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| Registros recuperados: 14 | |
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| Nacry, P.; Canivenc, G.; Muller, B.; Azim, A.; Van Onckelen, H.; Rossignol, M.; Doumas, P.. |
| The changes in root system architecture (RSA) triggered by phosphate (P) deprivation were studied in Arabidopsis (Arabidopsis thaliana) plants grown for 14 d on 1 mM or 3 mM P. Two different temporal phases were observed in the response of RSA to low P. First, lateral root (LR) development was promoted between days 7 and 11 after germination, but, after day 11, all root growth parameters were negatively affected, leading to a general reduction of primary root ( PR) and LR lengths and of LR density. Low P availability had contrasting effects on various stages of LR development, with a marked inhibition of primordia initiation but a strong stimulation of activation of the initiated primordia. The involvement of auxin signaling in these morphological changes... |
| Tipo: Journal Article |
Palavras-chave: CARENCE EN PHOSPHATE; CROISSANCE RACINAIRE; CONTROLE HORMONAL; MORPHOLOGIE RACINAIRE PHOSPHORUS DEFICIENCY. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007df2073ce&uri=/notices/prodinra1/2007/02/ |
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| Dahan, J.; Pichereaux, C.; Rossignol, M.; Blanc, S.; Wendehenne, D.; Pugin, A.; Bourque, S.. |
| When a plant cell is challenged by a well-defined stimulus, complex signal transduction pathways are activated to promote the modulation of specific sets of genes and eventually to develop adaptive responses. In this context, protein phosphorylation plays a fundamental role through the activation of multiple protein kinase families. Although the involvement of protein kinases at the plasma membrane and cytosolic levels are now well-documented, their nuclear counterparts are still poorly investigated. In the field of plant defence reactions, no known study has yet reported the activation of a nuclear protein kinase and/or its nuclear activity in plant cells, although some protein kinases, e.g. MAPK (mitogen-activated protein kinase), are known to be... |
| Tipo: Journal Article |
Palavras-chave: PLANTE; CRYPTOGEINE; ELICITEUR CRYTOGEIN; PLANT DEFENCE; PROTEIN KINASE; SALICYLIC ACID-INDUCED PROTEIN KINASE (SIPK); TOBACCO CELL. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011b44b44d&uri=/notices/prodinra1/2011/05/ |
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| Chevalier, F.; Centeno, D.; Rofidal, V.; Tauzin, M.; Martin, O.; Sommerer, N.; Rossignol, M.. |
| Replicate 2-D gels were stained with four visible or fluorescent dyes using published procedures, and48 co-detected spots were selected for contrasting values in abundance, Mr and p I. Success rate ofidentification and sequence coverage were affected in a dye-dependent manner by the three parameters.Frequency of missed cleavages and recovery of sulfur-containing peptides also depended on the dye.Finally, the dataset was used to predict the number of proteins identifiable when integrating thedifferential contribution of each parameter. Sypro Ruby appeared to combine several favorablefeatures: no dependence of the identification rate upon the physicochemical properties of proteins,no impact on frequency of missed cleavages, and a higher predicted... |
| Tipo: Journal Article |
Palavras-chave: VISIBLE STAINING; FLUORESCENT DYES; PEPTIDE MASS FINGERPRINT; PROTEIN IDENTIFICATION; SEQUENCE COVERAGE; PROTEIN PROPERTIES; MISSED CLEAVAGES. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200855bb4aa4&uri=/notices/prodinra1/2008/04/ |
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| Nten, C.M.A.; Sommerer, N.; Rofidal, V.; Hirtz, C.; Rossignol, M.; Cuny, G.; Peltier, J.B.; Geiger, A.. |
| Trypanosoma secretome was shown to be involved in parasite virulence and is suspected of interfering in parasite life-cycle steps such as establishment in the Glossina midgut, metacyclogenesis. Therefore, we attempted to identify the proteins secreted by procyclic strains of T. brucei gambiense and T. brucei brucei, responsible for human and animal trypanosomiasis, respectively. Using mass spectrometry, 427 and 483 nonredundant proteins were characterized in T. brucei brucei and T. brucei gambiense secretomes, respectively; 35% and 42% of the corresponding secretome proteins were specifically secreted by T. brucei brucei and T. brucei gambiense, respectively, while 279 proteins were common to both subspecies. The proteins were assigned to 12 functional... |
| Tipo: Journal Article |
