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Registros recuperados: 14
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Analysis of CATMA transcriptome data identifies hundreds of novel functional genes and improves gene models in the Arabidopsis genome Inra
Aubourg, S.; Martin-Magniette, M.L.; Brunaud, V.; Taconnat, L.; Bitton, F.; Balzergue, S.; Jullien, P.E.; Ingouff, M.; Thareau, V.; Schiex, T.; Lecharny, A.; Renou, J.P..
Background: Since the finishing of the sequencing of the Arabidopsis thaliana genome, the Arabidopsis community and the annotator centers have been working on the improvement of gene annotation at the structural and functional levels. In this context, we have used the large CATMA resource on the Arabidopsis transcriptome to search for genes missed by different annotation processes. Probes on the CATMA microarrays are specific gene sequence tags (GSTs) based on the CDS models predicted by the Eugene software. Among the 24 576 CATMA v2 GSTs, 677 are in regions considered as intergenic by the TAIR annotation. We analyzed the cognate transcriptome data in the CATMA resource and carried out data-mining to characterize novel genes and improve gene models....
Tipo: Journal Article Palavras-chave: ADNC; EXPRESSION DES GENES; SEQUENCE NUCLEOTIDIQUE; ARABIDOPSIS THALIANA; GENOME; MODELE; METHODE STATISTIQUE; PROTEINE; TRANSCRIPTION; ARN MESSAGER; PCR; RT-PCR CDNA MICROARRAY DATA; EXPRESSION PROFILES; SEQUENCE TAGS; ANNOTATION; THALIANA; NORMALIZATION; REGIONS; TOOLS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20116115e9a4&uri=/notices/prodinra1/2011/03/
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CATdb: a public access to Arabidopsis transcriptome data from the URGV-CATMA platform Inra
Gagnot, S.; Tamby, J.P.; Martin-Magniette, M.L.; Bitton, F.; Taconnat, L.; Balzergue, S.; Aubourg, S.; Renou, J.P.; Lecharny, A.; Brunaud, V..
CATdb is a free resource available at http://urgv.evry.inra.fr/CATdb that provides public access to a large collection of transcriptome data for Arabidopsis thaliana produced by a single Complete Arabidopsis Transcriptome Micro Array (CATMA) platform. CATMA probes consist of gene-specific sequence tags (GSTs) of 150500 bp. The v2 version of CATMA contains 24 576 GST probes representing most of the predicted A. thaliana genes, and 615 probes tiling the chloroplastic and mitochondrial genomes. Data in CATdb are entirely processed with the same standardized protocol, from microarray printing to data analyses. CATdb contains the results of 53 projects including 1724 hybridized samples distributed between 13 different organs, 49 different developmental...
Tipo: Journal Article Palavras-chave: CDNA MICROARRAY DATA; SEQUENCE TAGS; INFORMATION; GENOME; NORMALIZATION; BIOLOGY; GENE COMPONENTS ; ADNC; SEQUENCE NUCLEOTIDIQUE; NORMALISATION; BIOLOGIE; ARABIDOPSIS THALIANA; MUTATION; MITOCHONDRIE; CHLOROPLASTE; METHODE STATISTIQUE; EXPRESSION DES GENES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20111a416d88&uri=/notices/prodinra1/2011/03/
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Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant Inra
Durand, N.; Carot-Sans, G.; Chertemps, T.; Bozzolan, F.; Party, V.; Renou, M.; Debernard, S.; Rosell, G.; Maïbèche-Coisne, M..
Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/ pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In...
Tipo: Journal Article Palavras-chave: LEPIDOPTERA; NOCTUIDAE; HEXAPODA; PCR; ADNC; SDS PAGE; EXPRESSION DES GENES; ELECTROPHORESE; CARBOXYLESTERASE; SPODOPTERA LITTORALIS; OLFACTION; ODEEUR; PLANTE HOTE; INSECTE; ENZYME; BIOLOGIE MOLECULAIRE; BIOCHIMIE; ELECTROPHYSIOLOGIE; PHEROMONE; PHEROMONE SEXUELLE; RECOMBINAISON; PROTEINE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010229c7cd1&uri=/notices/prodinra1/2011/05/
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Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae Inra
Brigaud, I.; Grosmaître, X.; Francois, M.C.; Jacquin-Joly, E..
In insects, biogenic amines have been shown to play an important role in olfactory plasticity. In a first attempt to decipher the underlying molecular mechanisms, we report the molecular cloning and precise expression pattern of a newly identified octopamine/tyramine-receptor-encoding gene in the antennae of the noctuid moth Mamestra brassicae (MbraOAR/TAR). A full-length cDNA has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction; the deduced protein exhibits high identities with previously identified octopamine/tyramine receptors in other moths. In situ hybridization within the antennae has revealed that MbraOAR/TAR is expressed at the bases of both pheromone-sensitive and non-sensitive...
