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Analysis of the genetic diversity of Eucalyptus cladocalyx (sugar gum) using ISSR markers Agronomy
Ballesta, Paulina; Mora, Freddy; Contreras-Soto, Rodrigo Ivan; Ruiz, Eduardo; Perret, Sandra.
Eucalyptus cladocalyx F. Muell is a tree endemic to southern Australia and is distributed across four isolated regions: Kangaroo Island, southern Flinders Ranges, and two geographical zones in Eyre Peninsula. E. cladocalyx is capable of growing under extreme environmental conditions, including dry and saline soils. The objective of this study was to analyze genetic diversity in 45 half-sib families planted in northern Chile that are distributed across five different zones (provenances). Genetic variability was assessed using ISSR (Inter Simple Sequence Repeat) molecular markers. The results showed low levels of genetic diversity within populations (He = 0.113 to 0.268) in contrast with other Eucalyptus species. In addition, there was a significant genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetica; Florestal DNA pooling; Half-sib families; AMOVA; Clustering analysis.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19307
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Assessment of est-ssr markers for genetic analysis on coffee. Repositório Alice
MISSIO, R. F.; CAIXETA, E. T.; ZAMBOIM, E. M.; PENA, G. F.; RIBEIRO, A. P.; ZAMBOLIM, L.; PEREIRA, A. A.; SAKIYAMA, N. S..
2009
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: DNA markers; Coffea sp; UPGMA; AMOVA; Microsatellite markers.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/880408
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Comparative genetic diversity and genetic structure of three chinese silkworm species Bombyx mori L. (Lepidoptera: Bombycidae), Antheraea pernyi Guérin-Meneville and Samia cynthia ricini donovan (Lepidoptera: Saturniidae) Neotropical Entomology
Liu,Yan-Qun; Qin,Li; li,Yu-Ping; Wang,Huan; Xia,Run-Xi; Qi,Yong-Hong; li,Xi-Sheng; Lu,Cheng; Xiang,Zhong-Huai.
The genetic diversity and genetic structure of three Chinese silkworm species Bombyx mori L., Antheraea pernyi Guérin-Meneville and Samia cynthia ricini Donovan were comparatively assessed based on RAPD markers. At the species level, A. pernyi and B. mori showed high levels of genetic diversity, whereas S. cynthia ricini showed low level of genetic diversity. However, at the strain level, A. pernyi had relatively highest genetic diversity and B. mori had lowest genetic diversity. Analysis of molecular variance (AMOVA) suggested that 60% and 72% of genetic variation resided within strains in A. pernyi and S. cynthia ricini, respectively, whereas only 16% of genetic variation occurred within strains in B. mori. In UPGMA dendrogram, individuals of A. pernyi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; Genetic variation; AMOVA; Dendrogram.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000600019
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Estrutura genética populacional de bacurizeiros na região Meio-Norte do Brasil. Repositório Alice
SOUZA, I G. de B.; LIMA, P. S. da C..
A região Meio-Norte caracteriza-se pela sua biodiversidade, com destaque para espécies frutíferas nativas, como, por exemplo, o bacurizeiro, cujos frutos são apreciados na alimentação pelas populações locais. A fragmentação e/ou desmatamento que ocorre nessa área causa danos para populações de plantas, afetando a estrutura genética destas. Assim esse estudo verificou a estruturação genética em cinco populações de bacurizeiro dos Estados do Maranhão e Piauí por meio de marcadores entre simples sequências repetidas (ISSR) e da análise de variância molecular. Da diversidade genética total, 28,88% foi dividido entre as populações e 77,16% dentro das populações, portanto há estruturação significativa entre as populações.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: AMOVA; Bacuri; Fruta nativa.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/952958
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Genetic characterization of Japanese plum cultivars (Prunus salicina) using SSR and ISSR molecular markers Ciencia e Investigación Agraria
Carrasco,Basilio; Díaz,Carole; Moya,Mario; Gebauer,Marlene; García-González,Rolando.
The genetic characterization of 29 elite Japanese plum cultivars (Prunus salicina) and 4 Prunus cultivars was carried out by analyzing 97 Simple Sequence Repeat (SSR) alleles and 232 binary Inter Simple Sequence Repeat (ISSR) loci. A high level of genetic variability was found for these two molecular markers among the Japanese plum cultivars compared to other Prunus species. On average, the variability found by analyzing the SSR alleles were Na = 12.1, Ne = 5.2, Ho = 0.9, He = 0.8 and F= -0.127, whereas ISSR yielded values of h = 0.15 and I = 0.27. The genetic relationship among cultivars was estimated with Principal Coordinate Analysis (PCA) and a Bayesian clustering approach using the software program Structure. This program identified two subgroups...
