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Registros recuperados: 119
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18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES BJM
Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M..
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
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A COMPARATIVE STUDY OF BACTERIOSTATIC ACTIVITY OF SYNTHETIC HYDROXYLATED FLAVONOIDS BJM
Olivella,Mónica S.; Zarelli,Valeria E.P.; Pappano,Nora B.; Debattista,Nora B..
Among other properties, flavonoids present a notable bacteriostatic activity. In this paper, minimal inhibitory concentrations (MICs) of 5,7,4'-trihydroxyflavanone (naringenin), 5,7-dihydroxyflavone and 2',4',4- trihydroxychalcone (isoliquitirigenin) against Staphylococcus aureus ATCC 25 923 were determined and compared to values obtained for other chalcones and flavanones previously investigated. Specific growth rates and MICs were determined by a turbidimetric kinetic method. The observed sequence MICflavanone (inactive) >MIC7-hidroxyflavanone (197.6 µgml-1)>MIC5,7,4'-trihydroxyflavanone (120 µgml-1) showed that the introduction of an electron donating group (-OH) causes an increase in bioactivity. On the other hand, the comparisons...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavanone; Flavone; Chalcone; Bacteriostatic activity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300013
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A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium BJM
Nascimento,Aline E.; Shariá,Allana E. do Nascimento; Lima,Marco Antonio Barbosa de; Campos-Takaki,Galba Maria.
Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipolytica; Carbohydrate cytochemistry; Fungi ultrastructure.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008
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A method for the determination of two Alternaria toxins, alternariol and alternariol monomethyl ether, in tomato products BJM
Motta,Silvana da; Soares,Lucia M. Valente.
Tomatoes, as any soft skinned fruit, are easily susceptible to fungal rot. Alternaria is the genus most frequently encountered in tomatoes. A. alternata and other spp. have been shown to produce the toxins alternariol monomethyl ether (AME) and alternariol (AOH) in tomatoes. A method for determining AME and AOH in tomato products was developed and evaluated. The method involves extraction with methanol, clarification with ammonium sulfate, and partition to chloroform. Quantification was conducted by high performance liquid chromatography with diode array detector (DAD). Average recoveries were 98.7% and 84.1% for AME and AOH, respectively. The quantification limits of the method, defined as the minimum amount that allowed quantification and confirmation by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternaria; Mycotoxins; Alternariol monomethyl ether; Alternariol; Tomatoes.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400015
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A practical method for screening for beta-galactosidase secreting microbial colonies BJM
Barreto,Eriana S.; Silva,Daison O.; Passos,Flavia M. Lopes.
Microbial colonies were replicated on YNB® agar plates overlaid with soft agar containing the glucose-oxidase/peroxidase (BIOTROL®) system. The pink color developed around the colonies was the result of the reaction of the glucose generated by the extracellular hydrolysis of lactose by beta-galactosidase, indicating secretion of this enzyme. This method proved to be very convenient for testing hundreds of colonies grown on agar plates for beta-galactosidase secretion by microbial cells.
Tipo: Info:eu-repo/semantics/other Palavras-chave: BIOTROL®; Beta-galactosidase.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100009
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A suitable procedure to choose antimicrobials as controlling agents in fermentations performed by bacteria BJM
Oliveira,Romulo C.; Gomez,José G.C.; Torres,Bayardo B.; Bueno Netto,Celso L.; Silva,Luiziana F. da.
This work evaluated the influence of nitrofurantoin, erythromycin and streptomycin at 50, 25 and 12,5% of the minimal inhibitory concentration (MIC) on maximum specific growth rate (µmax) and specific polymer accumulation rate (µPHB) of Alcaligenes eutrophus, considered resistant to those antimicrobials. Nitrofurantoin strongly affected µmax even at 50% MIC. Streptomycin moderately affected µmax only at 50%MIC. Nitrofurantoin showed the most harmful effect on µPHB when 50% MIC was applied and erythromycin was not harmful.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Antimicrobial susceptibility; MIC; PHB; Alcaligenes eutrophus; Ralstonia eutropha.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200004
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Action of peracetic acid on Escherichia coli and Staphylococcus aureus in suspension or settled on stainless steel surfaces BJM
Kunigk,Leo; Almeida,Maria C.B..
The efficiency of a commercial peracetic acid sanitizer on destruction of Staphylococcus aureus and Escherichia coli was evaluated using two distinct methods. The first method is the AOAC suspension test and the second is a method proposed by one of the authors in which the microbial cells are settled on a stainless steel surface and then treated with the sanitizer. The results showed that when in suspension S. aureus was more resistant to the sanitizer than E. coli. When S. aureus was settled on the stainless steel surface, the contact time between the sanitizer and the microorganisms to attain a 6.5 log reduction in the number of viable cells was three times greater than when the cells were in suspension.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peracetic acid; Staphylococcus aureus; Escherichia coli; Stainless steel surface; Sanitizers.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100009
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Activity of the aqueous extract from Polymnia sonchifolia leaves on growth and production of aflatoxin B1 by Aspergillus flavus BJM
Pinto,Marina M.; Gonçalez,Edlayne; Rossi,Maria H.; Felício,Joana D.; Medina,Cláudia S.; Fernandes,Maria J.B.; Simoni,Isabela C..
The aqueous extract from Polymnia sonchifolia leaves (AE) was tested for inhibitory activity on aflatoxin B1(AFB1) production and growth of Aspergillus flavus. The cytotoxicity of AE on Vero cells was also performed. Suspensions of A. flavus spores were inoculated into 50 mL of YES medium together with different concentrations of the AE. The aflatoxin B1 was extracted, analyzed by thin layer chromatography and quantified by photodensitometry. All the concentrations of AE induced inhibition of AFB1 production. The aqueous extract showed in vitro cytotoxicity to Vero cells only at concentrations above 500 µg/mL.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin B1; Aspergillus flavus; Polymnia sonchifolia; Cytotoxicity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200011
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Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus BJM
Flatschart,Roberto B.; Resende,Maurício.
Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
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Airborne fungi in the region of Cubatão, São Paulo State, Brazil BJM
Schoenlein-Crusius,Iracema H.; Trufem,Sandra F.B.; Grandi,Rosely A.P.; Milanez,Adauto I.; Pires-Zottarelli,Carmen L.A..
From March/1993 to March/1995, airborne fungi were isolated from the "Vale do Rio Moji" (highly affected by the air pollution caused by fertilizer industries, steel works, cement factories and chemical products industries, among others) and from the "Vale do Rio Pilões" (less affected by the air pollution), both in the municipality of Cubatão, São Paulo State, Brazil, by exposing Petri dishes with potato dextrose agar medium, placed one meter from the ground, during 5 min. After 5 days of incubation at 20ºC, the colonies of the fungi were purified and identified, resulting in the identification of 39 taxa, three unidentified strains of Fusarium and non-sporulating cultures. A total of 28 taxa, one unidentified strain of Fusarium and non-sporulating fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal diversity; Airborne fungi; Atlantic rainforest; Air pollution.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100014
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ANALYSES OF GENETIC VARIABILITY IN LENTINULA EDODES THROUGH MYCELIA RESPONSES TO DIFFERENT ABIOTIC CONDITIONS AND RAPD MOLECULAR MARKERS BJM
Maki,Cristina Sayuri; Teixeira,Flavia França; Paiva,Edilson; Paccola-Meirelles,Luzia Doretto.
The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Edible mushroom; RAPD; Mycelial growth.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300002
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Antagonism of Trichoderma species on Cladosporium herbarum and their enzimatic characterization BJM
Barbosa,Maria Angélica G.; Rehn,Kurt Georg; Menezes,Maria; Mariano,Rosa de Lima R..
The verrucose caused by Cladosporium herbarum reduces production and quality of Passion fruit (Passiflora edulis Sims.), a largely consumed tropical fruit. This work aimed to investigate the antagonism of Trichoderma species (T. polysporum, T. koningii, T. viride and T. harzianum) against Cladosporium herbarum, and to study the production of extracellular hydrolytic enzymes by the pathogen and the antagonists. The results showed considerable antagonistic potential for the biocontrol of C. herbarum isolates by all Trichoderma species, except T. koningii. The most distinguished effect was observed for T. polysporum. In relation to the pattern of esterase obtained by electrophoresis in poliacrylamyde gel, the major activity presented by the isolates was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passion fruit; Biological control; Extracellular enzymes; Fungi.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200005
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ANTAGONISTIC EFFECT OF EDIBLE MUSHROOM EXTRACT ON CANDIDA ALBICANS GROWTH BJM
Paccola,Edneia A. de Souza; Maki,Cristina Sayuri; Nobrega,Gisele M.A. de; Paccola-Meirelles,Luzia Doretto.
Five species of edible mushrooms, Lentinula edodes, Pleurotus ostreatus, Pholiota nameko, Macrolepiota bonaerensis and Agaricus blazei, were tested for their potential to inhibit the in vitro growth of the pathogenic yeast Candida albicans. Only L. edodes had a fungistatic effect on this human pathogen. The inhibitory compound was produced intra and extracellularly in submersed L. edodes culture, and was also present in fresh and dehydrated mushroom basidiocarps. The fungistatic compound was heat sensitive and lost activity after 72 hours.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mushrooms; Antibiotics; Candidiasis; Fungistatic effect.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300003
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ANTIBACTERIAL ACTIVITY OF LENTINULA EDODES GROWN IN LIQUID MEDIUM BJM
Ishikawa,Noemia Kazue; Kasuya,Maria Catarina Megumi; Vanetti,Maria Cristina Dantas.
The antibacterial activity of 35 isolates of Lentinula edodes, a shiitake mushroom, against Bacillus subtilis was evaluated by diffusion technique in agar with a semi-solid overlay. All isolates inhibited B. subtilis and the isolate Le1 promoted the formation of the largest inhibition zone. L. edodes Le1 also presented antibacterial activity against foodborne pathogens and food contaminant bacteria, particularly Gram-positive species. The antibacterial activity of the culture filtrate after 18-25 days of cultivation of L. edodes in broth at 25ºC was high. The inhibitory activity was observed only in the organic layer when the culture filtrate was partitioned between ethyl acetate and water, suggesting that the inhibitory substances have low polarity. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial activity; Lentinula edodes; Shiitake.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300008
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Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria BJM
Nascimento,Gislene G. F.; Locatelli,Juliana; Freitas,Paulo C.; Silva,Giuliana L..
The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. In addition, the possible synergistic effects when associated with antibiotics were studied. Extracts from the following plants were utilized: Achillea millifolium (yarrow), Caryophyllus aromaticus (clove), Melissa offficinalis (lemon-balm), Ocimun basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis (rosemary), Salvia officinalis (sage), Syzygyum joabolanum (jambolan) and Thymus vulgaris (thyme). The phytochemicals benzoic acid, cinnamic acid, eugenol and farnesol were also utilized. The highest antimicrobial potentials were observed for the extracts of Caryophyllus aromaticus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant extracts activity; Medicinal plants; Antimicrobial activity.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400003
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Antifungal properties of clove oil (Eugenia caryophylata) in sugar solution BJM
Núñez,Lidia; D'Aquino,Miguel; Chirife,Jorge.
The effect of temperature, concentration and contact time on the fungicidal effect of clove oleoresin dispersed in a concentrated sugar solution at 21 and 37ºC, and clove oleoresin at 0.2 to 0.8% (v/v) was studied. The test microorganisms were Candida albicans, Penicillium citrinum, Aspergillus niger and Trichophyton mentagrophytes. The fungicidal effect was enhanced at 37ºC; at this temperature short contact times (e.g. 1 min.) were enough to eliminate a microbial inoculum of 10(6) c.f.u./ml of C. albicans. Although clove oleoresin caused important lethal effect, P. citrinum and A. niger were more resistant. After 60 minutes, clove oleoresin dispersed (0.4% v/v) in concentrated sugar solution caused a 99.6% reduction of the initial population (10(6)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clove oil; Fungicide; Disinfectant; Sugar.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200010
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Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products BJM
Martinis,Elaine C.P. De; Públio,Márcia R.P.; Santarosa,Priscila R.; Freitas,Flávia Z..
Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Meat; Bacteriocins; Biopreservation; Listeria monocytogenes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100008
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Beijerinckia derxii stimulates the viability of non-N2-fixing bacteria in nitrogen-free media BJM
Barbosa,Heloiza R.; Thuler,Daniela Strauss; Shirakawa,Márcia Aiko; Miyasaka,Natália R. S..
The interactions between the nitrogen-fixing microorganism Beijerinckia derxii with two non-diazotrophic bacteria, either Escherichia coli or a facultative sulphur-oxidizing chemolitotroph, were studied in mixed cultures. Direct and indirect contact between B. derxii and E. coli were tested. B. derxii increased CFU numbers and/or maintained the viability of the non-diazotrophic bacteria, but neither growth nor nitrogenase activity of the nitrogen-fixing bacterium were affected by either partner.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beijerinckia derxii; Coculture; Viability.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300004
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Bioaccumulation of copper, zinc, cadmium and lead by Bacillus sp., Bacillus cereus, Bacillus sphaericus and Bacillus subtilis BJM
Costa,Antonio Carlos Augusto da; Duta,Flavia Pereira.
This work presents some results on the use of microbes from the genus Bacillus for uptake of cadmium, zinc, copper and lead ions. Maximum copper bioaccumulations were 5.6 mol/g biomass for B. sphaericus, 5.9 mol/g biomass for B. cereus and B. subtilis, and 6.4 mol/g biomass for Bacillus sp. Maximum zinc bioaccumulations were 4.3 mol/g biomass for B. sphaericus, 4.6 mol/g biomass for B. cereus, 4.8 mol/g biomass for Bacillus sp. and 5.0 mol/g biomass for B. subtilis. Maximum cadmium bioaccumulations were 8.0 mol/g biomass for B. cereus, 9.5 mol/g biomass for B. subtilis, 10.8 mol/g biomass for Bacillus sp. and 11.8 mol/g biomass for B. sphaericus. Maximum lead biomaccumulations were 0.7 mol/g biomass for B. sphaericus, 1.1 mol/g biomass for B. cereus, 1.4...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioaccumulation; Bacillus; Heavy metals.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100001
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Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms BJM
Silva,Wladimir Padilha da; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M..
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Atypical S.aureus; Coagulase; Thermonuclease; API-Staph; Baird-Parker agar; Milk; Mastitis; Environmental samples.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200008
Registros recuperados: 119
Primeira ... 123456 ... Última
 

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