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Registros recuperados: 10
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A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses Inra
Urbino, C.; Thébaud, G.; Granier, M.; Blanc, S.; Peterschmitt, M..
Viruses of the genus Begomovirus (Geminiviridae) are emerging economically importantplant viruses with a circular, single-stranded DNA genome. Previous studies have shown thatgeminiviruses and RNA viruses exhibit similar mutation frequencies, although geminivirusesare replicated by host DNA polymerases and RNA viruses by their own virus-encoded error-prone RNA-dependent RNA-polymerase. However, the phenotypic effects of naturallyoccurring mutations have never been extensively investigated in geminiviruses, particularlybecause, to be infectious, cloned viral genomes usually require sub-cloning as complete orpartial tandem repeats into a binary vector from Agrobacterium tumefaciens.Using Tomato yellow leaf curl virus (TYLCV), we show here that infectivity...
Tipo: Conference Paper Palavras-chave: GEMINIVIRUS; TOMATO YELLOW LEAF CURL VIRUS; TYLCV.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b88c5e4&uri=/notices/prodinra1/2010/05/
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Accounting for biological variability and sampling scale: a multi-scale approach to building epidemic models Inra
Soubeyrand, S.; Thébaud, G.; Chadoeuf, J..
When one considers the fine-scale spread of an epidemic, one usually knows the sources of biological variability and their qualitative effect on the epidemic process. The force of infection on a susceptible unit depends on the locations and the strengths of the infectious units, and on the environmental and intrinsic factors affecting infectivity and/or susceptibility. The infection probability for the susceptible unit can then be modelled as a function of these factors. Thus, one can build a conceptual model at the fine scale. However, the epidemic is generally observed at a larger scale and one has to build a model adapted to this larger scale. But how can the sources of variation identified at the fine scale be integrated into the model at the larger...
Tipo: Journal Article Palavras-chave: DISPERSION DU POLLEN; DISPERSION STOCHASTIQUE MIXED MODELS; DISEASE SPREAD; INFERENCE; PATTERN; MULTI-SCALE MODELLING; PROPAGULE DISPERSAL; STOCHASTIC DISPERSAL; DISEASE TRANSMISSION; POLLEN DISPERSAL.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200754ac47a0&uri=/notices/prodinra1/2010/11/
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Beyond the consensus: dissecting within-host viral population diversity of Foot-and-Mouth disease virus by using next-generation genome sequencing Inra
Wright, C.F.; Morelli, M.J.; Thébaud, G.; Knowles, N.J.; Herzyk, P.; Paton, D.J.; Haydon, D.T.; King, D.P..
The diverse sequences of viral populations within individual hosts are the starting material for selection and subsequent evolution of RNA viruses such as foot-and-mouth disease virus (FMDV). Using next-generation sequencing (NGS) performed on a Genome Analyzer platform (Illumina), this study compared the viral populations within two bovine epithelial samples (foot lesions) from a single animal with the inoculum used to initiate experimental infection. Genomic sequences were determined in duplicate sequencing runs, and the consensus sequence of the inoculum determined by NGS was identical to that previously determined using the Sanger method. However, NGS revealed the fine polymorphic substructure of the viral population, from nucleotide variants present...
Tipo: Journal Article Palavras-chave: SEQUENCAGE DE NOUVELLE GENERATION; FIEVRE APHTEUSE; PICORNAVIRIDAE.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011b264678b&uri=/notices/prodinra1/2011/04/
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Comment combiner des données génétiques et épidémiologiques pour comprendre la dissémination d'un virus ? Inra
Thébaud, G..
L’identification des événements de transmission successifs par lesquels un agent pathogène sepropage peut être un objectif en soi ou un moyen de paramétrer précisément un modèleépidémiologique. Des informations génétiques (proximité phylogénétique entre isolats) ouépidémiologiques (dates probables d’infection, période infectieuse) peuvent être utilisées pourtendre vers cet objectif. En général, aucune de ces sources d’information n’est suffisante pourreconstituer l’historique des événements de transmission impliqués dans une épidémie ; enrevanche, ces informations de nature différente devraient avoir un effet synergique si l’onparvient à les intégrer dans un cadre probabiliste commun. Du fait de leur taux de mutation trèsélevé, les virus à ARN sont un...
Tipo: Conference Paper Palavras-chave: ARBRE DE TRANSMISSION; DISSEMINATION.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200974cbd63c&uri=/notices/prodinra1/2010/05/
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Distribution of the phenotypic effects of random homologous recombination between two virus species Inra
Vuillaume, F.; Thébaud, G.; Urbino, C.; Forfert, N.; Granier, M.; Froissart, R.; Blanc, S.; Peterschmitt, M..
