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Registros recuperados: 11
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A semi-automated Raman micro-spectroscopy method for morphological and chemical characterizations of microplastic litter ArchiMer
Frere, L.; Paul-pont, I.; Moreau, Julien; Soudant, P.; Lambert, C.; Huvet, Arnaud; Rinnert, Emmanuel.
Every step of microplastic analysis (collection, extraction and characterization) is time-consuming, representing an obstacle to the implementation of large scale monitoring. This study proposes a semi-automated Raman micro-spectroscopy method coupled to static image analysis that allows the screening of a large quantity of microplastic in a time-effective way with minimal machine operator intervention. The method was validated using 103 particles collected at the sea surface spiked with 7 standard plastics: morphological and chemical characterization of particles was performed in < 3 h. The method was then applied to a larger environmental sample (n = 962 particles). The identification rate was 75% and significantly decreased as a function of particle...
Tipo: Text Palavras-chave: Microplastics; Raman micro-spectroscopy; Surface seawater; Morphology; Environmental monitoring; Automating.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00357/46802/46976.pdf
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Bioactive extracellular compounds produced by the dinoflagellate Alexandrium minutum are highly detrimental for oysters ArchiMer
Castrec, Justine; Soudant, P.; Payton, L.; Tran, D.; Miner, Philippe; Lambert, C.; Le Goic, N.; Huvet, Arnaud; Quillien, Virgile; Boullot, F.; Amzil, Zouher; Hegaret, Helene; Fabioux, C..
Blooms of the dinoflagellate Alexandrium spp., known as producers of paralytic shellfish toxins (PSTs), are regularly detected on the French coastline. PSTs accumulate into harvested shellfish species, such as the Pacific oyster Crassostrea gigas, and can cause strong disorders to consumers at high doses. The impacts of Alexandrium minutum on C. gigas have often been attributed to its production of PSTs without testing separately the effects of the bioactive extracellular compounds (BECs) with allelopathic, hemolytic, cytotoxic or ichthyotoxic properties, which can also be produced by these algae. The BECs, still uncharacterized, are excreted within the environment thereby impacting not only phytoplankton, zooplankton but also marine invertebrates and...
Tipo: Text Palavras-chave: Harmful algal bloom (HAB); Crassostrea gigas; Paralytic shellfish toxins (PST); Bioactive extracellular compounds (BEC); Histology; Behavior.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00435/54637/56042.pdf
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Evaluation of the impact of polyethylene microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae ArchiMer
Mazurais, David; Ernande, Bruno; Quazuguel, Patrick; Severe, Armelle; Huelvan, Christine; Madec, Lauriane; Mouchel, Olivier; Soudant, P.; Robbens, J.; Huvet, Arnaud; Zambonino-infante, J..
Microplastics are present in marine habitats worldwide and may be ingested by low trophic organisms such as fish larvae, with uncertain physiological consequences. The present study aims at assessing the impact of polyethylene (PE 10-45µM) microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae. Fish were fed an inert diet including 0, 104 and 105 fluorescent microbeads per gram from 7 until 43 days post-hatching (dph). Microbeads were detected in the gastrointestinal tract in all fish fed diet incorporating PE. Our data revealed an efficient elimination of PE beads from the gut since no fluorescent was observed in the larvae after 48h depuration. While the mortality rate increased significantly with the amount of microbeads scored per...
Tipo: Text Palavras-chave: Microplastic; Fish; Larvae; Ingestion; Survival.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00279/39057/37621.pdf
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Impacts of Deepwater Horizon oil and associated dispersant on early development of the Eastern oyster Crassostrea virginica ArchiMer
Vignier, J.; Donaghy, L.; Soudant, P.; Chu, F. L. E.; Morris, J. M.; Carney, M. W.; Lay, C.; Krasnec, M.; Robert, Rene; Volety, A. K..
