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Lima,Laisy Garcia Ribeiro; Gonçalves,Márcia Monteiro Machado; Couri,Sonia; Melo,Verônica Ferreira; Sant’Ana,Gizele Cardoso Fontes; Costa,Antônio Carlos Augusto da. |
Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Submerged fermentation; Aspergillus nige; Experimental design.. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100214 |
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