SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Análise de associação genômica ampla (GWAS) para tolerância à seca em feijoeiro comum.

BORBA, T. C. de O.; ZUCCHI, M. I.; BRONDANI, R. V.; RANGEL, P. H. N.; MAGALHÃES, M. R.; BRONDANI, C..

Este trabalho teve como objetivo avaliar a variabilidade genética através de marcadores moleculares SSR em uma amostra de variedades tradicionais oriundas de coletas, conduzidas entre os anos de 1978 e 1996, em lavouras de pequenos agricultores no Brasil.

Tipo: Artigo em anais de congresso (ALICE)

Palavras-chave: Coleção nuclear; Arroz; Oryza sativa; Recurso genético; Marcador molecular.

Ano: 2003

URL: http://www.alice.cnptia.embrapa.br/handle/doc/936126

VALDISSER, P. A. M. R.; PEREIRA, W. J.; MORAIS JUNIOR, O. P. de; MENDONÇA, J. A.; SARTORI, D. E. L.; LIMA, LUANN V. V. O.; BORBA, T. C. de O.; BRONDANI, C.; ZUCCHI, M. I.; VIANELLO, R. P..

O objetivo deste estudo foi identificar locos genéticos associados à tolerância à seca através da análise de associação genômica ampla (GWAS).

Caracterização da diversidade do germoplasma de mamona (ricinus communis l.) utilizando marcadores microssatélites.

Palavras-chave: Feijão; Resistência a seca; Deficiencia hídrica; Phaseolus vulgaris.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078828

Caracterização do germoplasma de feijoeiro comum com base em marcadores DArt-Seq.

BAJAY, M. M.; ZUCCHI, M. I.; NOBREGA, M. B. de M.; KIIHL, T. A. M.; ZANOTTO, M. D.; PINHEIRO, J. B..

2010

Tipo: Artigo em anais de congresso (ALICE)

Palavras-chave: Diversidade genética; Marcadores microssatélites; Germoplasma; Mamona.

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/handle/doc/858161

VALDISSER, P. A. M. R.; ZUCCHI, M. I.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P..

Esse estudo teve como objetivo identificar e caracterizar SNPs em um conjunto de acessos de feijão com alta diversidade genética através da metodologia de GbS via DArT-Seq.

Development of microsatellite markers in Guineagrass (Panicum maximum Jacq.) and their transferability to other tropical forage grass species.

Palavras-chave: GBS; SNPs; Feijão; Phaseolus vulgaris; Gemoplasma; Melhoramento genético vegetal.

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1022480

SOUSA, A. C. B.; JUNGMANN, L.; CAMPOS, T. de; SFORÇA, D. A.; BOAVENTURA, L. R.; SILVA, G. M. B.; ZUCCHI, M. I.; JANK, L.; SOUZA, A. P..

The Guineagrass (Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge and tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, and employed in estimating genetic relationships among 25 P. maximum genotypes selected from a Brazilian germplasm collection. In addition, they were tested for cross-species amplification in four other forage grass species. The number of alleles observed for each locus ranged from 4 to 12 (average 6.7). The values of polymorphism information content (PIC) varied from 0.41 to 0.83 (average 0.61) and the discriminating power (D) ranged from 0.53 to 0.98 (average 0.72). Cross-amplification demonstrated the...

Diversidade genética entre populações de coqueiro gigante do Brasil por meio de marcadores microssatélites (SSR).

Palavras-chave: Capim Tanzânia; Guineagrass; Marcador microssatélite; Amplificação cruzada; Melhoramento genético vegetal; Gramínea forrageira; Capim Colonião; Panicum maximum; Seleção genótipa; Variação genética; Marcador genético; Polimorfismo genético; Plant breeding; Forage grasses; Megathyrsus maximus; Genotype; Genetic variation; Genetic markers; Microsatellite repeats; Genetic polymorphism; Fitomejoramiento; Pastos forrajeros; Variación genética; Genotipo; Marcadores genéticos; Repeticiones de microsatélite.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/handle/doc/901385

RIBEIRO, F. E.; BAUDOUIN, L.; LEBRUN, P.; CHAVES, L. J.; BRONDANI, C.; ZUCCHI, M. I.; VENCOSVSKY, R..

O presente trabalho teve por objetivo avaliar a diversidade genética entre e dentro de populações de coqueiro gigante do Brasil por meio de marcadores microssatélites (SSR).

