/00091/20202/17864.pdf
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Bacterial problems associated with scallop Pecten maximus larval culture ArchiMer
Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique.
Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in...
Tipo: Text Palavras-chave: Larvae; Bivalve; Vibrio; Scallop.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf
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Bilan des essais d'élevage du pétoncle noir, Chlamys vria (Linné) dans le Bassin d'Arcachon France ArchiMer
Robert, Rene; Trut, Gilles; Laborde, Jean-louis; Miner, Philippe.
Until 1960, the black scallop Chlamys varia (Linné, 1758) was largely distributed over the French Atlantic coast. Nowadays, most of the deposits are gone. Since controlled mollusc reproduction techniques are now relatively mastered, some reimplatation attempts have been conducted on a number of oyster culture sites, including the Arcachon Bay, from 1989 to 1992. Despite some good results in hatchery and nursery, the rearing attempts failed. If some good growth rates have been monitored in the Arcachon Bay over these three years, these performances have been frustrated by very high mortality rates occurring shortly after the setting up of the farms. Although this species is known to be relatively sensitive to environmental factors, the hydrobiological...
Tipo: Text Palavras-chave: Bassin Arcachon; Elevage; Chlamys varia; Pétoncle.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-6193.PDF
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Bivalve hatchery technology: The current situation for the Pacific oyster Crassostrea gigas and the scallop Pecten maximus in France ArchiMer
Robert, Rene; Gerard, Andre.
The French oyster farming industry relies almost exclusively on juveniles collected from the natural environment; the supply of spat produced by hatcheries is low, about 10% of the industry's requirements. Development through selective breeding of oyster stocks which are better suited for aquaculture purposes, is likely to reverse this tendency since only hatcheries will be able to supply such animals. Scallop farming, which at present is poorly developed in France, relies exclusively on hatchery produced spat. Although hatchery technology is constantly being improved, significant production problems remain which must be solved before hatcheries become a major supplier of juveniles for the industry. This paper describes the present state of hatchery...
Tipo: Text Palavras-chave: Pecten maximus; Crassostrea gigas; Hatchery; Molluscan rearing; Pecten maximus; Crassostrea gigas; Ecloserie; Élevage de bivalve.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-861.pdf
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Comparaison de deux méthodes de prélèvement pour le dénombrement des larves de Crassostrea gigas en zone conchylicole ArchiMer
His, Edouard; Borel, Michel; Robert, Rene; Laborde, Jean-louis.
La numération des larves d'huître dans le plancton renseigne les ostréiculteurs sur la date de pose des collecteurs. La technique utilisée est très ancienne (BOURY, 1928) ; pour chaque station trois prélèvements par traits de filets pendant 10 minutes sont effectués : deux en surface (filet 200 et filet 130) et un à 1 mètre de profondeur (filet 130). Une méthode par pompage avec mesure instantanée du volume d'eau filtrée a été mise au point (prélèvements en surface et à 1 mètre de profondeur, et rétention des véligères sur filets 200). La comparaison des deux techniques met en évidence l'inadaptation de l'ancienne méthode. Le choix de la porosité (70 µm pour les filets 200) est mal adapté à la récolte des larves D (stade 1) dont le nombre peut être sous...
Tipo: Text Palavras-chave: Numération; Technique; Reproduction; Crassostrea gigas; Larves; Naissain; Recrutement; Ostréiculture.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-6444.PDF
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Competition for food in the larvae of two marine molluscs, Crepidula fornicata and Crassostrea gigas ArchiMer
Blanchard, Michel; Pechenik, J; Giudicelli, E; Connan, Jean-paul; Robert, Rene.
The degree to which larvae of the invasive American slipper limpet (Crepidula fornicata) and the Japanese oyster (Crassostrea gigas) may compete for food was examined during 2003 in the laboratory. Larval microalgae uptake, growth and mortality were compared for larvae fed each of six species of unicellular algae, ranging in length from 2 to 10 mu m. Tested diets included the two flagellates Tetraselmis chui (Prasinophyceae) and Isochrysis affinis galbana (T-ISO, Haptophyceae), one member of the Chlorophyceae (Nannochloris atomus), and three diatom species (Chaetoceros calcitrans forma pumilum, Chaetoceros gracilis, Skeletonema marino). We found that the limpet larvae ingested phytoplankton over a wider range of cell sizes and ate at higher rates on each...
Tipo: Text Palavras-chave: Larvae; Crepidula fornicata; Crassostrea gigas Growth Feeding rate Diet Phytoplankton.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4703.pdf
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Compte-rendu de mission en Tunisie (13-17 Juin 2005). Atelier de travail sur les nouvelles perspectives de la recherche scientifique en aquaculture ArchiMer
Thouard, Emmanuel; Buchet, Vincent; Cahu, Chantal; Coeurdacier, Jean-luc; Elie, Pierre; Gerard, André; Hussenot, Jerome; Legendre, Marc; Prou, Jean; Robert, Rene.
Les missions réalisées fin 2004 et début 2005 ont permis de faire le point sur les coopérations potentielles entre l'INSTM et l'IFREMER. Un certain nombre de domaines de coopération et de sujets de collaboration ont été identifiés et se sont traduits (ou se traduiront) par des actions de coopération (missions, stages, formations) soutenues financièrement par le SCAC de l'Ambassade de France à Tunis. La coopération entre l'IFREMER et l'INSTM dans le domaine de l'aquaculture a été très active dans le cadre du programme« Aquaculture 2001 » (1997-2001). Depuis cette période les relations se sont limitées à la préparation du programme DORAD qui était la suite d' «Aquaculture 2001 » . En l'absence du financement FSP sollicité pour DORAD, les deux organismes ont...
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Ano: 2005 URL: http://archimer.ifremer.fr/doc/00305/41648/40857.pdf
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