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Association between molecular markers linked to the Leptin gene and weight gain in postpartum beef cows Ciência Rural
Almeida,Sabrina Esteves Matos; Almeida,Erik Amazonas de; Terra,Gustavo; Neves,Jairo Pereira; Gonçalves,Paulo Bayard Dias; Weimer,Tania de Azevedo.
Three short tandem repeats (STRs), BMS1074, BM1500, IDVGA-51, and three single nucleotide polymorphisms (SNPs), LEPSau3AI (A/B), LEPSau3AI (+/-) and LEPKpn2I linked to the LEP gene were investigated to verify associations with productive performance in postpartum cows of two beef cattle breeds, Aberdeen Angus (AA, n=98) and Charolais (C, n=83). After polymerase chain reaction, STRs were analyzed by vertical electrophoresis and SNPs in agarose gel after endonucleases cleavage. In AA herd 79% of BMS1074*151 carriers had a lower average daily weight gain (ADG) when compared with the population mean daily weight gain (103g), while 62% of BMS1074*151 non-carriers presented a higher ADG (P<0.01); AA animals with at least one BMS1074*151 allele showed a ADG...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptin; STRs; SNPs; Beef cattle; Weight gain; Post-partum.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100033
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BIOCHEMICAL POLYMORPHISMS IN SHEEP AND THEIR POTENTIAL USE FOR PATERNITY TESTS Ciência Rural
Henkes,Luiz Ernani; Weimer,Tania de Azevedo; Moraes,José Carlos Ferrugem de.
The genetic variability of 22 protein loci was investigated in two sheep flocks: 22 females Romney Marsh and 124 animals derived from crossbreeding between Romney Marsh and Merino Booroola, reared by the Brazilian Agricultural Research Corporation (Empresa Brasileira de Pesquisa Agropecuária -EMBRAPA, Bagé, RS, Brazil). Eight loci were polymorphic; the others showed no variation. The usefulness of the eight plymorphic systems (Cat, DIA I, EP-1, EsA, HbB, ME, Tf, and X Prot.) in parentage tests was analyzed. The probability to find two random identical animals in each breed was estimated as 1:1000. The efficiency of these proteins for exclusion of one of two possible sires in parentage tests was about 77% both for Romney Marsh and Romney/ Booroola flocks....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sheep; Genetic potymorphisms; Paternity tests.
Ano: 1994 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300023
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Diversity of microsatellites linked to the FSHbeta gene, their usefulness for individual identification and association with reproductive performance Ciência Rural
Duarte,Letícia Becker Homrich; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo.
The genetic diversity of three microsatellites (ILSTS027, MBO22, BM4325) mapped on the bovine chromosome 15 and linked to the follicle-stimulating hormone beta gene (FSHß) was investigated in cows of a Brangus Ibagé herd and the efficiency of these markers for individual identification and parentage control was estimated. Possible associations between each molecular marker and cow reproductive performance were also analyzed. Six alleles were detected in BM4325 and ILSTS027 and 12 in MB022, the most frequent being BM4325*101, BM4325*103, ILSTS027*169 and MB022*229. Polymorphic information content ranged from 0.58 to 0.88 while expected heterozygosity ranged from 65% to 89%, with an average mean value of 77%. Although only three markers were studied, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Bovine genetic diversity; Individual identification; Reproductive performance; Marker-assisted selection.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100023
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Genetic characterization of a brangus-ibage cattle population: biochemical polymorphisms and reproductive efficiency Ciência Rural
Henkes,Luiz Ernani; Papadopolis,Lídia Gonzalez; Steigleder,Clara Sabina; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo.
Biochemical techniques were used to investigate the genetic variability in a Brangus-Ibage population by determining allele frequencies of 18 blood protein systems: Hemogloin beta-Chain (Hb), Albumin (Alb), Amylase (Am), Transferrin (Tf), Carbonic Anhydrase (CA), Ceruloplasmin (Cp), Malic Enzyme (ME), Diaphorase I and II (Dia I and Dia II), Slow Alpha 2 Macroglobulin (Ap), Acid Phosphatase (ACP), Esterase B and D (EstB and EstD), Phosphogluconate Dehydrogenase (PGD), Glucose-6-Phosphate Dehydrogenase (G-6-PD), Glucose-Phosphate-Isomerase (GPI), Superoxide Dismutase (SOD) and Glyoxalase I (GLO). The percentage of polymorphic loci were estimated at 0.27, the mean number of alleles was 1.33 and the mean heterozygosity was 0.07. There was a good agreement...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brangus-Ibage cattle; Genetic characterization; Reproductive efficiency.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000500010
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Genetic diversity in a Brazilian bovine herd based on four microsatellite loci Genet. Mol. Biol.
