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Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus Scientia Agricola
Spadotti,David Marques de Almeida; Wassano,Débora Targino; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo Aranha; Inoue-Nagata,Alice Kazuko.
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Potyvirus; Nucleotide sequence; Genetic diversity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000200187
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Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR BABT
Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate.
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454
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Fungicide application can improve production of tomato coinfected with Begomovirus and Crinivirus PAB
Guimarães,Leysimar Ribeiro Pitzr; Nozaki,Denise Nakada; Moura,Mônika Fecury; Spadotti,David Marques de Almeida; Mituti,Tatiana; Krause-Sakate,Renate; Pavan,Marcelo Agenor.
Abstract: The objective of this work was to evaluate the effect of fungicide application on the concentration of Tomato severe rugose virus (ToSRV, Begomovirus) in the 'Mariana' hybrid tomato coinfected with ToSRV and Tomato chlorosis virus (ToCV, Crinivirus) and the progression of viral concentration by qPCR, as well as to quantify fruit yield and quality. Experiment I consisted in the application of fungicides after sowing (pretreatment): pyraclostrobin+metiram (P+M) (at 3 g L-1) + boscalid (B) (at 0.3 g L-1), followed by biweekly sprayings with P+M (4 g L-1); in experiment II, there was no application at sowing (control treatment), only 4 g L-1 P+M biweekly. ToSRV and ToCV transmissions were performed using sweetpotato whiteflies (Bemisia tabaci,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum lycopersicum; Boscalid; Metiram; Pyraclostrobin; ToCV; ToSRV..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000600435
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