Tipo: Journal Article Palavras-chave: CLONAGE; LOBE ANTENNAIRE; NOCTUIDAE; MITE; HEXAPODA; ARTHROPODA; AMINE BIOGENIQUE; RECEPTEUR; CLONAGE MOLECULAIRE; ADNC; POLYMORPHISME; PCR; PHEROMONE; LEPIDOPTERA; MAMESTRA BRASSICAE OCTOPAMINE/TYRAMINE RECEPTOR; EXPRESSION PATTERN; OLFACTION; OLFACTORY PLASTICITY.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201084fc8d7c&uri=/notices/prodinra1/2010/11/
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Cloning of a cDNA encoded by a member of the Arabidopsis thaliana ATP sulfurylase multigene family. Expression studies in yeast and in relation to plant sulfur nutrition Inra
Logan, H.M.; Cathala, N.; Grignon, C.; Davidian, J.C..
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; ATP; SULFURYLASE; CLONAGE MOLECULAIRE; ADNC; SEQUENCE NUCLEOTIDIQUE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGUE; ACTIVITE ENZYMATIQUE; TRANSCRIPTION; SULFATE; BRASSICA NAPUS.
Ano: 1996 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200029830090142&uri=/notices/prodinra1/2010/09/
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Cloning of an octopamine/tyramine receptor and plasticity of its expression as a function of adult sexual maturation in the male moth Agrotis ipsilon Inra
Duportets, L.; Barrozo, R.B.; Bozzolan, F.; Gaertner, C.; Anton, S.; Gadenne, C.; Debernard, S..
In the male moth Agrotis ipsilon behavioural response and antennal lobe (AL) neuron sensitivity to the female-produced sex pheromone increase with age and juvenile hormone (JH) level. We recently showed that the neuromodulator, octopamine (OA), interacts with JH in this age-dependent olfactory plasticity. To further elucidate its role, we cloned a full cDNA encoding a protein that presents biochemical features essential to OA/tyramine receptor (AipsOAR/TAR) function. The AipsOAR/TAR transcript was detected predominantly in the antennae, the brain and, more specifically, in ALs where its expression level varied concomitantly with age. This expression plasticity indicates that AipsOAR/TAR might be involved in central processing of the pheromone signal during...
Tipo: Journal Article Palavras-chave: LOBE ANTENNAIRE; LEPIDOPTERA; NOCTUIDAE; AGROPIS IPSILON; RECEPTEUR; OLFACTION; MITE; INSECTE; MATURATION SEXUELLE; ARTHROPODA; HEXAPODA; NEURONE; PHEROMONE; HORMONE; ADNC; CLONAGE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL ANTENNAL LOBE; OCTOPAMINE/TYRAMINE RECEPTOR; OLFACTORY PROCESSING; MATURATION; EXPRESSION PLASTICITY.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102d18e75d&uri=/notices/prodinra1/2011/02/
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Construction of full-length cDNA clones of lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants Inra
Yang, S.J.; Revers, F.; Souche, S.; Lot, H.; Le Gall, O.; Candresse, T.; Dunez, J..
 A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL cDNA in order to relieve possible deleterious effects of viral sequences to Escherichia coli cells and to evaluate the effects of the presence of the intron on the FL cDNA infectivity. The intron-less FL cDNA was found to be as stable as its intron-containing counterpart in E. coli. Sequence analysis of progeny RNA derived from plants inoculated with the intron-containing FL cDNA demonstrated that the inserted intron was perfectly spliced out....
Tipo: Journal Article Palavras-chave: LMV; LETTUCE MOSAIC VIRUS; VIRUS DE LA MOSAIQUE DE LA LAITUE; ADNC; VIRUS DES VEGETAUX; MALADIE DES PLANTES; CLONE.
Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100011156086856&uri=/notices/prodinra1/2010/11/
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Functional annotation, genome organization and phylogeny of the grapevine (Vitis vinifera) terpene synthase gene family based on genome assembly, FLcDNA cloning, and enzyme assays Inra
Martin, D.M.; Aubourg, S.; Schouwey, M.B.; Daviet, L.; Schalk, M.; Toub, O.; Lund, S.T.; Bohlmann, J..
