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Registros recuperados: 13
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A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery BABT
Severo Jr.,João Batista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.
Tipo: Info:eu-repo/semantics/article Palavras-chave: BSA; Protein adsorption; Breakthrough curves; Amberlite IRA 410 ion-exchange resin; Distribution residence time study; Richardson-Zack coefficient.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200021
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Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp BABT
Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024
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Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation BABT
Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002
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Imobilização de enzimas de milho maltado em gel Ciênc. Tecnol. Aliment.
Silva,Rosa Luísa de Farias Oliveira Bezerra e; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
Este trabalho objetivou a otimização da imobilização das enzimas amilases extraídas do malte do milho, usando alginato de sódio. A concentração do malte no extrato, a porcentagem de alginato de sódio e o pH foram usados como fatores que influenciam na imobilização das enzimas. Os resultados mostraram que as melhores condições de imobilização foram obtidas quando se usou as soluções de malte de milho em duas faixas de concentrações, uma entre 3,75 a 5 g.L-1 e outra entre 15 a 16,25 g.L-1, em pH entre 4,83 a 6,6 e 4% (m/v) de alginato de sódio, condições nas quais se conseguiu imobilizar 100% das enzimas com baixa perda de atividade. Este trabalho mostrou como se obter amilases de malte de milho imobilizadas por oclusão em alginato de sódio e que podem ser...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Imobilização; Amilases; Alginato de sódio; Malte de milho; Oclusão; Biocatalisador.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000300021
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Microalgae: Cultivation Aspects and Bioactive Compounds BABT
Coêlho,Diego de Freitas; Tundisi,Louise Lacalendola; Cerqueira,Keilla Santos; Rodrigues,Jacqueline Rego da Silva; Mazzola,Priscila Gava; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
Abstract Microalgae are aquatic unicellular microorganisms that can be found both in freshwater and marine systems; are capable of photosynthesis; and can grow as individual cells or associated in chains or small colonies. Microalgae cultivation has gained large momentum among researchers in the past decades due to their ability to produce value metabolites, remarkable photosynthetic efficiency, and versatile nature. The wide technological potential, and thus increasing amount of scattered knowledge, may become the very first barrier that a post graduating student, or any non-specialist reader, will face when introduced to the subject. In this review paper, we access the core aspects of microalgae technology, covering their main characteristics, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Microalgae Selection and Isolation; Green Products; Microalgae Cultivation; Bioactive Compounds.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000101000
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Optimization of drying process of Zea Mays malt to use as alternative source of amylolytics enzymes BABT
Biazus,Joana Paula Menezes; Souza,Anderson Gomes; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambougi,Elias Basile.
This work aimed to study the drying process optimization of maize (Zea Mays) malt for obtaining maize malt, without affecting enzymatic activity of alpha e beta-amylases from maize malt. Results showed that dryer operation must occur in zone at 54°C and 5.18-6 h process time. The maize malt obtained had good enzymatic properties.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha and beta -amylases enzymes; Maize (Zea mays) malt/drying process; Optimization.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400023
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Otimização da secagem do malte de Zea mays Ciênc. Tecnol. Aliment.
Biazus,Joana Paula Menezes; Souza,Roberto Rodrigues de; Curvelo-Santana,José Carlos; Tambourgi,Elias Basile.
Neste trabalho foi feita a otimização do processo de secagem do malte de milho (Zea mays) com o objetivo de gerar um derivado de alto valor comercial sem reduzir a atividade das enzimas presentes neste, alfa e beta - amilases, e tentar agregar valor à cultura do milho. Modelos de curvas de secagem foram usados para simular as isotermas de secagem. Um planejamento fatorial completo do tipo 2² foi feito, usando como fatores o tempo (5,18; 6; 8; 10 e 10,8 h) em torno do tempo de secagem para determinação de umidade em alimento e a temperatura (54, 65 e 76 °C) próxima do ótimo das enzimas e a atividade enzimática no malte como resposta. O modelo que mais se ajustou aos dados de cinética de secagem foi o de Henderson e Henderson, e a umidade média retirada do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzimas alfa e beta-amilases; Malte de milho (Zea mays); Secagem; Otimização.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000400012
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Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2 BABT
Ferreira,Graziela Batista; Evangelista,Alex Ferreira; Severo Junior,João Baptista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile; Jordão,Elizabete.
