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Registros recuperados: 13
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Changes in Atlantic cod (Gadus morhua L.) sperm quality during the spawning season ArchiMer
Rouxel, Catherine; Suquet, Marc; Cosson, J; Severe, Armelle; Quemener, Loic; Fauvel, Christian.
The biology of cod reproduction is well described in the scientific literature. However, sperm biology and spermatozoa management are poorly studied in this species. Because of its recent farming expansion, a better knowledge of cod gametes is becoming especially useful. This work aimed at establishing tools to study sperm biology in cod, and also investigated the existence of changes in cod sperm quality during the spawning period. We showed that sperm concentration could be assessed using spectrophotometry at 260 nm. Sperm motility significantly decreased after a 168-h storage at 4 degrees C. A 1:9 dilution of sperm in a non-activating medium (1/3 seawater and 2/3 freshwater, osmotic pressure: 360 mOsm kg(-1)) improved sperm storage. Sperm concentration,...
Tipo: Text Palavras-chave: Spermatozoa; Quality; Ageing; Sperm; Cod; Gadus morhua.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3829.pdf
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Conservation par le froid du patrimoine génétique de l'huÎtre perlière Pinctada margaritifera - Rapport final de la Convention n° 40408 Service de la perliculture - Ifremer ArchiMer
Vonau, Vincent; Matehau, Ariora; Rouxel, Catherine; Cochard, Jean-claude.
La congélation du sperme et des embryons d'huîtres perlières est un moyen de renforcer la sécurité du potentiel économique que représente la Perliculture en Polynésie Française. Elle permet la conservation, à faible coût, de la biodiversité ex-situ des populations d'huîtres perlières sauvages et des génotypes de sujets présentant un intérêt particulier pour l'industrie de la perle. Un avantage complémentaire de la cryoconservation sera de faciliter l'avancement des programmes d'amélioration génétique. À ce jour, les divers travaux réalisés sur les gamètes des mollusques bivalves ont permis de congeler la laitance de différentes espèces autres que Pinctada margaritifera. Cependant, quelle que soit l'espèce, le protocole complet n'a pas été validé pour...
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Ano: 2005 URL: http://archimer.ifremer.fr/doc/00304/41501/40709.pdf
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Draft genomes and phenotypic characterization of Tisochrysis lutea strains. Toward the production of domesticated strains with high added value ArchiMer
Carrier, Gregory; Baroukh, Caroline; Rouxel, Catherine; Duboscq-bidot, Laetitia; Schreiber, Nathalie; Bougaran, Gael.
Tisochrysis lutea is a microalga species currently used in aquaculture as a feed for shellfish, oysters and shrimps. It also has many other potential industrial applications, such as the production of neutral lipids for biofuels or the production of ω-3 fatty acids for nutraceuticals (human food complements). To efficiently exploit the potential of this microalga, however, higher lipid productivities are needed. To this end, improvement programs need to be developed and optimized. The diversity of strains available in microalgae has not yet been exploited in such improvement programs. In this study, the intra-strain diversity was observed and exploited to increase neutral lipid productivity. New clonal strains with higher neutral lipid productivity were...
Tipo: Text Palavras-chave: Microalgae; Genome; Improvement program; Lipids; Tisochrysis lutea.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00411/52204/52927.pdf
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Effects of blue light on the biochemical composition and photosynthetic activity of Isochrysis sp. (T-iso) ArchiMer
Marchetti, Julie; Bougaran, Gael; Jauffrais, Thierry; Lefebvre, Sebastien; Rouxel, Catherine; Saint-jean, Bruno; Lukomska, Ewa; Robert, Rene; Cadoret, Jean-paul.
In aquaculture, particularly in bivalve hatcheries, the biochemical composition of algal diets has a strong influence on larval and post-larval development. Biochemical composition is known to be related to culture conditions, among which light represents a major source of variation. The effects of blue light on biochemical composition and photosynthetic rate of Isochrysis sp. (T-iso) CCAP 927/14 were assessed in chemostat at a single irradiance (300 μmol photons m−2 s−1) and compared with white light. Two different dilution (renewal) rates were also tested: 0.7 and 0.2 d−1. Relative carbohydrate content was lower under blue light than under white light at both dilution rates, whereas chlorophyll a and photosynthesis activity were higher. In contrast,...
Tipo: Text Palavras-chave: Isochrysis; Blue light; Photosynthesis; Proximate composition; Chemostat.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00085/19599/17237.pdf
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Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure ArchiMer
Bougaran, Gael; Rouxel, Catherine; Dubois, Nolwenn; Kaas, Raymond; Grouas, Sophie; Lukomska, Ewa; Le Coz, Jean-rene; Cadoret, Jean-paul.