Estrutura genética de populações silvestres de Oryza glumaepatula em três biomas brasileiros utilizando marcadores microssatélites.

Palavras-chave: Estrutura populacional; Variabilidade genética; Coco; Marcador molecular.

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/handle/doc/866761

Infoteca-e

BRONDANI, R. P. V.; RANGEL, P. H. N.; ZUCCHI, M. I.; MAGALHÃES, M. R.; BORBA, T. C. O.; VENCOVSKY, R.; BRONDANI, C..

Oryza glumaepatula, uma das 21 espécies silvestres do gênero Oryza, no qual está incluído o arroz cultivado (O. sativa), constitui uma valiosa fonte doadora de alelos favoráveis para características de interesse agronômico desta espécie. Diversas áreas de ocorrência natural de O. glumaepatula estão sob ameaça de devastação e, desta forma, a implementação de estratégias de conservação destes recursos genéticos a partir de estudos de genética de populações é de extrema relevância. Marcadores moleculares microssatélites, ou SSR, são uma ferramenta útil para investigar questões relacionadas ao sistema de cruzamento, fluxo gênico e estrutura genética das populações naturais de O. glumaepatula. Este trabalho objetivou caracterizar a variabilidade genética e...

Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)

Palavras-chave: Arroz; Oryza glumaepatula; Silvestre; SSR; Conservação; Marcador molecular; Variabilidade genética.

Ano: 2003

URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/212641

Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers.

SOUSA, A. C. B.; GODOY, R.; SFORÇA, D. A.; CAMPOS, T. de; ZUCCHI, M. I.; JANK, L.; SOUZA, A. P. de.

The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...

Genetic diversity in cultivated carioca common beans based on molecular marker analysis.

Palavras-chave: Inferência bayesiana; Bayesian inference; Sofware Structure; Guandu; Cajanus cajan; Variação genética; Marcador genético; Polimorfismo genético; Feijão; Phaseolus vulgaris; Feijão de corda; Vigna unguiculata; Método estatístico; Modelo de simulação; Pigeon peas; Genetic variation; Guandul; Genetic markers; Statistical analysis; Genetic polymorphism; Beans; Computer simulation; Variación genética; Marcadores genéticos; Análisis estadístico; Frijoles; Simulación por computadora.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/handle/doc/902585

PERSEGUINI, J. M. C. K.; CHIORATTO, A. F.; ZUCCHI, M. I.; COLOMBO, C. A.; CARBONELL, S. A. M.; MONDEGO, J. M. C.; GAZAFFI, R.; GARCIA, A. A. F.; CAMPOS, T. de; SOUZA, A. P. de; RUBIANO, L. B..

A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger?s modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were...

Genetic diversity in soybean germplasm identified by SSR and EST-SSR markers.

Palavras-chave: Marcador microssatélite; Feijão carioca; Melhoramento genético vegetal; Feijão; Phaseolus vulgaris; Variação genética; Marcador genético; Polimorfismo genético; Plant breeding; Beans; Genetic variation; Genetic markers; Microsatellite repeats; Genetic polymorphism; Fitomejoramiento; Frijoles; Variación genética; Marcadores genéticos; Repeticiones de microsatélite.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/handle/doc/902577

MULATO, B. M.; MÖLLER, M.; ZUCCHI, M. I.; QUECINI, V.; PINHEIRO, J. B..

Os objetivos deste trabalho foram avaliar a diversidade genética de 79 acessos de soja de diferentes regiões do mundo, agrupá-los de acordo com a similaridade e testar a correlação entre os dois tipos de marcadores utilizados. Foram utilizados marcadores microssatélites genômicos (SSR) e funcionais (EST-SSR). Trinta pares de primers SSR foram selecionados (20 genômicos e 10 EST-SSR) de acordo com sua distribuição nos 20 grupos de ligação da soja, com sua unidade de repetição trinucleotídica e com seu conteúdo de informação polimórfica. Todos os lócus analisados foram polimórficos, e 259 alelos foram encontrados. O número de alelos por lócus variou entre 2?21, com média de 8,63. Os acessos possuem uma quantidade significativa de alelos raros, sendo os...

Genetic diversity in soybean germplasm identified by SSR and EST-SSR markers.