This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partitioning; Optimization; Aqueous two-phase systems; Maize malt; PEG 6000; CaCl2.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300020
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Production and characterization of amylases from Zea mays malt BABT
Biazus,Joana Paula Menezes; Souza,Roberto Rodrigues de; Márquez,Jesus Espinoza; Franco,Telma Teixeira; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In this work the α and β-amylase enzymes were obtained from maize (Zea mays) malt and were biochemistry characterized. A germination study to obtain the maize malt with good amylase activity was made. The maize seeds were germinated in laboratory and the enzymatic activity was measured daily. Activity dependence to germination time were fitted to an exponential model (A=A0eµt), which showed that the behaviour of enzymatic activity in the germination process was similar to the growth of the microorganism. Its model could be applied to describe the mechanism of α-amylase production for each maize varieties and others cereals. Maize malt characterization showed that α and β-amylase had optimal pH between 4-6.5, optimal temperature 50 and 90ºC, and molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize seed; Malting; Α and β-amylase; Biochemistry characterization; Germination model.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400024
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Purificação de amilases de malte de Zea mays Ciênc. Tecnol. Aliment.
Biazus,Joana Paula Menezes; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambourgi,Elias Basile.
Neste trabalho realizou-se a purificação das enzimas α e β-amilases de malte de milho combinando os processos de adsorção em leito expandido com o fracionamento por etanol. Alimentaram-se 5 mL de uma solução a 2% de malte de milho em tampão fosfato 0,05 M a pH 5 em uma coluna contendo as resinas de troca iônica Amberlite IRA 410, em leito fixo de 4 cm fluidizado para 8 cm. A eluição foi promovida com NaCl 0,25M com 14 mL/minuto de vazão descendente e o material eluído foi fracionado com álcool etílico nas concentrações de 40-70%, 50-80% e 60-90% (v/v) a 20 e 30 ºC. A recuperação da atividade e o fator de purificação foram de 95% e 23 vezes, respectivamente, na primeira etapa. A melhor condição de fracionamento das enzimas foi apresentada a 50-80% de álcool...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adsorção em leito expandido; Precipitação; Amilases; Malte de milho; Álcool etílico; Resina de troca iônica.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100032
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Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane BABT
Severo Júnior,João Baptista; Oliveira,Laura Sampaio de Sá; Sardeiro,Fernanda Silva; Souza,Roberto Rodrigues de; Lopes,Francisco Luiz Gumes; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
This work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The response surface analysis showed that the best operation condition for amylolitics enzymes recovery by hollow fiber membrane was 0.05 bar and pH 5.00, while the enzymes were purified about of 26 times.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hollow fiber membrane; Corn malt; Alpha and beta amylase; RSM.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017
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Use of simulated annealing in standardization and optimization of the acerola wine production Ciênc. Tecnol. Aliment.
Almeida,Sheyla dos Santos; Alves,Wonder Alexandre Luz; Araújo,Sidnei Alves de; Santana,José Carlos Curvelo; Narain,Narendra; Souza,Roberto Rodrigues de.
In this study, seven wine samples were prepared varying the amount of pulp of acerola fruits and the sugar content using the simulated annealing technique to obtain the optimal sensory qualities and cost for the wine produced. S. cerevisiae yeast was used in the fermentation process and the sensory attributes were evaluated using a hedonic scale. Acerola wines were classified as sweet, with 11°GL of alcohol concentration and with aroma, taste, and color characteristics of the acerola fruit. The simulated annealing experiments showed that the best conditions were found at mass ratio between 1/7.5-1/6 and total soluble solids between 28.6-29.0 °Brix, from which the sensory acceptance scores of 6.9, 6.8, and 8.8 were obtained for color, aroma, and flavor,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Simulated annealing; Sensory quality; Acerola; Wine; Optimization; Production cost.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200011
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Viability in the production of a drug extracted from Ananas comosus by a flat membrane system BABT
Lopes,Francisco Luiz Gumes; Sbruzzi,Dalva; Barros,Kleber Vanio Gomes; Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambourgi,Elias Basile.
The aim of this work was to study the production of e bromelain from the Ananas comosus L. Merril, by determining the process conditions using flat membranes. The production system modeling generated a hyperbolical curve and the optimization by response surfaces showed an influence of the transmembrane pressure higher than the pH influence. The cost of the production of bromelain from A. comosus was estimated 9 to 13 times lower than Sigma's retail sales price and 6.5 to 8.5 times lower than when this enzyme was obtained through a liquid-liquid extraction, which showed the economical feasibility of the process.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus; Bromelain; Flat membrane; Production cost; Optimization.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300018
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