Palavras-chave: TRYPANOSOME; SECRETOME; TRANSMISSION; MOUCHE TSE-TSE. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201013996fd2&uri=/notices/prodinra1/2010/08/ |
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| Geiger, A.; Hirtz, C.; Becue, T.; Bellard, E.; Centeno, D.; Gargani, D.; Rossignol, M.; Cuny, G.; Peltier, J.B.. |
| Background: Human African trypanosomiasis is a lethal disease caused by the extracellular parasite Trypanosoma brucei. The proteins secreted by T. brucei inhibit the maturation of dendritic cells and their ability to induce lymphocytic allogenic responses. To better understand the pathogenic process, we combined different approaches to characterize these secreted proteins.Results: Overall, 444 proteins were identified using mass spectrometry, the largest parasite secretome described to date. Functional analysis of these proteins revealed a strong bias toward folding and degradation processes and to a lesser extent toward nucleotide metabolism. These features were shared by different strains of T. brucei, but distinguished the secretome from published T.... |
| Tipo: Journal Article |
Palavras-chave: TRYPANOSOME; SPECTROMETRIE DE MASSE. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20106a992200&uri=/notices/prodinra1/2010/08/ |
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| Prak, S.; Hem, S.; Boudet, J.; Viennois, G.; Sommerer, N.; Rossignol, M.; Maurel, C.; Santoni, V.. |
| Aquaporins form a family of water and solute channel proteins and are present in most living organisms. In plants, aquaporins play an important role in the regulation of root water transport in response to abiotic stresses. In this work, we investigated the role of phosphorylation of Plasma membrane Intrinsic Protein (PIP) aquaporins in the Arabidopsis thaliana root by a combination of quantitative mass spectrometry and cellular biology approaches. A novel phosphoproteomic procedure that involves plasma membrane purification, phosphopeptide enrichment with TiO2 columns, and systematic mass spectrometry sequencing revealed multiple and adjacent phosphorylation sites in the C-terminal tail of several AtPIPs. Six of these sites had not been previously... |
| Tipo: Journal Article |
Palavras-chave: AQUAPORIN; CHANNEL PROTEINS; WATER TRANSPORT; PLASMA MEMBRANE; SALT STRESS. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009724b97eb&uri=/notices/prodinra1/2010/09/ |
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| Hem, S.; Rofidal, V.; Sommerer, N.; Rossignol, M.. |
| The generation of novel subsets of phosphorylation sites is needed to complement the present Arabidopsis plasma membrane phosphoproteinrepertoire, where several families of proteins are under-represented. In this work, different combinations of chromatographicsteps were first compared for capacity to resolve model phosphopeptides before characterisation from PSD fragments in MALDI MS/MS. Nearly half of the phosphorylation sites detected in the Arabidopsis plasmalemma using the optimised procedure were novel, andtwo-thirds of protein accessions identified secondary active transporters. These included phosphate/H+ symporters, ammonium andnitrate transporters, different alkali cation exchangers, a urea/H+ symporter, a glucose transporter, a purine permease,... |
| Tipo: Journal Article |
Palavras-chave: ARABIDOPSIS; PLASMA MEMBRANE; SOS1; PHOSPHOPEPTIDE; SECONDARY ACTIVE TRANSPORTER; AMT1.1; CHX17; DUR3; PHT1. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200861196512&uri=/notices/prodinra1/2008/05/ |
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| Stanislas, T.; Bouyssie, D.; Rossignol, M.; Vesa, S.M.; Fromentin, J.; Morel, J.; Pichereaux, C.; Plas Simon, F.; Monsarrat, B.. |
| A large body of evidence from the past decade supports the existence, in membrane from animal and yeast cells, of functional microdomains playing important roles in protein sorting, signal transduction, or infection by pathogens. In plants, as previously observed for animal microdomains, detergent-resistant fractions, enriched in sphingolipids and sterols, were isolated from plasma membrane. A characterization of their proteic content revealed their enrichment in proteins involved in signaling and response to biotic and abiotic stress and cell trafficking suggesting that these domains were likely to be involved in such physiological processes. In the present study, we used 14N/15N metabolic labeling to compare, using a global quantitative proteomics... |
| Tipo: Journal Article |
Palavras-chave: ELICITEUR. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011b4dcb8b0&uri=/notices/prodinra1/2011/05/ |
