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Registros recuperados: 36
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A new potentially toxic Azadinium species (Dinophyceae) from the Mediterranean Sea, A. dexteroporum sp. nov. ArchiMer
Percopo, Isabella; Siano, Raffaele; Rossi, Rachele; Soprano, Vittorio; Sarno, Diana; Zingone, Adriana.
A new photosynthetic planktonic marine dinoflagellate, Azadinium dexteroporum sp. nov., is described from the Gulf of Naples (South Tyrrhenian Sea, Mediterranean Sea). The plate formula of the species, Po, cp, X, 4, 3a, 6, 6C, 5?S, 6'" and 2, is typical for this recently described genus. Azadinium dexteroporum is the smallest rep-resentative of the genus (8.5m average length, 6.2m average width) and shares the presence of a small antapical spine with the type species A.spinosum and with A.polongum. However, it differs from all other Azadinium species for the markedly asymmetrical Po plate and the position of the ventral pore, which is located at the right posterior end of the Po plate. Another peculiarity of A.dexteroporum is the pronounced concavity of...
Tipo: Text Palavras-chave: Azadinium; Azaspiracid; Dinoflagellate; LC; MS-TOF; LTER-MC; Mediterranean Sea; Phylogeny; Taxonomy.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28199/27278.pdf
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Acquisition de données complémentaires aux dénombrements, avec les techniques d’analyses pigmentaires et de diversité génétique. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° AIV. Rapport final, 16 juillet 2014. ArchiMer
Delmas, Daniel; Siano, Raffaele.
Studies on phytoplankton community diversity cannot be limited microphytoplankton (cells>20 ìm), it is indeed necessary to take into account also the nano (3-20ìm) picophytoplankton (<3 ìm) components. In the frame of this work aimed at studying temporal phytoplankton community diversity two complementary methodologies of phytoplankton sample analysis, pigment and genetic (metarbarcoding) diversity, have been used as alternative to routine morphological analysis carried out at light microscopy. The REPHY station (047-P-016 Concarneau Large) off-shore the Concarneau Bay has been sampled during the annual highest phytoplankton production (March-July 2012) with a frequent sample interval strategy (twice per week). Results obtained by both pigment and...
Tipo: Text Palavras-chave: Phytoplancton; Structure de taille; Picophytoplancton; Nanophytoplancton; Microphytoplancton; Diversité génétique; Metabarcoding; Taxonomie pigmentaire; Façade atlantique; Baie de Concarneau; Phytoplankton; Size classes; Picophytoplankton; Nanophytoplankton; Microphytoplankton; Genetic diversity; Metabarcoding; Pigment taxonomy; Atlantic coasts; Concarneau Bay.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47260/47225.pdf
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Analysis of the genomic basis of functional diversity in dinoflagellates using a transcriptome-based sequence similarity network ArchiMer
Meng, Arnaud; Corre, Erwan; Probert, Ian; Gutierrez-rodriguez, Andres; Siano, Raffaele; Annamale, Anita; Alberti, Adriana; Da Silva, Corinne; Wincker, Patrick; Le Crom, Stephane; Not, Fabrice; Bittner, Lucie.
Dinoflagellates are one of the most abundant and functionally diverse groups of eukaryotes. Despite an overall scarcity of genomic information for dinoflagellates, constantly emerging high-throughput sequencing resources can be used to characterize and compare these organisms. We assembled de novo and processed 46 dinoflagellate transcriptomes and used a sequence similarity network (SSN) to compare the underlying genomic basis of functional features within the group. This approach constitutes the most comprehensive picture to date of the genomic potential of dinoflagellates. A core-predicted proteome composed of 252 connected components (CCs) of putative conserved protein domains (pCDs) was identified. Of these, 206 were novel and 16 lacked any functional...
Tipo: Text Palavras-chave: Genomics; Proteomics; Microbial biology; Molecular evolution; Protists; Transcriptomics.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00444/55550/57209.pdf
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Benthic protists: the under-charted majority ArchiMer
Forster, Dominik; Dunthorn, Micah; Mahe, Frederic; Dolan, John R.; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Edvardsen, Bente; Egge, Elianne; Eikrem, Wenche; Gobet, Angelique; Kooistra, Wiebe H. C. F.; Logares, Ramiro; Massana, Ramon; Montresor, Marina; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Romac, Sarah; Shalchian-tabrizi, Kamran; Simon, Nathalie; Richards, Thomas A.; Santini, Sebastien; Sarno, Diana; Siano, Raffaele; Vaulot, Daniel; Wincker, Patrick; Zingone, Adriana; De Vargas, Colomban; Stoeck, Thorsten.
