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Registros recuperados: 6
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Absence of Sperm Factors as in the Parthenogenesis Does Not Interfere on BovineEmbryo Sensitiveness to Heat Shock at Pre-Implantation Stage. Repositório Alice
CAMARGO, L. S. de A.; PALUDO, F.; PEREIRA, M. M.; WOHLRES-VIANA, S.; GIOSO, M. M.; CARVALHO, B. C. de; QUINTAO, C. C. R.; VIANA, J. H. M..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Animal reproduction; Embryo thermo-tolerance; Heat shock.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035680
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Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows. Repositório Alice
CAMARGOS, A.; WOHLRES-VIANA, S.; COSTA, I. F.; CAMARGO, L. S. de A.; FERREIRA, J. C.; RAMOS, A. A.; OBA, E..
ABSTRACT The present study investigated the hormonal profile and expression of prostaglandin F2? (PGF2?), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty HolsteinZebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2? metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P<0.05) but no...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: PTGFR; ESRI; Progesterone; Estradiol; RT-PCR.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085645
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113387
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
Abstract This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro&#8208;fertilized or activated with ionomycin and cultured in vitro for 192 hr post&#8208;in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav&#8208;age at 48 hpia. Heat shock had a negative impact (p...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118469
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Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. Repositório Alice
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M..
bitstream/item/195183/1/jbra-23-01-0007.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: In vivo maturation; MRNA; Ovum pick-up; Gene expression.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107759
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Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle. Repositório Alice
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; REIS, D. R. de L.; FERNANDES, L. E.; PEIXOTO, M. G. C. D.; MACHADO, M. A.; VIANA, J. H. M..
Abstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: LHCGR isoforms; Reproduction; Zebu.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1064058
Registros recuperados: 6
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