Background: Terpenoids are among the most important constituents of grape flavour and wine bouquet, and serve as useful metabolite markers in viticulture and enology. Based on the initial 8-fold sequencing of a nearly homozygous Pinot noir inbred line, 89 putative terpenoid synthase genes (VvTPS) were predicted by in silico analysis of the grapevine (Vitis vinifera) genome assembly [1]. The finding of this very large VvTPS family, combined with the importance of terpenoid metabolism for the organoleptic properties of grapevine berries and finished wines, prompted a detailed examination of this gene family at the genomic level as well as an investigation into VvTPS biochemical functions.Results: We present findings from the analysis of the up-dated 12-fold...
Tipo: Journal Article Palavras-chave: CHROMATOGRAPHIE; SPECTROMETRIE DE MASSE; CLONAGE; ADNC; METABOLISME SECONDAIRE; PHYLOGENIE; ENZYME; TERPENOIDE; FLAVEUR; ODEUR; CARTOGRAPHIE; PROPRIETE ORGANOLEPTIQUE ENT-COPALYL DIPHOSPHATE; CHROMATOGRAPHY-MASS SPECTROMETRY; SESQUITERPENE SYNTHASES (-)-GERMACRENE-D SYNTHASE SECONDARY METABOLISM ARABIDOPSIS-THALIANA; LIMONENE SYNTHASE ESCHERICHIA-COLI; RESISTANCE GENES; CDNA CLONING.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011fadf0898&uri=/notices/prodinra1/2011/01/
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Identification of differentially expressed genes in the hydrothermal vent shrimp Rimicaris exoculata exposed to heat stress Inra
Cottin, D.; Shillito, B.; Chertemps, T.; Tanguy, A.; Léger, N.; Ravaux, J..
The deep-sea vent shrimp Rimicaris exoculata dominates the vagile megafauna at most vent sites along the Mid-Atlantic Ridge. This shrimp swarms around the hot end of the hydrothermal biotope where temperature can exceed its critical maximal temperature (33–38.5 ± 2 °C). It may therefore be subjected to a thermal regime that is assumed to be stressful for animals. In this study, we used a global transcriptomic approach by constructing suppression subtractive hybridization cDNA libraries in order to identify specific up- and down-regulated genes in R. exoculata exposed to a severe heat stress (1 h at 30 °C). A total of 218 sequences representing potentially highly expressed genes in thermally stressed shrimp were obtained. Expression of 11 genes involved in...
Tipo: Journal Article Palavras-chave: ARTHROPODA; CRUSTACEA; MALACOSTRACA; ALVINOCARIDIDAE; EXPRESSION DES GENES; STRESS THERMIQUE; EFFET DE LA TEMPERATURE; CLONAGE MOLECULAIRE; ADNC; HYBRIDATION; REGULATION; TRANSCRIPTION; PROTEINE RIMICARIS EXOCULATA; HYDROTHERMAL VENT; HEAT STRESS; SUPPRESSION SUBTRACTIVE HYBRIDIZATION; REAL-TIME PCR.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010af1480fe&uri=/notices/prodinra1/2010/11/
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Isolation of two Arabidopsis cDNAs involved in early steps of molybdenum cofactor biosynthesis by functional complementation of Escherichia coli mutants Inra
Hoff, T.; Schnorr, K.M.; Meyer, C.; Caboche, M..
Tipo: Journal Article Palavras-chave: ADNC; MOLYBDENE; COFACTEUR; CARTE CHROMOSOMIQUE; CLONAGE DE GENE.
Ano: 1995 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9600002046054015&uri=/notices/prodinra1/2010/09/
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Molecular basis of female-specific odorant responses in Bombyx mori Inra
Anderson, A.R.; Wanner, K.W.; Trowell, S.C.; Warr, C.G.; Jacquin-Joly, E.; Zagatti, P.; Robertson, H.; Newcomb, R.D..
Males and females of many moth species exhibit important differences in sexual behaviours. Much research in this field has focused on the male-specific behaviour, electrophysiology and molecular biology of sex pheromone reception. Female-specific behaviours have been less well studied although, like male-specific behaviours, they could provide opportunities for intervention and management of lepidopteran pests. Previously, we identified genes encoding putative odorant receptors (ORs) from the genome of the silkworm, Bombyx mori, some of which have higher levels of steady-state transcript in the antennae of adult females compared with males. We have identified the full-length cDNA sequences of some of these ORs and described a novel OR that is part of a...