Palavras-chave: Diversidade genética; Sequenciamento genético; Conteúdo de informação polimórfica; Melhoramento genético; Soja; Biologia molecular; Germoplasma; Variedade; Marcador Molecular.

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/865548

MULATO, B. M.; MOLLER, M.; ZUCCHI, M. I.; QUECINI, V.; PINHEIRO, J. B..

Abstract ? The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2?21, with an average of 8.63. The accessions exhibit...

Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-Genotype core collection.

Palavras-chave: Molecular marker; Plant germplasm; Polymorphism information content; Glycine max; Plant breeding.

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/handle/doc/858527

SOUZA, L. M. de; LE GUEN, V.; CERQUEIRA-SILVA, C. B. M.; SILVA, C. C.; MANTELLO, C. C.; CONSON, A. R. O.; VIANNA, J. P. G.; ZUCCHI, M. I.; SCALOPPI JUNIOR, E. J.; FIALHO, J. de F.; MORAES, M. L. T. de; GONÇALVES, P. de S.; SOUZA, A. P. de.

Abstract - The rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] is the only plant species worldwide that is cultivated for the commercial production of natural rubber. This study describes the genetic diversity of the Hevea spp. complex that is available in the main ex situ collections of South America, including Amazonian populations that have never been previously described. Genetic data were analyzed to determine the genetic structure of the wild populations, quantify the allelic diversity and suggest the composition of a core collection to capture the maximum genetic diversity within a minimal sample size. A total of 1,117 accessions were genotyped with 13 microsatellite markers. We identified a total of 408 alleles, 319 of which...

Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers.

Palavras-chave: Hevea brasiliensis; Natural rubber; Estrutura genética; Genetic structure.

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035994

ZUCCHI, M. I.; BRONDANI, R. P. V.; PINHEIRO, J. B.; CHAVES, L. J.; COELHO, A. S. G.; VENCOVSKY, R..

The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including...

Genetic variability of garlic accessions as revealed by agro-morphological traits evaluated under different environments.

Palavras-chave: Genetic diversity; Tropical tree; SSR; Eugenia Dysenterica; Cagaita; Cerrado; Variação Genética; Marcador Molecular; Myrtaceae.

Ano: 2003

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122034

HOOGERHEIDE, E. S. S.; AZEVEDO FILHO, J. A.; VENCOVSKY, R.; ZUCCHI, M. I.; ZAGO, B. W.; PINHEIRO, B. J..

The cultivated garlic (Allium sativum L.) displays a wide phenotypic diversity, which is derived from natural mutations and phenotypic plasticity, due to dependence on soil type, moisture, latitude, altitude and cultural practices, leading to a large number of cultivars. This study aimed to evaluate the genetic variability shown by 63 garlic accessions belonging to Instituto Agronômico de Campinas and the Escola Superior de Agricultura ?Luiz de Queiroz? germplasm collections. We evaluated ten quantitative characters in experimental trials conducted under two localities of the State of São Paulo: Monte Alegre do Sul and Piracicaba, during the agricultural year of 2007, in a randomized blocks design with five replications. The Mahalanobis distance was used...

Genetic variation in polyploid forage grass: assessing the molecular genetic variability in the Paspalum genus.

Palavras-chave: Allium sativum; Cluster analysis; Multivariate analysis; Genetic variation.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085920

CIDADE, F. W.; VIGNA, B. B. Z.; SOUZA, F. H. D. de; DALL´AGNOL, M.; ZUCCHI, M. I.; SOUZA-CHIES, T. T. de; SOUZA, A. P..

bitstream/item/84707/1/PROCI-2013.00034.pdf

Genomic diversity is similar between Atlantic Forest restorations and natural remnants for the native tree Casearia sylvestris Sw.

Palavras-chave: Cross-species; Genetic diversity; Germaplasm evaluation.

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/960437

VIANA, J. P. G.; SIQUEIRA, M. V. B. M.; ARAUJO, F. L.; GRANDO, C.; SUJII, P. S.; SILVESTRE, E. de A.; NOVELLO, M.; PINHEIRO, J. B.; CAVALLARI, M. M.; BRANCALION, P. H. S.; RODRIGUES, R. R.; SOUZA, A. P. de; CATCHEN, J.; ZUCCHI, M. I..

