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Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis Electron. J. Biotechnol.
Federici,María Teresa; Marcondes,Jackson A; Picchi,Simone Cristina; Stuchi,Eduardo S; Fadel,André Luiz; Laia,Marcelo L; Lemos,Manoel Victor F; Lemos,Eliana Gertrudes Macedo.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding...
Tipo: Journal article Palavras-chave: Citrus; Microarray; Xylella fastidiosa.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300004
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