Recombination has an evident impact on virus evolution and emergence of new pathotypes, and has generated an immense literature. However, the distribution of phenotypic effects caused by genome-wide random homologous recombination has never been formally investigated. Previous data on the subject have promoted the implicit view that most viral recombinant genomes are likely to be deleterious or lethal if the nucleotide identity of parental sequences is below 90%. We decided to challenge this view by creating a bank of near-random recombinants between two viral species of the genus Begomovirus (Family Geminiviridae) exhibiting 82% nucleotide identity, and by testing infectivity and in planta accumulation of recombinant clones randomly extracted from this...
Tipo: Journal Article Palavras-chave: BEGOMOVIRUS.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011e65510b1&uri=/notices/prodinra1/2011/05/
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Dynamics of the multiplicity of cellular infection in a plant virus Inra
Gutiérrez, S.; Yvon, M.; Thébaud, G.; Monsion, B.; Michalakis, Y.; Blanc, S..
Recombination, complementation and competition profoundly influence virus evolution and epidemiology. Since viruses are intracellular parasites, the basic parameter determining the potential for such interactions is the multiplicity of cellular infection (cellular MOI), i.e. the number of viral genome units that effectively infect a cell. The cellular MOI values that prevail in host organisms have rarely been investigated, and whether they remain constant or change widely during host invasion is totally unknown. Here, we fill this experimental gap by presenting the first detailed analysis of the dynamics of the cellular MOI during colonization of a host plant by a virus. Our results reveal ample variations between different leaf levels during the course of...
Tipo: Journal Article Palavras-chave: CAULIFLOWER MOSAIC VIRUS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c4b911fc&uri=/notices/prodinra1/2010/12/
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Etude intégrée du développement d'une maladie ré-émergente transmise par vecteur Inra
Thébaud, G.; Chadoeuf, J.; Labonne, G..
Tipo: Conference Proceedings Palavras-chave: MALADIE EMERGENTE; PREVENTION DES RISQUES; VECTEUR DE MALADIE; ESFY; PHYTOPLASME; MALADIE A PHYTOPLASME; INSECTE VECTEUR; MODELE STATISTIQUE; ANALYSE STATISTIQUE; CYCLE BIOLOGIQUE; PCR; REPARTITION SPATIOTEMPORELLE EUROPEAN STONE FRUIT YELLOWS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007abdb29f4&uri=/notices/prodinra1/2007/12/
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Etude intégrée du développement d'une maladie réémergente transmise par vecteur Inra
Thébaud, G.; Chadoeuf, J.; Labonne, G..
Tipo: Book Chapter Palavras-chave: REEMERGENCE; DEPERISSEMENT; ENROULEMENT CHLOROTIQUE DE L'ABRICOTIER.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011e2cd8886&uri=/notices/prodinra1/2011/02/
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Natural multiplicity of infection of plant cells by genomes of Cauliflower Inra
Gutierrez, S.; Yvon, M.; Monsion, B.; Thébaud, G.; Michalakis, Y.; Blanc, S..
The number of viral genomes co-infecting host cells during the course of a systemic infection(the multiplicity of cellular infection, MOI) is a parameter of foremost importance for severalaspects of the biology of viruses. While a low MOI may reduce the intra-cellular competitionbetween genetic variants, it also greatly limits the extent of possible complementation, and de facto reduces the recombination frequency. In contrast, a high MOI would augment thepossibility of trans-complementation between variants, and the recombination frequency, butpotentially also enhance accumulation of defective genomes and increase competition forreplication. These simple examples illustrate the predicted contrasted effects of MOI on viralgenomes and populations, which...
Tipo: Conference Paper Palavras-chave: CAULIFLOWER MOSAIC VIRUS; MULTIPLICITE D'INFECTION .
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c5ae694a&uri=/notices/prodinra1/2010/05/
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Testing the boolean hypothesis in the non-convex case when a bounded grain can be assumed Inra
Chadoeuf, J.; Bacro, J.N.; Thébaud, G.; Labonne, G..
Spatial independence of objects is a strong hypothesis when using boolean models. Methods to test it have then been developed, but only when the objects are convex.We propose here to replace this assumption by a bound assumption of the objects which can be more easily assumed when modeling spatial patterns in ecology and agricultural science. A test is then proposed, based on the length of the voids of the intersection between transect lines and a dilation of the original process related to the bound value. Its application is shown to several examples, together with its extension to an epidemiological case on orchards, where this problem comes from
Tipo: Journal Article Palavras-chave: MODELE BOOLEEN; OBJET CONVEXE; AIRE AREA; BOOLEAN MODEL; SPATIAL PATTERN.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200862d8c540&uri=/notices/prodinra1/2008/04/
Registros recuperados: 10
Primeira ... 1 ... Última
 

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