Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 9
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Analysis of larval oyster grazing by flow cytometry ArchiMer
Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene.
The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice...
Tipo: Text Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf
Imagem não selecionada

Imprime registro no formato completo
Benthic protists: the under-charted majority ArchiMer
Forster, Dominik; Dunthorn, Micah; Mahe, Frederic; Dolan, John R.; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Edvardsen, Bente; Egge, Elianne; Eikrem, Wenche; Gobet, Angelique; Kooistra, Wiebe H. C. F.; Logares, Ramiro; Massana, Ramon; Montresor, Marina; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Romac, Sarah; Shalchian-tabrizi, Kamran; Simon, Nathalie; Richards, Thomas A.; Santini, Sebastien; Sarno, Diana; Siano, Raffaele; Vaulot, Daniel; Wincker, Patrick; Zingone, Adriana; De Vargas, Colomban; Stoeck, Thorsten.
Marine protist diversity inventories have largely focused on planktonic environments, while benthic protists have received relatively little attention. We therefore hypothesize that current diversity surveys have only skimmed the surface of protist diversity in marine sediments, which may harbour greater diversity than planktonic environments. We tested this by analyzing sequences of the hypervariable V4 18S rRNA from benthic and planktonic protist communities sampled in European coastal regions. Despite a similar number of OTUs in both realms, richness estimations indicated that we recovered at least 70% of the diversity in planktonic protist communities, but only 33% in benthic communities. There was also little overlap of OTUs between planktonic and...
Tipo: Text Palavras-chave: Benthic microbial communities; Coastal environments; Protist diversity; High-throughput sequencing.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00341/45233/44658.pdf
Imagem não selecionada

Imprime registro no formato completo
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer ArchiMer
Balzano, S.; Gourvil, P.; Siano, Raffaele; Chanoine, M.; Marie, D.; Lessard, S.; Sarno, D.; Vaulot, Daniel.
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA...
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00114/22491/20172.pdf
Imagem não selecionada

Imprime registro no formato completo
Green Edge ice camp campaigns: understanding the processes controlling the under-ice Arctic phytoplankton spring bloom ArchiMer
Massicotte, Philippe; Amiraux, Rémi; Amyot, Marie-pier; Archambault, Philippe; Ardyna, Mathieu; Arnaud, Laurent; Artigue, Lise; Aubry, Cyril; Ayotte, Pierre; Bécu, Guislain; Bélanger, Simon; Benner, Ronald; Bittig, Henry C; Bricaud, Annick; Brossier, Eric; Bruyant, Flavienne; Chauvaud, Laurent; Christiansen-stowe, Debra; Claustre, Hervé; Cornet-barthaux, Véronique; Coupel, Pierre; Cox, Christine; Delaforge, Aurélie; Dezutter, Thibault; Dimier, Céline; Dominé, Florent; Dufour, Francis; Dufresne, Christiane; Dumont, Dany; Ehn, Jens; Else, Brent; Ferland, Joannie; Forget, Marie-hélène; Fortier, Louis; Gali, Marti; Galindo, Virginie; Gallinari, Morgane; Garcia, Nicole; Gerikas-ribeiro, Catherine; Gourdal, Margaux; Gourvil, Priscilla; Goyens, Clemence; Grondin, Pierre-luc; Guillot, Pascal; Guilmette, Caroline; Houssais, Marie-noëlle; Joux, Fabien; Lacour, Léo; Lacour, Thomas; Lafond, Augustin; Lagunas, José; Lalande, Catherine; Laliberté, Julien; Lambert-girard, Simon; Larivière, Jade; Lavaud, Johann; Lebaron, Anita; Leblanc, Karine; Le Gall, Florence; Legras, Justine; Lemire, Mélanie; Levasseur, Maurice; Leymarie, Edouard; Leynaert, Aude; Lopes Dos Santos, Adriana; Lourenço, Antonio; Mah, David; Marec, Claudie; Marie, Dominique; Martin, Nicolas; Marty, Constance; Marty, Sabine; Massé, Guillaume; Matsuoka, Atsushi; Matthes, Lisa; Moriceau, Brivaela; Muller, Pierre-emmanuel; Mundy, Christopher-john; Neukermans, Griet; Oziel, Laurent; Panagiotopoulos, Christos; Pangazi, Jean-jacques; Picard, Ghislain; Picheral, Marc; Pinczon Du Sel, France; Pogorzelec, Nicole; Probert, Ian; Queguiner, Bernard; Raimbault, Patrick; Ras, Joséphine; Rehm, Eric; Reimer, Erin; Rontani, Jean-françois; Rysgaard, Soren; Saint-béat, Blanche; Sampei, Makoto; Sansoulet, Julie; Schmidt, Sabine; Sempere, Richard; Sevigny, Caroline; Shen, Yuan; Tragin, Margot; Tremblay, Jean-eric; Vaulot, Daniel; Verin, Gauthier; Vivier, Frédéric; Vladoiu, Anda; Whitehead, Jeremy; Babin, Marcel.
The Green Edge initiative was developed to investigate the processes controlling the primary productivity and the fate of organic matter produced during the Arctic phytoplankton spring bloom (PSB) and to determine its role in the ecosystem. Two field campaigns were conducted in 2015 and 2016 at an ice camp located on landfast sea ice southeast of Qikiqtarjuaq Island in Baffin Bay (67.4797N, 63.7895W). During both expeditions, a large suite of physical, chemical and biological variables was measured beneath a consolidated sea ice cover from the surface to the bottom at 360 m depth to better understand the factors driving the PSB. Key variables such as temperature, salinity, radiance, irradiance, nutrient concentrations, chlorophyll-a concentration,...
Tipo: Text
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00598/71000/69266.pdf
Imagem não selecionada

