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Registros recuperados: 12
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Assessment of the molecular weight distribution of tannin fractions through MALDI-TOF MS analysis of protein-tannin complexes Inra
Mane, C.; Sommerer, N.; Yalcin, T.; Cheynier, V.; Cole, R.B.; Fulcrand, H..
An innovative mass spectrometry method was developed for determining mass distributions of tannin fractions that cannot be approached through direct MALDI-TOF analysis. It was applied to three procyanidin fractions with average degrees of polymerizations = 3, 9, and 28, respectively, and one gallotannin fraction (Tara tannin). The proposed approach consists of MALDI-TOF analysis of the soluble complexes formed between these tannin fractions and bovine serum albumin (BSA). Complexes were detected as an unresolved "hump" following the BSA signal, and spectra were mathematically processed to determine the parameters relative to the protein-tannin complexes, which are the number-average molecular weight (M-n), the weight-average molecular weight (M-w), and the...
Tipo: Journal Article Palavras-chave: POIDS MOLECULAIRE TANNIN; SPECTROMETRY METHOD; PROCYANIDIN FRACTION; WINE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f0f47ea0&uri=/notices/prodinra1/2011/01/
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Combined mass mapping and biochemical characterization of grape beta-glycosidase-enriched extract Inra
Sarry, J.E.; Grimplet, J.; Sommerer, N.; Vallier, M.J.; Pradal, M.; Mondolot, L.; Andary, C.; Gunata, Z.; Romieu, C..
A beta-glucosidase enzyme activity was enriched from skins of ripe grape berry by cell wall fractionation, hydrophobic interaction and cation-exchange chromatographies. This enriched enzyme extract contained several beta-glycosidase activities hydrolyzing a wide range of synthetic and natural monoglycosides and diglycosides, as well as a beta-fructosidase activity. The enzyme extract was further characterized by two-dimensional gel electrophoresis coupled to peptide mass fingerprinting of eight spots using MALDI-TOF mass spectrometry. No beta-glucosidase but a beta-fructosidase associated to the relevant spot at 66 kDa/pI 5.1 was identified. Taken together all results issued from the biochemical characterization, the substrate specificity and the mass...
Tipo: Journal Article Palavras-chave: BETA-GLYCOSIDASE; MASS MAPPING; SUBSTRATE SPECIFICITY; PROTEOME; GRAPE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c84ba3d7&uri=/notices/prodinra1/2009/02/
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Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides Inra
Shenar, N.; Sommerer, N.; Martinez, J.; Enjalbal, C..
We report our contribution to the :systematic investigation of peptide fragmentations performed on high-performance Tof equipment, operating in MS and MS/MS modes, such as ESI-QqTof and MALDI-Tof/Tof instruments that are commonly available today in proteomic laboratories. Whereas the former analyzer's configuration provides low-energy collision-induced dissociations (CID), the latter allows tunable activation methods of the selected parent ion to induce either metastable laser-induced dissociations (LID) or high-energy CID ('gas on spectra LID'). Fragmentation of the monoprotonated ion of 53 peptides (FW 807-2853 g/mol) was undertaken upon low-energy CID on an ESI-QTof mass spectrometer (Waters) as well as high-energy CID and LID conditions on a MALDI...
Tipo: Journal Article Palavras-chave: PEPTIDES; MS/MS; CID; COLLISION INDUCED DISSOCIATION; LID; LASER INDUCED DISSOCIATION; MALDI-TOF/TOF; ESI-QQTOF.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010a9a536c8&uri=/notices/prodinra1/2010/08/
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Different impact of staining procedures using visible stains and fluorescent dyes for large-scale investigation of proteomes by MALDI-TOF mass spectrometry Inra
Chevalier, F.; Centeno, D.; Rofidal, V.; Tauzin, M.; Martin, O.; Sommerer, N.; Rossignol, M..
Replicate 2-D gels were stained with four visible or fluorescent dyes using published procedures, and48 co-detected spots were selected for contrasting values in abundance, Mr and p I. Success rate ofidentification and sequence coverage were affected in a dye-dependent manner by the three parameters.Frequency of missed cleavages and recovery of sulfur-containing peptides also depended on the dye.Finally, the dataset was used to predict the number of proteins identifiable when integrating thedifferential contribution of each parameter. Sypro Ruby appeared to combine several favorablefeatures: no dependence of the identification rate upon the physicochemical properties of proteins,no impact on frequency of missed cleavages, and a higher predicted...
Tipo: Journal Article Palavras-chave: VISIBLE STAINING; FLUORESCENT DYES; PEPTIDE MASS FINGERPRINT; PROTEIN IDENTIFICATION; SEQUENCE COVERAGE; PROTEIN PROPERTIES; MISSED CLEAVAGES.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200855bb4aa4&uri=/notices/prodinra1/2008/04/
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Excreted/secreted proteins from Trypanosome procyclic strains Inra
Nten, C.M.A.; Sommerer, N.; Rofidal, V.; Hirtz, C.; Rossignol, M.; Cuny, G.; Peltier, J.B.; Geiger, A..
Trypanosoma secretome was shown to be involved in parasite virulence and is suspected of interfering in parasite life-cycle steps such as establishment in the Glossina midgut, metacyclogenesis. Therefore, we attempted to identify the proteins secreted by procyclic strains of T. brucei gambiense and T. brucei brucei, responsible for human and animal trypanosomiasis, respectively. Using mass spectrometry, 427 and 483 nonredundant proteins were characterized in T. brucei brucei and T. brucei gambiense secretomes, respectively; 35% and 42% of the corresponding secretome proteins were specifically secreted by T. brucei brucei and T. brucei gambiense, respectively, while 279 proteins were common to both subspecies. The proteins were assigned to 12 functional...
Tipo: Journal Article Palavras-chave: TRYPANOSOME; SECRETOME; TRANSMISSION; MOUCHE TSE-TSE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201013996fd2&uri=/notices/prodinra1/2010/08/
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Identification of S-methylmethionine in Petit Manseng grapes as dimethyl sulphide precursor in wine Inra
Loscos, N.; Ségurel, M.; Dagan, L.; Sommerer, N.; Marlin, T.; Baumes, R..
A procedure for the extraction of free amino acids was applied to isolate S-methylmethionine (SMM) from late harvest Petit Manseng grapes. Grapes were destemmed and crushed, and the obtained clarified must was percolated through cation-exchange resins (Dowex 50 WX4-100). The retained compounds were eluted with ammonia solution and the extract was finally concentrated. Taking into account the potential DMS (PDMS using heat-alkaline treatment assay) of the initial grape juice used (51.5 nmol mL(-1)) and the concentration factor of the extract (17.9-fold), the PDMS of the final extract (678 nmol mL(-1)) gave an overall recovery of 73.5% for juice SMM. This compound was identified and quantified (484.5 nmol mL(-1) relatively to [H-2(3)]-SMM used as internal...
Tipo: Journal Article Palavras-chave: AROMA; GRAPE; WINE; DIMETHYL SULPHIDE (DMS); PRECURSOR; S-METHYLMETHIONINE (SMM); MATRIX-ASSISTED DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF-MS).
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201019d8f2f7&uri=/notices/prodinra1/2010/08/
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Major proteome variations associated with cherry tomato pericarp development and ripening Inra
Faurobert, M.; Mihr, M.; Bertin, N.; Pawlowski, T.; Negroni, L.; Sommerer, N.; Causse, M..
Tipo: Journal Article Palavras-chave: DEVELOPPEMENT DU FRUIT; PERICARPE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200743f25eae&uri=/notices/prodinra1/2009/11/
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Multiple phosphorylations in the C-terminal tail of plant plasma membrane aquaporins : role in sub-cellular trafficking of AtPIP2;1 in response to salt stress Inra
Prak, S.; Hem, S.; Boudet, J.; Viennois, G.; Sommerer, N.; Rossignol, M.; Maurel, C.; Santoni, V..
Aquaporins form a family of water and solute channel proteins and are present in most living organisms. In plants, aquaporins play an important role in the regulation of root water transport in response to abiotic stresses. In this work, we investigated the role of phosphorylation of Plasma membrane Intrinsic Protein (PIP) aquaporins in the Arabidopsis thaliana root by a combination of quantitative mass spectrometry and cellular biology approaches. A novel phosphoproteomic procedure that involves plasma membrane purification, phosphopeptide enrichment with TiO2 columns, and systematic mass spectrometry sequencing revealed multiple and adjacent phosphorylation sites in the C-terminal tail of several AtPIPs. Six of these sites had not been previously...
