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12x coverage 454/Sanger Hybrid Assembly of Cucumber (Cucumis sativus L. cv.Borszczagovski) genome - the most efficient way to begin post genomic era Nature Precedings
Rafał Wóycicki; Justyna Witkowicz; Magdalena Pawełkowicz; Ewa Siedlecka; Wojciech Gutman; Wojciech Pląder; Anna Seroczyńska; Mieczysław Śmiech; Katarzyna Niemirowicz-Szczytt; Stanisław Karpiński; Stefan Malepszy; Zbigniew Przybecki.
New sequencing technologies make it possible to achieve genome sequence fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects. 
We present here approaches undertaken on the way to the draft assembly the cucumber (Cucumis sativus L. cv. Borszczagowski) genome done with the use of 8x unpaired and 4x paired (3 Kbp) pyrosequenced 454 XLR Titanium reads, together BAC library ends fragments (12,7x physical coverage). Two different assembly approaches, namely Celera and Celera/Arachne were finally used. The Celera result was 15’196 contigs of 197 Mbp length and N50 27’086 bp and 4’157 scaffolds of 224 Mbp length...
Tipo: Poster Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/5466/version/1
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Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes Nature Precedings
Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawełkowicz; Rafał Wóycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. 
To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo: Manuscript Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/5465/version/1
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Applying of novel subtraction method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes Nature Precedings
Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawełkowicz; Rafał Wóycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. 
To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo: Manuscript Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5465/version/2
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BRAGOMAP - a new Perl script for high throughoutput blast results analysis including GO and MapMan automatic annotations Nature Precedings
Rafal Woycicki; Wojciech Gutman; Zbigniew Przybecki.
Analyzing of sequences similarities is the first and most important method used to find out the function of unknown nucleotides. Searching of homologs should be done carefully not to loose any important ones. Having thousands of results from various long-read sequencing projects (ie. differentially expressed tags, genomic polymorphons or BAC ends), the by-hand ability to retrieve interesting (to our goal) similarities in hundreds of Blast results decreases rapidly. Decreasing the number of retrieved sequences by giving more stringency in e-value threshold or displaying less results could lead to false deductions. Functional genomics, proteomics and metabolomics could give us answers to the role of nucleotide sequences. It makes the need to annotate as much...
Tipo: Poster Palavras-chave: Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3900/version/1
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Pyrosequencing/Sanger Plant Genome Assembly (Limitations, Problems And Solutions) - On The Way To Cucumber (Cucumis sativus L. cv. Borszczagowski) Draft Genome Sequence Publishing Nature Precedings
Rafal Woycicki; Zbigniew Przybecki.
Genome sequencing give us an opportunity to broaden the range and improve the quality of omics studies. New sequencing technologies make it possible to achieve the sequencing reads fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects.
We focus here on the wide range of approaches undertaken to assemble the draft cucumber (Cucumis sativus L. cv. Borszczagowski) genome done by using 12x coverage of pyrosequencing Titanium chemistry shotgun paired and unpaired reads combined with Sanger reads.
Tipo: Poster Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4630/version/1
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The Differences Between Products Of Gene Expression In Male, Female And Hermaphrodite Cucumber Floral Buds (Cucumis Sativus L.) Nature Precedings
Magdalena Pawelkowicz; Ewa Siedlecka; Zbigniew Przybecki.
Cucumber is a species in which sex expression has been extensively studied. Sexual differentiation is controlled by genotypic and environmental factors. The main genes responsible for sex determination have been described but the mechanism of their action remains unexplained. In this study we attempted to find cDNA clones which can be connected with sex differentiation and flower development in cucumber. 
Two pairs of nearly isogenic lines: GY-3 (gynoecious – FFMMGG) and HGY-3 (hermaphrodite - FFmmGG), B10 (monoecious - ffMMGG) and 2gg (gynoecious - FFMMgg) were used to search for differences in gene expression in young (1 – 2mm) cucumber floral buds.
In order to obtain differentially expressed cDNA...
Tipo: Poster Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5605/version/1
Registros recuperados: 6
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