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| Teyssier, C.; Grondin, C.; Bonhomme, L.; Lomenech, A.M.; Vallance, M.; Morabito, D.; Label, P.; Lelu-Walter, M.A.. |
| An integrated physiological and proteomic approach was used to investigate the effects of high gellan gum concentration in the medium during maturation of somatic embryos of hybrid larch, by comparing embryos incubated in media with a high gellan gum concentration (8 gL-1) and the conventional concentration (4 gL-1) after 1, 3, 6 and 8 weeks of maturation. Due to the reduced availability of water in the 8 gL-1 medium, the embryos cultivated on it had lower osmotic potential and water contents, but higher dry weights, at 8 weeks than embryos cultivated on the standard medium. The high gellan gum concentration induced a desiccation as occurring during zygotic embryo maturation. Total soluble proteins were extracted from embryos with TCA-acetone after 1 and 8... |
| Tipo: Journal Article |
Palavras-chave: MELEZE HYBRIDE; EMBRYOGENESE SOMATIQUE; ANALYSE PROTEOMIQUE; AGENT GELIFIANT; TENEUR EN EAU; POTENTIEL OSMOTIQUE; MATURATION DE L'EMBRYON SOMATIQUE HYBRID LARCH; SOMATIC EMBRYOGENESIS; PROTEOMIC ANALYSIS; GELLING AGENT; WATER CONTENT; OSMOTIC POTENTIAL; SOMATIC EMBRYO MATURATION. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20118c1c4285&uri=/notices/prodinra1/2011/05/ |
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