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| Holzmuller, P.; Grébaut, P.; Peltier, J.B.; Brizard, J.P.; Perrone, T.; Gonzatti, M.; Bengaly, Z.; Rossignol, M.; Aso, P.M.; Vincendeau, P.; Cuny, G.; Boulangé, A.; Frutos, R.. |
| Animal trypanosomosis is one of the most severe constraints to agricultural development in sub-Saharan Africa and is also an important disease of livestock in Latin America and Asia. The causative agents are various species of protozoan parasites belonging to the genus Trypanosoma, among which T. congolense and T. evansi are the major pathogenic species. The extracellular position of trypanosomes obliges us to consider both the parasite and its excreted/secreted factors in the course of the physiopathologic process. The advent of proteomics led us to propose a comparative approach of the proteome (i.e., the whole parasite content) and the secretome (i.e., naturally excreted/secreted molecules) of T. congolense and T. evansi with particular attention to... |
| Tipo: Conference Paper |
Palavras-chave: ANIMAL TRYPANOSOMOSIS; SECRETOME; VIRULENCE; PATHOGENICITY; PROTEOMICS; DIAGNOSIS; DRUG TARGETS. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201021915a35&uri=/notices/prodinra1/2010/08/ |
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| Rossignol, M.. |
| The Ebridians, marine planktonic flagellates with an internal siliceous skeleton, used to be very numerous in the Tertiary era but are only represented nowadays by two genera: "Ebria" Borgert (1891) and "Hermesinum" Zacharias (1906). Found successively in the Baltic Sea, the North Sea, the Mediterranean Sea, the northern Pacific Ocean, the northern and southern Atlantic Ocean, they had never been observed in tropical seas. Consequently, they were considered as cold or temperate water organisms. Their presence on the western African coast, at latitude 4° 48' S, in addition to extending their distribution area, seems to invalidate this point of view. Without drawing any hasty conclusion, it seemed interesting to recall their appearance in the Bay of... |
| Tipo: Text |
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| Ano: 1956 |
URL: http://archimer.ifremer.fr/doc/1956/publication-4679.pdf |
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| Ky, C.L.; de Lorgeril, J.; Hirtz, C.; Sommerer, N.; Rossignol, M.; Bonhomme, F.. |
| The European sea bass, Dicentrarchus labrax L., tolerates a range of salinities from freshwater to hyper-saline. To study differences in protein expression, fish were reared in both freshwater and seawater. After 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. In all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. Fifty differential protein spots were excised from a colloidal Coomassie-stained gel. Nine separate protein spots were identified unambiguously by mass spectrometry and database searching. Among the six proteins over-expressed in gill cells in seawater, five were cytoskeleton proteins and one was the aromatase... |
| Tipo: Journal Article |
Palavras-chave: MALDI-TOF MASS SPECTROMETRY; REAL TIME PCR; SALINITY; SEA BASS; TWO-DIMENSIONAL GEL ELECTROPHORESIS. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008f070b07f&uri=/notices/prodinra1/2008/04/ |
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| Delom, F.; Szponarski, W.; Sommerer, N.; Boyer, J.C.; Bruneau, J.M.; Rossignol, M.; Gibrat, R.. |
| Calcofluor is an antifungal compound known to induce structural perturbations of the cell wall by interfering with the synthesis of chitin microfibril. Proteins from a stripped plasma membrane fraction were solubilized with the neutral and non-denaturing detergent, the n-dodecyl beta-D-maltoside. Proteins were then resolved using a recently described ion-exchange chromatography (IEC)/lithium dodecyl sulfate (LDS)-PAGE procedure. Nearly 90 proteins were identified and clustered, based on their pI, molecular weight, abundance and/or hydrophobicity. This method was then applied to profile the plasma membrane response to calcofluor. The LDS-PAGE patterns obtained from whole plasma membrane proteins were similar for the non-treated and calcofluor-treated... |
| Tipo: Journal Article |
Palavras-chave: PROTEINE MEMBRANAIRE CELL WALL; LIQUID CHROMATOGRAPHY; PLASMA MEMBRANE PROTEINS; SACCHAROMYCES CEREVISIAE. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20072157644e&uri=/notices/prodinra1/2007/04/ |
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| Registros recuperados: 14 | |
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