Tipo: Journal Article Palavras-chave: AQUAPORIN; CHANNEL PROTEINS; WATER TRANSPORT; PLASMA MEMBRANE; SALT STRESS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009724b97eb&uri=/notices/prodinra1/2010/09/
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Novel subsets of the Arabidopsis plasmalemma phosphoproteome identify phosphorylation sites in secondary active transporters Inra
Hem, S.; Rofidal, V.; Sommerer, N.; Rossignol, M..
The generation of novel subsets of phosphorylation sites is needed to complement the present Arabidopsis plasma membrane phosphoproteinrepertoire, where several families of proteins are under-represented. In this work, different combinations of chromatographicsteps were first compared for capacity to resolve model phosphopeptides before characterisation from PSD fragments in MALDI MS/MS. Nearly half of the phosphorylation sites detected in the Arabidopsis plasmalemma using the optimised procedure were novel, andtwo-thirds of protein accessions identified secondary active transporters. These included phosphate/H+ symporters, ammonium andnitrate transporters, different alkali cation exchangers, a urea/H+ symporter, a glucose transporter, a purine permease,...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS; PLASMA MEMBRANE; SOS1; PHOSPHOPEPTIDE; SECONDARY ACTIVE TRANSPORTER; AMT1.1; CHX17; DUR3; PHT1.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200861196512&uri=/notices/prodinra1/2008/05/
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Proteins and proteolysis in pre-term and term human milk and possible implications for infant formulae Inra
Armaforte, E.; Curran, E.; Huppertz, T.; Ryan, C.A.; Caboni, M.F.; O'Connor, P.M.; Ross, R.P.; Hirtz, C.; Sommerer, N.; Chevalier, F.; Kelly, A.L..
Understanding the differences between the protein system of human milk and bovine milk is critical in the development of infant formulae. In this study, the proteins of bovine milk and a bovine-based whey-dominant infant formula were compared with those of human milk for infants born prematurely (pre-term) or at full term (term). The protein distribution of infant formula differed significantly from that of either type of human milk. A proteomic comparison between pre-term and term human milk showed a reduction of levels of β-casein and αs-casein and appearance of additional products, corresponding to low molecular weight hydrolysis products of the caseins, in pre-term milk. Pre-term milk samples also had higher total nitrogen concentration and plasmin...
Tipo: Journal Article Palavras-chave: LAIT INFANTILE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010d3a489e4&uri=/notices/prodinra1/2010/09/
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The effect of environmental salinity on the proteome of the sea bass (Dicentrarchus labrax L.) Inra
Ky, C.L.; de Lorgeril, J.; Hirtz, C.; Sommerer, N.; Rossignol, M.; Bonhomme, F..
The European sea bass, Dicentrarchus labrax L., tolerates a range of salinities from freshwater to hyper-saline. To study differences in protein expression, fish were reared in both freshwater and seawater. After 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. In all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. Fifty differential protein spots were excised from a colloidal Coomassie-stained gel. Nine separate protein spots were identified unambiguously by mass spectrometry and database searching. Among the six proteins over-expressed in gill cells in seawater, five were cytoskeleton proteins and one was the aromatase...
Tipo: Journal Article Palavras-chave: MALDI-TOF MASS SPECTROMETRY; REAL TIME PCR; SALINITY; SEA BASS; TWO-DIMENSIONAL GEL ELECTROPHORESIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008f070b07f&uri=/notices/prodinra1/2008/04/
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The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor Inra
Delom, F.; Szponarski, W.; Sommerer, N.; Boyer, J.C.; Bruneau, J.M.; Rossignol, M.; Gibrat, R..
Calcofluor is an antifungal compound known to induce structural perturbations of the cell wall by interfering with the synthesis of chitin microfibril. Proteins from a stripped plasma membrane fraction were solubilized with the neutral and non-denaturing detergent, the n-dodecyl beta-D-maltoside. Proteins were then resolved using a recently described ion-exchange chromatography (IEC)/lithium dodecyl sulfate (LDS)-PAGE procedure. Nearly 90 proteins were identified and clustered, based on their pI, molecular weight, abundance and/or hydrophobicity. This method was then applied to profile the plasma membrane response to calcofluor. The LDS-PAGE patterns obtained from whole plasma membrane proteins were similar for the non-treated and calcofluor-treated...
Tipo: Journal Article Palavras-chave: PROTEINE MEMBRANAIRE CELL WALL; LIQUID CHROMATOGRAPHY; PLASMA MEMBRANE PROTEINS; SACCHAROMYCES CEREVISIAE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20072157644e&uri=/notices/prodinra1/2007/04/
Registros recuperados: 12
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