Marine protist diversity inventories have largely focused on planktonic environments, while benthic protists have received relatively little attention. We therefore hypothesize that current diversity surveys have only skimmed the surface of protist diversity in marine sediments, which may harbour greater diversity than planktonic environments. We tested this by analyzing sequences of the hypervariable V4 18S rRNA from benthic and planktonic protist communities sampled in European coastal regions. Despite a similar number of OTUs in both realms, richness estimations indicated that we recovered at least 70% of the diversity in planktonic protist communities, but only 33% in benthic communities. There was also little overlap of OTUs between planktonic and...
Tipo: Text Palavras-chave: Benthic microbial communities; Coastal environments; Protist diversity; High-throughput sequencing.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00341/45233/44658.pdf
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Coupling between taxonomic and functional diversity in protistan coastal communities ArchiMer
Ramond, Pierre; Sourisseau, Marc; Simon, Nathalie; Romac, Sarah; Schmitt, Sophie; Rigaut-jalabert, Fabienne; Henry, Nicolas; De Vargas, Colomban; Siano, Raffaele.
The study of protistan functional diversity is crucial to understand the dynamics of oceanic ecological processes. We combined the metabarcoding data of various coastal ecosystems and a newly developed trait‐based approach to study the link between taxonomic and functional diversity across marine protistan communities of different size‐classes. Environmental DNA was extracted and the V4 18S rDNA genomic region was amplified and sequenced. In parallel, we tried to annotate the Operational Taxonomic Units (OTUs) from our metabarcoding dataset to 30 biological traits using published and accessible information on protists. We then developed a method to study trait correlations across protists (i.e. trade‐offs) in order to build the best functional groups....
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00478/58964/61554.pdf
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Définition d’un indice composition pour le phytoplancton en Manche- Atlantique, à partir des données du micro-phytoplancton du REPHY et des réseaux régionaux, et des mesures complémentaires de la flore phytoplanctonique acquises avec des techniques novatrices. Action 3 – Livrable 1. Propositions pour un indice de composition du phytoplancton, basé sur les résultats des méthodes microscopie, pigments et diversité génétique. Rapport final, février 2016 ArchiMer
Hernandez Farinas, Tania; Brun, Melanie; Siano, Raffaele; Delmas, Daniel.
This deliverable is composed of two reports which propose indice of phytoplankton composition, based respectively on the results of pigment and genetic diversity methods, and the results of microscopy method. Proposals based on the results of pigment and genetic diversity methods. The present work is a continuation of several studies carried out in the past (Siano and Delmas, 2013, 2015) on investigating an indicator of phytoplankton diversity using two methods complementary to optical microscopy: the genetic diversity analyzed with metabarcoding (exhaustive analysis of the diversity and relative abundance of OTUs, i.e. groups of sequences identified by their genetic resemblance) and the pigmentary diversity (analysis of the diversity and abundance of...
Tipo: Text Palavras-chave: Metabarcoding; OTUs; Pigments; Picophytoplancton; Indice composition; Niche ecologique; Mamiellophyceae; Chlorophylle b; Rade de Brest; Bretagne sud; Panache de la Gironde; Denombrements du micro-phytoplancton; Abondance totale; Monotonicite; Linearite; Pressions; Facade Manche-Atlantique; Metabarcoding; OTUs; Pigments; Picophytoplankton; Composition indice; Ecological niche; Mamiellophyceae; Chlorophyll b; Brest Bay; Southern Britain; Gironde plume; Micro-phytoplankton counts; Total abundance; Monotonicity; Linearity; Pressure; Channel-Atlantic coast.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00363/47421/47426.pdf
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Dinoflagellate fossils: Geological and biological applications ArchiMer
Penaud, Aurelie; Hardy, William; Lambert, Clement; Marret, Fabienne; Masure, Edwige; Seryais, Thomas; Siano, Raffaele; Wary, Melanie; Mertens, Kenneth.
Dinoflagellates are part of the marine plankton and about 200 species produce a cyst (dinocyst) during their life cycle, these organic-walled sexually-produced cysts being fossilizable in sediments for hundreds of millions of years. Over the past 40–50 years, dinocysts have led to major advances on Mesozoic-Cenozoic research, in terms of biostratigraphy and paleogeogeography. Dinocyst taxonomy has then been continuously revised, with the tabulation being the main morphological link between living dinoflagellates and fossilized cysts. Over the Quaternary, and based on the principle of uniformitarianism (i.e. species ecology did not change through time), relationships between modern assemblages and present-day environmental factors controlling their...
