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Registros recuperados: 6
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A unigene catalogue of 5700 expressed genes in cassava Inra
Lopez, C.; Jorge, V.; Piégu, B.; Mba, C.; Cortes, D.; Restrepo, S.; Soto, M.; Laudié, M.; Berger, C.; Cooke, R.; Delseny, M.; Tohme, J.; Verdier, V..
Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv. manihotis. We report here the single pass sequencing of 11 954 cDNA clones from the 5' ends, including 111 from the 3' ends. Cluster analysis permitted the identification of a unigene set of 5700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas ~16% sequences did not show any significant similarity with other proteins present in...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; EXPRESSION DES GENES; RESSOURCE GENOMIQUE; BACTERIOSE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200758ab2534&uri=/notices/prodinra1/2007/04/
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An EST resource for cassava and other species of Euphorbiaceae Inra
Anderson, J.V.; Delseny, M.; Fregene, M.A.; Jorge, V.; Mba, C.; Lopez, C.; Restrepo, S.; Soto, M.; Piégu, B.; Verdier, V.; Cooke, R.; Tohme, J..
Cassava (Manihot esculenta) is a major food staple for nearly 600 million people in Africa, Asia, and Latin America. Major losses in yield result from biotic and abiotic stresses that include diseases such as Cassava Mosaic Disease (CMD) and Cassava Bacterial Blight (CBB), drought, and acid soils. Additional losses also occur from deterioration during the post-harvest storage of roots. To help cassava breeders overcome these obstacles, the scientific community has turned to modern genomics approaches to identify key genetic characteristics associated with resistance to these yield-limiting factors. One approach for developing a genomics program requires the development of ESTs (expressed sequence tags). To date, nearly 23000 ESTs have been developed from...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; SEQUENCE DE GENES; RESSOURCE GENOMIQUE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e657bf31&uri=/notices/prodinra1/2007/04/
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Etude en microscopie electronique a balayage de l'installation de deux souches de Xanthomonas campestris pv manihotis sur feuilles de vitroplants de manioc Inra
Verdier, V.; Schmit, J.; Lemattre, M..
L’installation de 2 souches de Xanthomonas campestris pv manihotis, agressive (XCM17) et non agressive (XCM4), est suivie conjointement par numération et par microscopie électronique à balayage, sur des vitroplants de manioc. Les observations réalisées en microscopie électronique à balayage confirment la dynamique de population des 2 souches et permettent aussi de localiser les bactéries à la surface des tissus. Immédiatement après inoculation, l’aspect et la répartition des bactéries à la surface du limbe sont identiques quelle que soit la souche. Six jours après inoculation, la souche non agressive (XCM4) qui ne se multiplie pas ou peu à la surface du limbe n’entraîne pas d’altération des tissus foliaires. L’observation décèle la présence d’un...
Tipo: Journal Article Palavras-chave: XANTHOMONAS CAMPESTRIS; MICROSCOPIE ELECTRONIQUE BALAYAGE; PHYLLOPLAN; DYNAMIQUE POPULATION; VIRULENCE; SOUCHE.
Ano: 1990 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9100027001018124&uri=/notices/prodinra1/2009/04/
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Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) Inra
Jorge, V.; Fregene, M.A.; Duque, M.C.; Bonierbale, M.W.; Tohme, J.; Verdier, V..
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 2 CM 2177-2) led to the construction of the first molecular genetic map of cassava. To better understand the genetics of resistance to CBB, we evaluated individuals of the F1 cross for CBB resistance by controlled greenhouse inoculations and visually assessed symptoms on days 7, 15, and 30 days after inoculation, using a scale where 0 = no disease and 5 = maximum susceptibility. Five Xam strains were used: CIO-84, CIO-1, CIO-136, CIO-295, and ORST X-27. Area under the...
Tipo: Journal Article Palavras-chave: BACTERIOSE.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007bf8f689c&uri=/notices/prodinra1/2007/05/
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Identification of pathotypes of Xanthomonas axonopodis pv. manihotis in Africa and detection of quantitative trait loci and markers for resistance to bacterial blight of Cassava Inra
Wydra, K.; Zinsou, V.; Jorge, V.; Verdier, V..
Cassava suffers from bacterial blight attack in all growing regions. Control by resistance is unstable due to high genotype–environment interactions. Identifying genes for resistance to African strains of Xanthomonasaxonopodis pv. manihotis can support breeding efforts. Five F1 cassava genotypes deriving from the male parent ‘CM2177-2’ and the female parent ‘TMS30572’ were used to produce 111 individuals by backcrossing to the female parent. In all, 16 genotypes among the mapping population were resistant to stem inoculation by four strains of X. axonopodispv. manihotis from different locations in Africa, and 19 groups with differential reactions to the four strains were identified, suggesting that the strains represent different pathotypes. Four genotypes...
Tipo: Journal Article Palavras-chave: PATHOTYPE; BACTERIOSE DETECTION DE QTL.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076c4dbb22&uri=/notices/prodinra1/2007/06/
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Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis-cassava interaction Inra
Verdier, V.; Restrepo, S.; Mosquera, G.; Jorge, V.; Lopez, C..
Cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis (Xam), is a widespread disease that affects cassava (Manihot esculenta Crantz). Studies on the pathogen population structure, pathogen diagnosis, identification and expression of plant genes involved in resistance have been carried out. Different molecular techniques were developed to assess the genetic diversity among the Xampopulations. Characterization of Xam population dynamics over time had enable us to determine the different factors that are associated with resistance breakdown and those that influence the genetic structure or virulence phenotypes of the pathogen’s population. Methods for detecting the pathogen in vegetative planting materials and true seeds were developed and...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; EXPRESSION DES GENES; BACTERIOSE; ADNC; GENE CANDIDAT; DETECTION DE QTL.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073f223d29&uri=/notices/prodinra1/2007/04/
Registros recuperados: 6
Primeira ... 1 ... Última
 

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