The explosion of the Deepwater Horizon (DWH) oil platform resulted in large amounts of crude oil and dispersant Corexit 9500A® released into the Gulf of Mexico and coincided with the spawning season of the oyster, Crassostrea virginica. The effects of exposing gametes and embryos of C. virginica to dispersant alone (Corexit), mechanically (HEWAF) and chemically dispersed (CEWAF) DWH oil were evaluated. Fertilization success and the morphological development, growth, and survival of larvae were assessed. Gamete exposure reduced fertilization (HEWAF: EC201 h = 1650 μg tPAH50 L− 1; CEWAF: EC201 h = 19.4 μg tPAH50 L− 1; Corexit: EC201 h = 6.9 mg L− 1). CEWAF and Corexit showed a similar toxicity on early life stages at equivalent nominal concentrations....
Tipo: Text Palavras-chave: Deepwater Horizon oil; Oyster; Fertilization; CEWAF; Corexit; PAH.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00277/38853/42693.pdf
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Influence of environmental and anthropogenic factors on the composition, concentration and spatial distribution of microplastics: A case study of the Bay of Brest (Brittany, France) ArchiMer
Frere, L.; Paul-pont, I.; Rinnert, Emmanuel; Petton, Sebastien; Jaffre, J; Bihannic, Isabelle; Soudant, P.; Lambert, C.; Huvet, Arnaud.
The concentration and spatial distribution of microplastics in the Bay of Brest (Brittany, France) was investigated in two surveys. Surface water and sediment were sampled at nine locations in areas characterized by contrasting anthropic pressures, riverine influences or water mixing. Microplastics were categorized by their polymer type and size class. Microplastic contamination in surface water and sediment was dominated by polyethylene fragments (PE, 53–67%) followed by polypropylene (PP, 16–30%) and polystyrene (PS, 16–17%) microparticles. The presence of buoyant microplastics (PE, PP and PS) in sediment suggests the existence of physical and/or biological processes leading to vertical transfer of lightweight microplastics in the bay. In sediment (upper...
Tipo: Text Palavras-chave: Microplastic; Coastal ecosystem; Surface water; Sediment; Raman spectroscopy; Hydrodynamic; Dispersal particle modelling.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00379/48994/51083.pdf
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Influence of gametogenesis pattern and sex on paralytic shellfish toxin levels in triploid Pacific oyster Crassostrea gigas exposed to a natural bloom of Alexandrium minutum ArchiMer
Hermabessiere, Ludovic; Fabioux, C.; Lassudrie, Malwenn; Boullot, F.; Long, Marc; Lambert, Christophe; Le Goic, N.; Gouriou, Jeremie; Le Gac, Mickael; Chapelle, Annie; Soudant, P.; Hegaret, Helene.
This study investigated the effect of gametogenesis pattern and sex on levels of paralytic shellfish toxins (PST) accumulated by triploid oysters Crassostrea gigas exposed to a natural bloom of the toxic dinoflagellate Alexandrium minutum in the Bay of Brest (Western Brittany, France), over the summer 2014. Toxin accumulation in oysters was proposed to be influenced by the proportion of energy allocated to reproduction versus other metabolisms, as proposed by Haberkorn et al. (2010). Thus, we hypothesized that triploid oysters with different gametogenesis patterns (α or β, producing respectively numerous gametes or rare gametes) could result in differences in toxin accumulation. Toxin level could also be different according to the gender of the oysters. To...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium minutum; Harmful algal bloom (HAB); Paralytic shellfish toxin (PST); Gametogenesis pattern; Triploid.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00307/41801/41010.pdf
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Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition ArchiMer
Da Costa, F.; Petton, Bruno; Mingant, Christian; Bougaran, Gael; Rouxel, Catherine; Quere, Claudie; Wikfors, G. H.; Soudant, P.; Robert, Rene.
Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate...
Tipo: Text Palavras-chave: Larvae; Lipids; Oyster; Tisochrysis lutea.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00251/36258/36063.pdf
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Lethal and sub-lethal effects of Deepwater Horizon slick oil and dispersant on oyster (Crassostrea virginica) larvae ArchiMer
Vignier, J.; Soudant, P.; Chu, F. L. E.; Morris, J. M.; Carney, M. W.; Lay, C. R.; Krasnec, M. O.; Robert, Rene; Volety, A. K..
