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| Triques, K.; Piednoir, E.; Dalmais, M.; Schmidt, J.; Le Signor, C.; Sharkey; Caboche, M.; Sturbois, B.. |
| Background: Most enzymatic mutation detection methods are based on the cleavage of heteroduplex DNA by a mismatch-specific endonuclease at mismatch sites and the analysis of the digestion product on a DNA sequencer. Important limitations of these methods are the availability of a mismatch-specific endonuclease, their sensitivity in detecting one allele in pool of DNA, the cost of the analysis and the ease by which the technique could be implemented in a standard molecular biology laboratory.Results: The co-agroinfiltration of ENDO1 and p19 constructs into N. benthamiana leaves allowed high level of transient expression of a mismatch-specific and sensitive endonuclease, ENDO1 from Arabidopsis thaliana. We demonstrate the broad range of uses of the produced... |
| Tipo: Journal Article |
Palavras-chave: ENDONUCLEASE. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200831e52dbf&uri=/notices/prodinra1/2009/11/ |
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