Tipo: Text Palavras-chave: Dinoflagellate; Cyst-motile stage relationship; Modern dinocyst distribution; Paleoecology; Biostratigraphy; Ancient DNA.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00463/57476/59749.pdf
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Distribution and host diversity of Amoebophryidae parasites across oligotrophic waters of the Mediterranean Sea ArchiMer
Siano, Raffaele; Alves-de-souza, C.; Foulon, E.; Bendif, El Mahdi; Simon, N.; Guillou, L.; Not, F..
Sequences affiliated to Syndiniales (Marine alveolate, MALV) regularly dominate 18S rDNA genetic libraries of nearly all marine ecosystems investigated so far. Among them, Amoebophryidae (MALV group II) is composed of numerous and genetically distant environmental sequences, where Amoebophrya is the only known and formally described genus. Amoebophrya species include virulent pathogens for a wide range of dinoflagellate species. Beside their regular occurrence in marine ecosystems, their quantitative distribution and the environmental factors triggering host infection have barely been studied in open oligotrophic waters. In order to understand the functional role of these parasites in natural environments, we studied the distribution and contribution to...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00026/13719/10824.pdf
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Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer ArchiMer
Balzano, S.; Gourvil, P.; Siano, Raffaele; Chanoine, M.; Marie, D.; Lessard, S.; Sarno, D.; Vaulot, Daniel.
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00114/22491/20172.pdf
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DYNAPSE - DYNamiques des efflorescences et de la toxicité des espèces phytoplanctoniques nuisibles du genre PSEudo nitzschia en région Loire Bretagne ArchiMer
Belin, Catherine; Chapelle, Annie; Delmas, Daniel; Nezan, Elisabeth; Siano, Raffaele.
Les efflorescences de la diatomée Pseudo-nitzschia sont largement répandues sur les côtes Manche-Atlantique depuis une trentaine d’années (source Rephy). Depuis les années 2000 elles y sont parfois associées des événements toxiques du type ASP (Amnesic Shellfish Poisonning) qui perturbent grandement les pêcheries de coquillages et notamment celles de coquilles Saint Jacques. Les efflorescences et toxicités liées aux Pseudo-nitzschia sont des phénomènes en augmentation sur les côtes françaises mais aussi mondialement. Le genre Pseudo-nitzschia comprend plusieurs espèces très difficiles à identifier en routine or certaines sont toxiques et d’autres pas. Les facteurs environnementaux abiotiques et biotiques contrôlent à la fois la croissance des microalgues...
Tipo: Text Palavras-chave: Algues toxiques; Pseudo nitzschia; Toxicité des coquillages; Pêcheries; Facteurs environnementaux.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00189/30035/28521.pdf
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Étude sur la prolifération de la micro algue Alexandrium minutum en rade de Brest ArchiMer
Chapelle, Annie; Le Bec, Claude; Amzil, Zouher; Dreanno, Catherine; Guillou, Laure; Klouch, Khadidja; Labry, Claire; Le Gac, Mickael; Pineau-guillou, Lucia; Siano, Raffaele; Abernot, Chantal; Andrieux, Francoise; Caradec, Florian; Destombe, Christophe; Dia, A.; Doner, Anne; Duval, Julia; Gouriou, Jeremie; Lazure, Pascal; Le Brun, Luc; Le Gal, Dominique; Malestroit, Pascale; Petton, Sebastien; Plus, Martin; Quere, Julien; Savar, Veronique; Schmitt, Sophie; Terre, A.; Youenou, Agnes.
Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été élaboré avec la Région Bretagne dans le cadre du développement du port de Brest. L’Ifremer a organisé un échantillonnage dans l’estuaire de la rivière de Daoulas, zone la plus touchée en 2012, au cours des printemps-été 2013 et 2014. Un suivi de la présence d’A. minutum dans les sédiments a également été réalisé en hiver 2014. De ces suivis, des mesures réalisées au laboratoire à ce jour, des simulations du modèle Prévimer ainsi que des résultats du réseau Rephy et Velyger, il ressort que: * La rade de Brest peut aujourd’hui être considérée comme une zone à risque pour Alexandrium minutum. En effet, depuis 2012...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00254/36487/35029.pdf
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Étude sur la prolifération de la micro algue Alexandrium minutum en rade de Brest. Projet Daoulex. Rapport d’avancement n° 2 : Analyse des traces biologiques d’Alexandrium minutum dans les sédiments de la Rade de Brest. ArchiMer
Chapelle, Annie; Le Bec, Claude; Siano, Raffaele; Caradec, Florian; Andrieux, Francoise.
Suite à l’efflorescence de grande ampleur d’Alexandrium minutum et des événements toxiques associés en Rade de Brest en 2012, un projet a été monté avec la Région dans le cadre du développement du port de Brest, pour élucider les contrôles de cette efflorescence. De juillet à août 2013, l’Ifremer a organisé un échantillonnage serré dans l’estuaire de Daoulas, zone la plus touchée en 2012 (rapport d’avancement n°1). Une autre partie de ce projet vise à la détection (rapport d’avancement n°2) et à la quantification (rapport d’avancement n°3) des traces biologiques d’A. minutum dans les sédiments de surface en différentes stations de la Rade de Brest présentant des caractéristiques sédimentaires différentes. Pour la détection des traces biologiques (ADN...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00191/30231/28647.pdf
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Eukaryotic plankton diversity in the sunlit ocean ArchiMer
De Vargas, Colomban; Audic, Stephane; Henry, Nicolas; Decelle, Johan; Mahe, Frederic; Logares, Ramiro; Lara, Enrique; Berney, Cedric; Le Bescot, Noan; Probert, Ian; Carmichael, Margaux; Poulain, Julie; Romac, Sarah; Colin, Sebastien; Aury, Jean-marc; Bittner, Lucie; Chaffron, Samuel; Dunthorn, Micah; Engelen, Stefan; Flegontova, Olga; Guidi, Lionel; Horak, Ales; Jaillon, Olivier; Lima-mendez, Gipsi; Lukes, Julius; Malviya, Shruti; Morard, Raphael; Mulot, Matthieu; Scalco, Eleonora; Siano, Raffaele; Vincent, Flora; Zingone, Adriana; Dimier, Celine; Picheral, Marc; Searson, Sarah; Kandels-lewis, Stefanie; Acinas, Silvia G.; Bork, Peer; Bowler, Chris; Gorsky, Gabriel; Grimsley, Nigel; Hingamp, Pascal; Iudicone, Daniele; Not, Fabrice; Ogata, Hiroyuki; Pesant, Stephane; Raes, Jeroen; Sieracki, Michael E.; Speich, Sabrina; Stemmann, Lars; Sunagawa, Shinichi; Weissenbach, Jean; Wincker, Patrick; Karsenti, Eric.
Marine plankton support global biological and geochemical processes. Surveys of their biodiversity have hitherto been geographically restricted and have not accounted for the full range of plankton size. We assessed eukaryotic diversity from 334 size-fractionated photic-zone plankton communities collected across tropical and temperate oceans during the circumglobal Tara Oceans expedition. We analyzed 18S ribosomal DNA sequences across the intermediate plankton-size spectrum from the smallest unicellular eukaryotes (protists, > 0.8 micrometers) to small animals of a few millimeters. Eukaryotic ribosomal diversity saturated at similar to 150,000 operational taxonomic units, about one-third of which could not be assigned to known eukaryotic groups....
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00270/38135/37217.pdf
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Evolutionary processes and cellular functions underlying divergence in Alexandrium minutum ArchiMer
Le Gac, Mickael; Metegnier, Gabriel; Chomerat, Nicolas; Malestroit, Pascale; Quere, Julien; Bouchez, Olivier; Siano, Raffaele; Destombe, Christophe; Guillou, Laure; Chapelle, Annie.
Understanding divergence in the highly dispersive and seemingly homogeneous pelagic environment for organisms living as free drifters in the water column remains a challenge. Here, we analyzed the transcriptome wide mRNA sequences, as well as the morphology of 18 strains of Alexandrium minutum, a dinoflagellate responsible for Harmful Algal Blooms worldwide, to investigate the functional bases of a divergence event. Analysis of the joint site frequency spectrum (JSFS) pointed toward an ancestral divergence in complete isolations followed by a secondary contact resulting in gene flow between the two diverging groups, but heterogeneous across sites. The sites displaying fixed SNPs were associated with a highly restricted gene flow and a strong...
Tipo: Text Palavras-chave: Dinoflagellates; Harmful algal blooms; Populations Genomics; Pseudocryptic species; Speciation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45857/45529.pdf
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First insight on metabolomics workflows and differences between dinoflagellate: characterization and discrimination of modern and ancient strains and their algal growth ArchiMer
Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible...