In April 2010, crude oil was spilled from the Deepwater Horizon (DWH) oil platform for 87 days, coincident with the spawning season and recruitment of the oyster, Crassostrea virginica, in the Gulf of Mexico. Impacts of acute exposures to surface-collected DWH oil (HEWAF), dispersed oil (CEWAF) and dispersant alone (Corexit 9500A®) on planktonic larval stages of C. virginica (veliger, umbo and pediveliger) were tested in the laboratory. Exposures to HEWAF, CEWAF and dispersant were toxic to larvae impairing growth, settlement success and ultimately survival. Larval growth and settlement were reduced at concentrations of tPAH50 ranging from 1.7 to 106 μg L−1 for HEWAF and 1.1–35 μg L−1 for CEWAF, concentrations well within the range of water sampled during...
Tipo: Text Palavras-chave: Deepwater Horizon oil spill; Crassostrea virginica; Corexit 9500A (R); Larvae; Growth; Settlement; PAH.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00345/45572/45181.pdf
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REPAMEP - Réponse des palourdes aux stress environnementaux combinant métaux, efflorescences toxiques et pathogènes - Programme LITEAU. ArchiMer
Baudrimont, M.; Gonzalez, P.; Hegaret, H.; Khayati, Alice; Lassus, Patrick; Legeay, A.; De Montaudouin, Xavier; Raymond, N.; Soudant, P.; Tran, D..
The originality of this project consists in the interdisciplinary approach gathering the impact of toxic phytoplankton blooms and metallic pollution on health (behaviour, stress, immunity, genetic) and disease development of the Manila clam, an economieally important species. The Manila clam Ruditapes philippinarum is the second exploited bivalve in the world. It was introduced in Arcachon bay during the 80's and is today exploited by professional fishermen (57 licences). A 50% decrease of the stock was observed during the last five years. Beyond fishing pressure, a PhD thesis identified several diseases as potential threats, like perkinsosis (=Dermo) and brown muscle disease (BMD). The project confirms the settlement of these pathologies with high...
Tipo: Text Palavras-chave: Palourde japonaise; Pathologie; Efflorescences toxiques; Interactions microbiennes; Écotoxicologie; Multistress; Manila clam; Pathology; Toxic blooms; Microbial interactions; Ecotoxicology; Multistress.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00156/26745/24825.pdf
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Sensitivity of eastern oyster ( Crassostrea virginica) spermatozoa and oocytes to dispersed oil: Cellular responses and impacts on fertilization and embryogenesis ArchiMer
Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P..
The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were...
Tipo: Text Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf
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Why is Asari (= Manila) clam Ruditapes philippinarum fitness poor in Arcachon Bay: a meta-analysis to answer? ArchiMer
De Montaudouin, X.; Lucia, M.; Binias, C.; Lassudrie, Malwenn; Baudrimont, M.; Legeay, A.; Raymond, N.; Jude-lemeilleur, F.; Lambert, C.; Le Goic, N.; Garabetian, F.; Gonzalez, P.; Hegaret, Helene; Lassus, Patrick; Mehdioub, W.; Bourasseau, L.; Daffe, G.; Paul-pont, I.; Plus, Martin; Do, V. T.; Meisterhans, G.; Mesmer-dudons, N.; Caill-milly, Nathalie; Sanchez, Florence; Soudant, P..
Asari (= Manila) clam, Ruditapes philippinarum, is the second bivalve mollusc in terms of production in the world and, in many coastal areas, can beget important socio-economic issues. In Europe, this species was introduced after 1973. In Arcachon Bay, after a decade of aquaculture attempt, Asari clam rapidly constituted neo-naturalized population which is now fished. However, recent studies emphasized the decline of population and individual performances. In the framework of a national project (REPAMEP), some elements of fitness, stressors and responses in Arcachon bay were measured and compared to international data (41 publications, 9 countries). The condition index (CI=flesh weight/shell weight) was the lowest among all compared sites. Variation in...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Condition index; Perkinsus; Brown Muscle Disease; Element contamination; Immune system; Arcachon Bay.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00280/39123/37676.pdf
Registros recuperados: 11
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