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| Registros recuperados: 10 | |
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| Urbino, C.; Thébaud, G.; Granier, M.; Blanc, S.; Peterschmitt, M.. |
| Viruses of the genus Begomovirus (Geminiviridae) are emerging economically importantplant viruses with a circular, single-stranded DNA genome. Previous studies have shown thatgeminiviruses and RNA viruses exhibit similar mutation frequencies, although geminivirusesare replicated by host DNA polymerases and RNA viruses by their own virus-encoded error-prone RNA-dependent RNA-polymerase. However, the phenotypic effects of naturallyoccurring mutations have never been extensively investigated in geminiviruses, particularlybecause, to be infectious, cloned viral genomes usually require sub-cloning as complete orpartial tandem repeats into a binary vector from Agrobacterium tumefaciens.Using Tomato yellow leaf curl virus (TYLCV), we show here that infectivity... |
| Tipo: Conference Paper |
Palavras-chave: GEMINIVIRUS; TOMATO YELLOW LEAF CURL VIRUS; TYLCV. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b88c5e4&uri=/notices/prodinra1/2010/05/ |
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| Wright, C.F.; Morelli, M.J.; Thébaud, G.; Knowles, N.J.; Herzyk, P.; Paton, D.J.; Haydon, D.T.; King, D.P.. |
| The diverse sequences of viral populations within individual hosts are the starting material for selection and subsequent evolution of RNA viruses such as foot-and-mouth disease virus (FMDV). Using next-generation sequencing (NGS) performed on a Genome Analyzer platform (Illumina), this study compared the viral populations within two bovine epithelial samples (foot lesions) from a single animal with the inoculum used to initiate experimental infection. Genomic sequences were determined in duplicate sequencing runs, and the consensus sequence of the inoculum determined by NGS was identical to that previously determined using the Sanger method. However, NGS revealed the fine polymorphic substructure of the viral population, from nucleotide variants present... |
| Tipo: Journal Article |
Palavras-chave: SEQUENCAGE DE NOUVELLE GENERATION; FIEVRE APHTEUSE; PICORNAVIRIDAE. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011b264678b&uri=/notices/prodinra1/2011/04/ |
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| Thébaud, G.. |
| L’identification des événements de transmission successifs par lesquels un agent pathogène sepropage peut être un objectif en soi ou un moyen de paramétrer précisément un modèleépidémiologique. Des informations génétiques (proximité phylogénétique entre isolats) ouépidémiologiques (dates probables d’infection, période infectieuse) peuvent être utilisées pourtendre vers cet objectif. En général, aucune de ces sources d’information n’est suffisante pourreconstituer l’historique des événements de transmission impliqués dans une épidémie ; enrevanche, ces informations de nature différente devraient avoir un effet synergique si l’onparvient à les intégrer dans un cadre probabiliste commun. Du fait de leur taux de mutation trèsélevé, les virus à ARN sont un... |
| Tipo: Conference Paper |
Palavras-chave: ARBRE DE TRANSMISSION; DISSEMINATION. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200974cbd63c&uri=/notices/prodinra1/2010/05/ |
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| Vuillaume, F.; Thébaud, G.; Urbino, C.; Forfert, N.; Granier, M.; Froissart, R.; Blanc, S.; Peterschmitt, M.. |
| Recombination has an evident impact on virus evolution and emergence of new pathotypes, and has generated an immense literature. However, the distribution of phenotypic effects caused by genome-wide random homologous recombination has never been formally investigated. Previous data on the subject have promoted the implicit view that most viral recombinant genomes are likely to be deleterious or lethal if the nucleotide identity of parental sequences is below 90%. We decided to challenge this view by creating a bank of near-random recombinants between two viral species of the genus Begomovirus (Family Geminiviridae) exhibiting 82% nucleotide identity, and by testing infectivity and in planta accumulation of recombinant clones randomly extracted from this... |
| Tipo: Journal Article |
Palavras-chave: BEGOMOVIRUS. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011e65510b1&uri=/notices/prodinra1/2011/05/ |
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| Gutiérrez, S.; Yvon, M.; Thébaud, G.; Monsion, B.; Michalakis, Y.; Blanc, S.. |
| Recombination, complementation and competition profoundly influence virus evolution and epidemiology. Since viruses are intracellular parasites, the basic parameter determining the potential for such interactions is the multiplicity of cellular infection (cellular MOI), i.e. the number of viral genome units that effectively infect a cell. The cellular MOI values that prevail in host organisms have rarely been investigated, and whether they remain constant or change widely during host invasion is totally unknown. Here, we fill this experimental gap by presenting the first detailed analysis of the dynamics of the cellular MOI during colonization of a host plant by a virus. Our results reveal ample variations between different leaf levels during the course of... |
| Tipo: Journal Article |
Palavras-chave: CAULIFLOWER MOSAIC VIRUS. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c4b911fc&uri=/notices/prodinra1/2010/12/ |
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| Gutierrez, S.; Yvon, M.; Monsion, B.; Thébaud, G.; Michalakis, Y.; Blanc, S.. |
| The number of viral genomes co-infecting host cells during the course of a systemic infection(the multiplicity of cellular infection, MOI) is a parameter of foremost importance for severalaspects of the biology of viruses. While a low MOI may reduce the intra-cellular competitionbetween genetic variants, it also greatly limits the extent of possible complementation, and de facto reduces the recombination frequency. In contrast, a high MOI would augment thepossibility of trans-complementation between variants, and the recombination frequency, butpotentially also enhance accumulation of defective genomes and increase competition forreplication. These simple examples illustrate the predicted contrasted effects of MOI on viralgenomes and populations, which... |
| Tipo: Conference Paper |
Palavras-chave: CAULIFLOWER MOSAIC VIRUS; MULTIPLICITE D'INFECTION . |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c5ae694a&uri=/notices/prodinra1/2010/05/ |
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| Registros recuperados: 10 | |
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