Imprime registro no formato completo
Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing ArchiMer
Massana, Ramon; Gobet, Angelique; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Chambouvet, Aurelie; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Dolan, John R.; Dunthorn, Micah; Edvardsen, Bente; Forn, Irene; Forster, Dominik; Guillou, Laure; Jaillon, Olivier; Kooistra, Wiebe H. C. F.; Logares, Ramiro; Mahe, Frederic; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Probert, Ian; Romac, Sarah; Richards, Thomas; Santini, Sebastien; Shalchian-tabrizi, Kamran; Siano, Raffaele; Simon, Nathalie; Stoeck, Thorsten; Vaulot, Daniel; Zingone, Adriana; De Vargas, Colomban.
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together,...
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00275/38594/37190.pdf
Imagem não selecionada

Imprime registro no formato completo
Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling ArchiMer
Vaulot, Daniel; Lepere, Cecile; Toulza, Eve; De La Iglesia, Rodrigo; Poulain, Julie; Gaboyer, Frederic; Moreau, Herve; Vandepoele, Klaas; Ulloa, Osvaldo; Gavory, Frederick; Piganeau, Gwenael.
Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal...
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00129/23987/21945.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular analyses of protists in long-term observation programmes—current status and future perspectives ArchiMer
Stern, Rowena; Kraberg, Alexandra; Bresnan, Eileen; Kooistra, Wiebe H. C. F.; Lovejoy, Connie; Montresor, Marina; Moran, Xose Anxelu G.; Not, Fabrice; Salas, Rafael; Siano, Raffaele; Vaulot, Daniel; Amaral-zettler, Linda; Zingone, Adriana; Metfies, Katja.
Protists (microbial eukaryotes) are diverse, major components of marine ecosystems, and are fundamental to ecosystem services. In the last 10 years, molecular studies have highlighted substantial novel diversity in marine systems including sequences with no taxonomic context. At the same time, many known protists remain without a DNA identity. Since the majority of pelagic protists are too small to identify by light microscopy, most are neither comprehensively or regularly taken into account, particularly in Long-term Ecological Research Sites. This potentially undermines the quality of research and the accuracy of predictions about biological species shifts in a changing environment. The ICES Working Group for Phytoplankton and Microbial Ecology conducted...
Tipo: Text Palavras-chave: Protists; Long-term Ecological Research Station; Molecular; Time-series; Questionnaire; Literature survey.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00455/56708/58470.pdf
Imagem não selecionada

Imprime registro no formato completo
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex ArchiMer
Balzano, Sergio; Percopo, Isabella; Siano, Raffaele; Gourvil, Priscillia; Chanoine, Melanie; Marie, Dominique; Vaulot, Daniel; Sarno, Diana.
Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA,...
Tipo: Text Palavras-chave: Biogeography; ITS; ITS2 secondary structure; LSU; Morphology; Phylogeny; Polar diatoms; SSU.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00356/46718/46584.pdf
Imagem não selecionada

Imprime registro no formato completo
The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy ArchiMer
Guillou, Laure; Bachar, Dipankar; Audic, S; Bass, David; Berney, Cedric; Bittner, Lucie; Boutte, Christophe; Burgaud, Gaetan; De Vargas, Colomban; Decelle, Johan; Del Campo, Javier; Dolan, John R.; Dunthorn, Micah; Edvardsen, Bente; Holzmann, Maria; Kooistra, Wiebe H. C. F.; Lara, Enrique; Le Bescot, Noan; Logares, Ramiro; Mahe, F; Massana, Ramon; Montresor, Marina; Morard, Raphael; Not, Fabrice; Pawlowski, Jan; Probert, Ian; Sauvadet, Anne-laure; Siano, Raffaele; Stoeck, Thorsten; Vaulot, Daniel; Zimmermann, Pascal; Christen, Richard.
The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes. The Protist Ribosomal Reference database (PR2, http://ssurrna. org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with curated taxonomy. The database mainly consists of nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi and eukaryotic organelles (mitochondrion, plastid and others) are also included because they are useful for the analysis of hightroughput sequencing data sets. Introns and putative chimeric sequences have been also carefully checked. Taxonomic assignation of sequences...
Tipo: Text
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00114/22492/20174.pdf
Registros recuperados: 9
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional