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Registros recuperados: 4
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16S rRNA gene-based molecular analysis of mat-forming and accompanying bacteria covering organically-enriched marine sediments underlying a salmon farm in Southern Chile (Calbuco Island) Gayana
Aranda,Carlos; Paredes,Javier; Valenzuela,Cristian; Lam,Phyllis; Guillou,Laure.
The mat forming bacteria covering organic matter-enriched and anoxic marine sediments underlying a salmon farm in Southern Chile, were examined using 16S rRNA gene phylogenies. This mat was absent in the sea bed outside the direct influence of the farm (360 m outside fish cages). Based on nearly complete 16S rRNA gene sequences (-1500 bp), mat-forming filamentous cells were settled as the sulphur-oxidizing and putatively dissimilative nitrate-reducing Beggiatoa spp., being closely related (up to 97% sequence identity) to Beggiatoa spp. identified in eutrophic shallow sediments in northern Europe (Danish Limfjorden and German Dangast inlets). Their phylogenetic affiliation was consistent with their morphology as vacuolated and sulphur-containing cells...
Tipo: Journal article Palavras-chave: 16S rRNA genes; Beggiatoa mat; Salmon farming sediments; Sulphur cycle.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382010000200006
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Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte ArchiMer
Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf
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Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor ArchiMer
Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne.
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences...
Tipo: Text Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf
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Taxonomic assessment and antimicrobial screening of isolated bacteria from marine and terrestrial Antarctic samples. Repositório Alice
SILVA, T. R.; DUARTE, A. W. F.; PASSARINI, M. R. Z.; RUIZ, A. L. T. G.; FRANCO, C. H.; MORAES, C. B.; FANTINATTI-GARBOGGINI, F.; MELO, I. S. de; RODRIGUES, R. A.; OLIVEIRA, V. M..
Microorganisms dominate most of Antarctic ecosystem and play a crucial role in its functioning. They are called extremophilic microorganisms with unique and versatile metabolic properties with possible biotechnological applications in several areas. The aim of the present study was to taxonomically characterize psychrophilic microorganisms from Antarctic continent samples and to screen them for antimicrobial effects. Phylogenetic analysis revealed that most isolates shared 98?100 % sequence similarity to recognized species, including those recovered previously from the Antarctica environment, which belong to the major phyla Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria (classes Alpha and Gammaproteobacteria). A total of 361 bacteria strains,...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Antimicrobial activity; Antarctica; Antiproliferative effect; 16S rRNA genes; Bacterial taxonomy.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103537
Registros recuperados: 4
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