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
Tipo: Text Palavras-chave: Strain selection; Isochrysis; Neutral lipid; Ultra violet; Flux cytometry; Biofuel.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00085/19643/17418.pdf
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Genetic structure at different spatial scales in the pearl oyster (Pinctada margaritifera cumingii) in French Polynesian lagoons: beware of sampling strategy and genetic patchiness ArchiMer
Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre.
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat...
Tipo: Text Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf
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Génotypage des géniteurs de Lates calcarifer de Tahiti : aide à la domestication raisonnée du Loup Tropical pour la filière tahitienne ArchiMer
Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel.
The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder...
Tipo: Text Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf
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High-affinity nitrate/nitrite transporter genes (Nrt2) in Tisochrysis lutea: identification and expression analyses reveal some interesting specificities of Haptophyta microalgae ArchiMer
Charrier, Aurelie; Berard, Jean-baptiste; Bougaran, Gael; Carrier, Gregory; Lukomska, Ewa; Schreiber, Nathalie; Fournier, Flora; Rouxel, Catherine; Garnier, Matthieu; Cadoret, Jean-paul; Saint-jean, Bruno.
Microalgae have a diversity of industrial applications such as feed, food ingredients, depuration processes and energy. However, microalgal production costs could be substantially improved by controlling nutrient intake. Accordingly, a better understanding of microalgal nitrogen metabolism is essential. Using in silico analysis from transcriptomic data concerning the microalgae Tisochrysis lutea, four genes encoding putative high-affinity nitrate/nitrite transporters (TlNrt2) were identified. Unlike most of the land plants and microalgae, cloning of genomic sequences and their alignment with complementary DNA (cDNA) sequences did not reveal the presence of introns in all TlNrt2 genes. The deduced TlNRT2 protein sequences showed similarities to NRT2...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00253/36467/35044.pdf
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Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition ArchiMer
Da Costa, F.; Petton, Bruno; Mingant, Christian; Bougaran, Gael; Rouxel, Catherine; Quere, Claudie; Wikfors, G. H.; Soudant, P.; Robert, Rene.
Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate...
Tipo: Text Palavras-chave: Larvae; Lipids; Oyster; Tisochrysis lutea.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00251/36258/36063.pdf
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Le contrôle des croisements chez l’huître perlière Pinctada margaritifera : La maturation ovocytaire in vitro et l’induction de la ponte ArchiMer
Le Moullac, Gilles; Rouxel, Catherine; Vonau, Vincent; Ledu, Christophe; Cochard, Jean-claude.
Afin de mener à bien le programme Maîtrise de la Reproduction chez l’huître perlière Pinctada margaritifera dont la perspective est l’amélioration génétique, il est essentiel de contrôler les croisements au niveau individuel. Ces croisements ne pourront être contrôlés que dans la mesure où les gamètes mâles et femelles sont obtenus séparément. Ceci permettra aussi de valider les travaux en cryoconservation des spermatozoïdes et de faciliter la production de polyploïdes. Ces travaux répondent aussi aux préoccupations d’amélioration génétique. Cette étude est basée sur deux approches complémentaires. D’une part, l’étude de la maturation ovocytaire in vitro afin de déterminer les conditions qui permettront de disposer d’ovocytes prêts à être fécondés. D’autre...
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Ano: 2003 URL: http://archimer.ifremer.fr/doc/00182/29290/27731.pdf
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Observation of the larval distribution of two soleidae: Solea solea (L.) and Microchirus variegatus (Donovan) in the Bay of Biscay ArchiMer
Lacroix, Nicole; Rouxel, Catherine; Dorel, Didier.
Surveys for larval stages of Dover sole (Solea solea) and Thickback sole (Microchirus variegatus) were carried out in the northern part of the bay of Biscay between 1985 and 1989. The spawning periods of these species overlap but the spawning areas are different since young larvae of M. variegatus are recorded more offshore than those of S. solea. In addition, in spite of a similar behaviour the first species undergo smaller vertical migrations than the second and never reach surface layers. These two observations suggest why Thickhack sole larvae on the contrary of Dover sole larvae never reach coastal areas and achieve whole development in water deeper than 40 m. The life cycle of this species developing thus entirely offshore.
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/00394/50571/51262.pdf
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The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae ArchiMer
Rumin, Judith; Bonnefond, Hubert; Saint-jean, Bruno; Rouxel, Catherine; Sciandra, Antoine; Bernard, Olivier; Cadoret, Jean-paul; Bougaran, Gael.
Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content,...
Tipo: Text Palavras-chave: Nile red; BODIPY 505/515; Microalgae; Neutral lipid; Fluorescence; Biodiesel.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00257/36823/35451.pdf
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Use of a lipid rich strain reveals mechanisms of nitrogen limitation and carbon partitioning in the haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno.
Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied...
Tipo: Text Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00356/46702/46904.pdf
Registros recuperados: 13
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