Almeida,Sabrina E. Matos; Machado,Márcia S.N.; Steigleder,Clara Sabina; Gama,Cleonice L.; Hutz,Mara Helena; Henkes,Luiz Ernani; Moraes,José Carlos F.; Weimer,Tania de Azevedo.
Microsatellites or short tandem repeats (STRs), DNA markers relatively abundant in the genome, have a high degree of polymorphism and therefore great potential for characterizing populations. The present study estimates genetic variability in a set of four microsatellites (BMS3013, BMS3004, HEL10 and TGLA122) in a Brazilian hybrid bovine breed (5/8 Aberdeen Angus x 3/8 Nelore). The objectives were to determine the effect of crossbreeding and selection in these animals' genetic diversity as well as to discover the herd's genetic relationship with that of other breeds. Low diversity was verified in BMS3013 and high diversity was detected in BMS3004, HEL10 and TGLA122. Two alleles in TGLA122 are described here for the first time (TGLA122*155 and TGLA122*163)....
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200018
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Genetic polymorphisms at the leptin receptor gene in three beef cattle breeds Genet. Mol. Biol.
Almeida,Sabrina E.M.; Santos,Luciana B.S.; Passos,Daniel T.; Corbellini,Ângela O.; Lopes,Beatriz M.T.; Kirst,Cláudia; Terra,Gustavo; Neves,Jairo P.; Gonçalves,Paulo B.D.; Moraes,José C.F.; Weimer,Tania de Azevedo.
The genetic diversity of a single nucleotide polymorphism (SNP) at the exon 20 (T945M) of the leptin receptor gene (LEPR) and of three short tandem repeats (STRs BM7225, BMS694, and BMS2145) linked to LEPR was investigated in three beef cattle herds (Brangus Ibagé, Charolais, and Aberdeen Angus). A cheap and effective new method to analyze the T945M polymorphism in cattle populations was developed and the possible role of these polymorphisms in reproduction and weight gain of postpartum cows was evaluated. High levels of genetic diversity were observed with the average heterozygosity of STRs ranging from 0.71 to 0.81. No significant association was detected between LEPR markers and reproductive parameters or daily weight gain. These negative results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Genetic diversity; Cattle production; LEPR gene polymorphisms.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400013
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Identification of molecular markers on bovine chromosome 18 associated to calving interval in a Brangus-Ibagé cattle herd Ciência Rural
Weimer,Tania de Azevedo; Steigleder,Clara Sabina; Machado,Márcia Silveira; Almeida,Sabrina Esteves Matos; Oliveira,João Francisco Coelho; Moraes,José Carlos Ferrugem; Henkes,Luis Ernani.
In the detection phase of a bovine marker assisted selection program, this paper investigated the genetic variability of three microsatellites on the chromosome 18 (BTA 18). The possible associations between genotypes or alleles of these markers versus weight at first calving and a lifetime calving interval (as indicators of reproductive performance) were evaluated in a beef cattle herd (5/8 Aberdeen Angus x 3/8 Nelore). Eleven alleles were detected in TGLA227 and ILSTS002 and three in BMS3004, the most frequent being TGLA227*79, ILSTS002*133, ILSTS002*135 and BMS3004*129. Polymorphic information content ranged from 0.41 to 0.84, while heterozygosity ranged from 49% to 86%, with an average value of 77%. The association analyses performed between genotype...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Reproduction; Marker assisted selection; BTA 18.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500050
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Isolamento de Salmonella Braenderup em arara-azul (Anodorhynchus hyacinthinus) Ciência Rural
Allgayer,Mariangela Costa; Oliveira,Sérgio José de; Mottin,Vanessa Daniele; Loiko,Márcia Regina; Abilleira,Fernanda; Guedes,Neiva Maria Robaldo; Passos,Daniel Thompsen; Weimer,Tania de Azevedo.
O monitoramento sanitário de populações de arara-azul (Anodorhynchus hyacinthinus) de vida livre visa a permitir ajustes de manejo em ambiente natural alterado. Para avaliar a ocorrência de Salmonella spp. em filhotes dessa espécie, foram coletados swabs de cloaca no Pantanal de Miranda, Mato Grosso do Sul (MS). Uma colônia morfológica e bioquimicamente compatível com Salmonella spp. foi isolada e sorotipada como Salmonella Braenderup. Devido ao alto potencial zoonótico desse microrganismo, é importante o controle sanitário de psitacídeos em vida livre. Na literatura pesquisada não foi encontrado relato sobre o isolamento dessa bactéria em arara-azul, tanto em vida livre, como em cativeiro.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella spp.; Anodorhynchus hyacinthinus de vida livre; Microbiota cloacal.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000800044
Registros recuperados: 8
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