Tipo: Journal Article Palavras-chave: TERPENE; ACIDE BENZOIQUE; PHENYLETHANOL; BENZALDEHYDE; ELECTROPHYSIOLOGIE; SEQUENCE NUCLEOTIDIQUE; ODEUR; HYPOTHENEMUS HAMPEI; ARTHROPODA; HEXAPODA; INSECTE; RECEPTEUR; PLANTE; COMPOSE VOLATILE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL; ADNC; BIOLOGIE MOLECULAIRE; LEPIDOPTERA BOMBYX MORI; OLFACTION; ODORANT RECEPTORS; PLANT VOLATILES; PHEROMONE.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101ffb559b&uri=/notices/prodinra1/2010/11/
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Molecular characterization of a phospholipase C β potentially involved in moth olfactory transduction Inra
Chouquet, B.; Lucas, P.; Bozzolan, F.; Solvar, M.; Maïbèche-Coisné, M.; Durand, N.; Debernard, S..
To clarify the role of phospholipase C (PLC) in insect olfactory transduction, we have undertaken its molecular identification in the moth Spodoptera littoralis. From the analysis of a male antennal expressed sequence tag library, we succeeded in cloning a full-length cDNA encoding a PLC that belongs to the cluster of PLC-b subtypes. In adult males, the PLC-b transcript was located predominantly in brain and antennae where its presence was detected in the olfactory sensilla trichodea. Moreover, PLC-b was expressed in antennae at the beginning of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. Taken together, these results provided molecular evidence for the putative participation of...
Tipo: Journal Article Palavras-chave: INSECTE; PHOSPHOLIPASE; OLFACTION; ODEUR; MITE; TECHNIQUE ANALYTIQUE; SEQUENCE NUCLEOTIDIQUE; EXPRESSION DES GENES; ADNC; TRANSCRIPTION; NORTHERN BLOT; ELECTROPHORESE; PCR; CLONAGE MOLECULAIRE; ANALYSE PHYLOGENETIQUE; LEPIDOPTERA; NOCTUELLE MEDITERRANEENNE EXPRESSION PATTERNS; OLFACTORY SYSTEM; PHOSPHOLIPASE C; SPODOPTERA LITTORALIS; SIGNAL TRANSDUCTION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20108d7c7c75&uri=/notices/prodinra1/2010/09/
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Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis-cassava interaction Inra
Verdier, V.; Restrepo, S.; Mosquera, G.; Jorge, V.; Lopez, C..
Cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis (Xam), is a widespread disease that affects cassava (Manihot esculenta Crantz). Studies on the pathogen population structure, pathogen diagnosis, identification and expression of plant genes involved in resistance have been carried out. Different molecular techniques were developed to assess the genetic diversity among the Xampopulations. Characterization of Xam population dynamics over time had enable us to determine the different factors that are associated with resistance breakdown and those that influence the genetic structure or virulence phenotypes of the pathogen’s population. Methods for detecting the pathogen in vegetative planting materials and true seeds were developed and...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; EXPRESSION DES GENES; BACTERIOSE; ADNC; GENE CANDIDAT; DETECTION DE QTL.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073f223d29&uri=/notices/prodinra1/2007/04/
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The 20-hydroxyecdysone-induced signalling pathway in G2/M arrest of Plodia interpunctella imaginal wing cells Inra
Siaussat, D.; Bozzolan, F.; Porcheron, P.; Debernard, S..
The mechanisms involved in the control of cellular proliferation by the steroid hormone 20-hydroxyecdysone (20E) in insects are not known. We dissected the 20E signalling pathway responsible for G2/M arrest of imaginal cells from the IAL-PID2 cells of the Indian meal moth Plodia interpunctella. We first used a 5′–3′ RACE-based strategy to clone a 4479 bp cDNA encoding a putative P. interpunctella HR3 transcription factor named PiHR3. The deduced amino acid sequence of PiHR3 was highly similar to those of HR3 proteins from other lepidopterans, e.g. Manduca sexta and Bombyx mori. Using double-stranded RNA-mediated interference (dsRNAi), we then succeeded in blocking the ability of 20E to induce the expression of PiEcR-B1, PiUSP-2 and PiHR3 genes that encode...
Tipo: Journal Article Palavras-chave: PLODIA INTERPUNCTELLA; EUCARYOTE; ARTHROPODA; HEXAPODA; INSECTE; LEPIDOPTERA; HORMONE STEROIDE; MITE; CLONAGE; ADNC; EXPRESSION DES GENES; TRANSCRIPTION; SEQUENCE NUCLEOTIDIQUE; ACIDE AMINE  RNA INTERFERENCE; STEROID HORMONE RECEPTOR SUPERFAMILY; G2/M ARREST; B CYCLIN; SIGNALLING PATHWAY; IMAGINAL CELLS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103c688c9f&uri=/notices/prodinra1/2010/10/
Registros recuperados: 14
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