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Characterization and expression analysis of WOX2 homeodomain transcription factor in Aegilops tauschii Genet. Mol. Biol.
Zhao,Shan; Jiang,Qian-Tao; Ma,Jian; Wang,Ji-Rui; Liu,Ya-Xi; Chen,Guo-Yue; Qi,Peng-Fei; Pu,Zhi-En; Lu,Zhen-Xiang; Zheng,You-Liang; Wei,Yu-Ming.
The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: WOX2; Aegilops tauschii; Phylogenetic analysis; Real-time quantitative PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100079
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