Tipo: Text
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00440/55199/56664.pdf
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Genetic diversity of the harmful family Kareniaceae (Gymnodiniales, Dinophyceae) in France, with the description of Karlodinium gentienii sp. nov.: A new potentially toxic dinoflagellate ArchiMer
Nezan, Elisabeth; Siano, Raffaele; Boulben, Sylviane; Six, Christophe; Bilien, Gwenael; Cheze, Karine; Duval, Audrey; Le Panse, Sophie; Quere, Julien; Chomerat, Nicolas.
The family Kareniaceae is mostly known in France for recurrent blooms of Karenia mikimotoi in the Atlantic, English Channel, and Mediterranean Sea and for the unusual green discoloration in the saltwater lagoon of Diana (Corsica) caused by Karlodinium corsicum in April 1994. In terms of diversity, this taxonomic group was long overlooked owing to the difficult identification of these small unarmored dinoflagellates. In this study, thanks to the molecular characterization performed on single cells from field samples and cultures, twelve taxonomic units were assigned to the known genera Karenia, Karlodinium and Takayama, whereas one could not be affiliated to any described genus. The molecular phylogeny inferred from the D1–D2 region of the LSU rDNA showed...
Tipo: Text Palavras-chave: Genetic diversity; Haemolytic; Kareniaceae; Pigments; Single cell; Taxonomy.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00225/33611/32310.pdf
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Historical records from dated sediment cores reveal the multidecadal dynamic of the toxic dinoflagellate Alexandrium minutum in the Bay of Brest (France) ArchiMer
Klouch, Khadidja; Schmidt, Sabine; Andrieux-loyer, Francoise; Le Gac, Mickael; Hervio-heath, Dominique; Qui-minet, Zujaila Nohemi; Quere, Julien; Bigeard, Estelle; Guillou, Laure; Siano, Raffaele.
The multiannual dynamic of the cyst-forming and toxic marine dinoflagellate Alexandrium minutum was studied over a time scale of about 150 years by a paleoecological approach based on ancient DNA (aDNA) quantification and cyst revivification data obtained from two dated sediment cores of the Bay of Brest (Brittany, France). The first genetic traces of the species presence in the study area dated back to 1873 ± 6. Specific aDNA could be quantified by a newly-developed real-time PCR assay in the upper core layers, in which the germination of the species (in up to 17–19 year-old sediments) was also obtained. In both cores studied, our quantitative paleogenetic data showed a statistically significant increasing trend in the abundance of A. minutum ITS1 rDNA...
Tipo: Text Palavras-chave: Paleoecology; Ancient DNA; Real-time PCR; Harmful algal blooms (HAB); Dinoflagellates; Coastal ecology.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00334/44516/44212.pdf
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Human-induced river runoff overlapping natural climate variability over the last 150 years: Palynological evidence (Bay of Brest, NW France) ArchiMer
Lambert, Clement; Penaud, Aurelie; Vidal, Muriel; Klouch, Khadidja; Gregoire, Gwendoline; Ehrhold, Axel; Eynaud, Frederique; Schmidt, Sabine; Ragueneau, Olivier; Siano, Raffaele.
For the first time a very high resolution palynological study (mean resolution of 1 to 5 years) was carried out over the last 150 years in a French estuarine environment (Bay of Brest; NW France), allowing direct comparison between the evolution of landscapes, surface water, and human practices on Bay of Brest watersheds, through continental (especially pollen grains) and marine (phytoplanktonic microalgae: cysts of dinoflagellates or dinocysts) microfossils. Thanks to the small size of the watersheds and the close proximity of the depositional environment to the mainland, the Bay of Brest represents an ideal case study for palynological investigations. Palynological data were then compared to published palaeo-genetic analyses conducted on the same core...
Tipo: Text Palavras-chave: Pollen grains; Dinoflagellate cysts; Estuarine dynamics; River runoff; Climate variability.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00410/52110/52813.pdf
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Innovative metabolomic workflows to study growth kinetics dinoflagellate cysts revived from modern and ancient sediments ArchiMer
Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf
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Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing ArchiMer
Massana, Ramon; Gobet, Angelique; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Chambouvet, Aurelie; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Dolan, John R.; Dunthorn, Micah; Edvardsen, Bente; Forn, Irene; Forster, Dominik; Guillou, Laure; Jaillon, Olivier; Kooistra, Wiebe H. C. F.; Logares, Ramiro; Mahe, Frederic; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Probert, Ian; Romac, Sarah; Richards, Thomas; Santini, Sebastien; Shalchian-tabrizi, Kamran; Siano, Raffaele; Simon, Nathalie; Stoeck, Thorsten; Vaulot, Daniel; Zingone, Adriana; De Vargas, Colomban.
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together,...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00275/38594/37190.pdf
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