The primary focus of tropical forest restoration has been the recovery of forest structure and tree taxonomic diversity, with limited attention given to genetic conservation. Populations reintroduced through restoration plantings may have low genetic diversity and be genetically structured due to founder effects and genetic drift, which limit the potential of restoration to recover ecologically resilient plant communities. Here, we studied the genetic diversity, genetic structure and differentiation using single nucleotide polymorphisms (SNP) markers between restored and natural populations of the native tree Casearia sylvestris in the Atlantic Forest of Brazil. We sampled leaves from approximately 24 adult individuals in each of the study sites: two...

High genetic diversity among and within bitter manioc varieties cultivated in different soil types in Central Amazonia.

Palavras-chave: SNP; Single nucleotide polymorphisms; Floresta Tropical; Casearia Sylvestris; Tropical forests.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101157

PEREIRA, A. A.; PERONI, N.; CAVALLARI, M. M.; LEMES, M. R.; ZUCCHI, M. I.; CLEMENT, C. R..

Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols. We tested this hypothesis evaluating the intra-varietal genetic diversity and the genetic relationships among manioc varieties commonly cultivated in Oxisols, ADE and floodplain soils. Genetic results did not agree...

High genetic diversity within and among bitter cassava cultivated in three soil types in Central Amazonia.

Palavras-chave: Manihot esculenta; Microsatellites; Amazonian dark earths.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1075693

ALVES-PEREIRA, A.; PERONI, N.; CAVALLARI, M. M.; PINHEIRO, J. B.; LEMES, M. R.; CLEMENT, C. R.; ZUCCHI, M. I..

Bitter cassava is an important food crop that was domesticated in Amazonia. Although it is exclusively propagated by stem cuttings, cassava retained its ability of sexual reproduction. The occurrence and incorporation of sexual plants into the stock of clonal varieties contributes to the high genetic diversity observed within the crop. Despite being well adapted to nutrient deprived soils of Amazonia, ethnobotanical observations showed that communities of smallholder farmers along the middle Madeira River, in Central Amazonia, also cultivate cassava in the highly fertile soils of the floodplains and Amazonian dark earths (ADE). These farmers grow different sets of varieties in each soil type, which may also contribute to the maintenance of high levels of...

Identification of stylosanthes guianensis varieties using molecular genetic analysis.

Palavras-chave: Reprodução; Diversidade genética; Amazônia; Mandioca; Cassava; Bitter cassava.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/987518

SANTOS-GARCIA, M. O.; KARIA, C. T.; RESENDE, R. M. S.; CHIARI, L.; VIEIRA, M. L. C.; ZUCCHI, M. I..

The botanical classification of Stylosanthes guianensis is controversial, and few studies have used molecular markers to analyse this species. We used microsatellite markers to study the genetic diversity and population structure of S. guianensis and compare our results with the current infraspecific botanical classification. A representative sample from the S. guianensis Brazilian germplasm collection (150 accessions) was analysed using 20 microsatellite loci. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign accessions into clusters.

In-depth genome characterization of a Brazilian common bean core collection using DArTseq high-density SNP genotyping.

Palavras-chave: Stylosanthes guianensis; Cerrado; Marcador molecular; Genética vegetal; Leguminosa.

Ano: 2012

URL: http://www.alice.cnptia.embrapa.br/handle/doc/937141

VALDISSER, P. A. M. R.; PEREIRA, W. J.; ALMEIDA FILHO, J. E.; MÜLLER, B. S. F.; COELHO, G. R. C.; MENEZES, I. P. P. de; VIANNA, J. P. G.; ZUCCHI, M. I.; LANNA, A. C.; COELHO, A. S. G.; OLIVEIRA, J. P. de; MORAES, A. da C.; BRONDANI, C.; VIANELLO, R. P..

Background: Common bean is a legume of social and nutritional importance as a food crop, cultivated worldwide especially in developing countries, accounting for an important source of income for small farmers. The availability of the complete sequences of the two common bean genomes has dramatically accelerated and has enabled new experimental strategies to be applied for genetic research. DArTseq has been widely used as a method of SNP genotyping allowing comprehensive genome coverage with genetic applications in common bean breeding programs. Results: Using this technology, 6286 SNPs (1 SNP/86.5 Kbp) were genotyped in genic (43.3%) and non-genic regions (56. 7%). Genetic subdivision associated to the common bean gene pools (K = 2) and related to grain...