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Registros recuperados: 4
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A three-phase epidemiological study of short and long sleepers in a middle-aged Chinese population: prevalence and characteristics BJMBR
Hao,Y.L.; Zhang,B.; Jia,F.J.; Li,X.L.; Tang,Y.; Ren,Y.Z.; Liu,W.H..
Epidemiological studies of short and long sleepers have not been conducted previously. We collected socioeconomic, psychological, and polysomnographic characteristics of 6501 parents (3252 men and 3249 women) of 4036 primary school children in Guangzhou city. The study data were collected in three phases. The overall prevalence of short (5 h or less) and long (10 h or more) sleep duration was 0.52 and 0.64%, respectively. Long sleepers had higher Eysenck Personality Questionnaire neuroticism scores [odds ratio (OR)=1.224, 95% confidence interval (CI)=1.047-1.409] and lower education levels (OR=0.740, 95%CI=0.631-0.849) than short sleepers. In the polysomnographic assessment, short, long, and normal sleepers (7-8 h) shared similar durations of Stage 3 sleep...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Short sleeper; Long sleeper; Prevalence; Characteristics; Chinese.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200157
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Circular RNA hsa_circ_0000523 regulates the proliferation and apoptosis of colorectal cancer cells as miRNA sponge BJMBR
Jin,Y.; Yu,L.L.; Zhang,B.; Liu,C.F.; Chen,Y..
Among the novel class of endogenous long non-coding RNAs, circular RNA (circRNA) is known as a key regulator in the development and progression of different cancers. Its function and mechanism in the tumorigenesis of colorectal cancer, however, has not been well studied. This study thus aimed to investigate potential regulation of colorectal cancer by circRNAs and the corresponding regulatory mechanism. We demonstrated that the expression of circRNA hsa_circ_0000523 (also known as circ_006229) was down-regulated in different colorectal cancer cell lines. It was also found that interference of hsa_circ_0000523 induced proliferation and suppressed apoptosis of colorectal cancer cells, the proliferation rate of which was reduced by the overexpression of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Circular RNA hsa_circ_0000523; Colorectal cancer; Proliferation; Sponge; Interference.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200611
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Retinoic acid protects from experimental cerebral infarction by upregulating GAP-43 expression BJMBR
Li,Y.; Gao,X.; Wang,Q.; Yang,Y.; Liu,H.; Zhang,B.; Li,L..
The aim of this study was to investigate whether exogenous retinoic acid (RA) can upregulate the mRNA and protein expression of growth-associated protein 43 (GAP-43), thereby promoting brain functional recovery in a rat distal middle cerebral artery occlusion (MCAO) model of ischemia. A total of 216 male Sprague Dawley rats weighing 300–320 g were divided into 3 groups: sham-operated group, MCAO+vehicle group and MCAO+RA group. Focal cortical infarction was induced with a distal MCAO model. The expression of GAP-43 mRNA and protein in the ipsilateral perifocal region was assessed using qPCR and immunocytochemistry at 1, 3, 7, 14, 21, and 28 days after distal MCAO. In addition, an intraperitoneal injection of RA was given 12 h before MCAO and continued...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Focal cerebral infarction; Growth associate protein 43; Retinoic acid; Nerve regeneration.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400606
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Tissue expression and subcellular localization of Mipu1, a novel myocardial ischemia-related gene BJMBR
Wang,G.; Zuo,X.; Jiang,L.; Wang,K.; Wei,X.; Zhang,B.; Xiao,X..
Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1), a novel zinc finger protein, was originally cloned using bioinformatic analysis and 5' RACE technology of rat heart after a transient myocardial ischemia/reperfusion procedure in our laboratory. In order to investigate the functions of Mipu1, the recombinant prokaryotic expression vector pQE31-Mipu1 was constructed and transformed into Escherichia coli M15(pREP4), and Mipu1-6His fusion protein was expressed and purified. The identity of the purified protein was confirmed by mass spectrometry. The molecular mass of the Mipu1 protein was 70.03779 kDa. The fusion protein was intracutaneously injected to immunize New Zealand rabbits to produce a polyclonal antibody. The antibody titer was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mipu1; Purification; Expression; Polyclonal antibody.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100007
Registros recuperados: 4
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