Tipo: Journal article Palavras-chave: AMOVA; Bayesian analysis; Genetic distance; PCA; Plum; Structure.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300012
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Genetic diversity in two variants of Orobanche gracilis Sm. [var. gracilis and var. deludens (Beck) A. Pujadas] (Orobanchaceae) from different regions of Spain Electron. J. Biotechnol.
Román,Belén; Hernández,Rosabell; Pujadas-Salvá,Antonio José; Cubero,José Ignacio; Rubiales,Diego; Satovic,Zlatko.
The pattern of genetic variation among populations of two Orobanche gracilis Sm. taxa (var. gracilis and var. deludens (Beck) A. Pujadas) from Northern and Southern Spain growing on different hosts was analysed using RAPD markers. The diversity analysis within populations revealed a higher level of diversity in the populations from the North when compared to the Southern ones. The results of principal co-ordinate analysis (PCoA) based on Dice distances among samples clearly established the separation of samples according to the taxonomical variety and the geographical origin of each population. The Southern populations of both var. gracilis and var. deludens were more differentiated among them than those of var. gracilis from the North. The analysis of...
Tipo: Journal article Palavras-chave: AMOVA; Molecular markers; Orobanche gracilis; Parasitic plants; Population genetics.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200006
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Genetic diversity of Bemisia tabaci (Genn.) Populations in Brazil revealed by RAPD markers Genet. Mol. Biol.
Lima,L.H.C.; Campos,L.; Moretzsohn,M.C.; Návia,D.; Oliveira,M.R.V. de.
Bemisia tabaci (Genn.) was considered a secondary pest in Brazil until 1990, despite being an efficient geminivirus vector in beans and soybean. In 1991, a new biotype, known as B. tabaci B biotype (=B. argentifolii) was detected attacking weed plants and causing phytotoxic problems in Cucurbitaceae. Nowadays, B. tabaci is considered one of the most damaging whitefly pests in agricultural systems worldwide that transmits more than 60 different plant viruses. Little is known about the genetic variability of these populations in Brazil. Knowledge of the genetic variation within whitefly populations is necessary for their efficient control and management. The objectives of the present study were to use RAPD markers (1) to estimate the genetic diversity of B....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Whitefly; Molecular markers; UPGMA; AMOVA.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200016
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Genetic similarity of Jatropha curcas accessions based on AFLP markers. Repositório Alice
SANTOS, C. A. F.; DRUMOND, M. A.; RODRIGUES, M. A.; EVANGESLISTA, M. R. V..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Dendrograma; AMOVA; Variabilidade genética; AFLP; Jatropha curcas; Pinhão manso.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/874096
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Genetic variability of Pantaneiro horse using RAPD-PCR markers R. Bras. Zootec.
Egito,Andréa Alves do; Fuck,Beatriz Helena; McManus,Concepta; Paiva,Samuel Rezende; Albuquerque,Maria do Socorro Maués; Santos,Sandra Aparecida; Abreu,Urbano Gomes Pinto de; Silva,Joaquim Augusto da; Sereno,Fabiana Tavares Pires de Souza; Mariante,Arthur da Silva.
Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. The analysis of molecular variance (AMOVA) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). Analysis of the genetic variability between pairs of populations presented higher estimates for the five Pantaneiro populations with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AMOVA; Arabian; English Thoroughbred; Genetic diversity; Genetic resource; Mangalarga Marchador.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007000400007
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Genetic variability of Pantaneiro horse using RAPD-PCR markers UnB - FAB
Egito, Andréa Alves do; Fuck, Beatriz Helena; Pimentel, Concepta Margaret McManus; Paiva, Samuel Rezende; Albuquerque, Maria do Socorro Maués; Santos, Sandra Aparecida; Abreu, Urbano Gomes Pinto de; Silva, Joaquim Augusto da; Sereno, Fabiana Tavares Pires de Souza; Mariante, Arthur da Silva.
Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA – Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. The analysis of molecular variance (AMOVA) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). Analysis of the genetic variability between pairs of populations presented higher estimates for the five Pantaneiro populations with the...
Tipo: Article Palavras-chave: AMOVA; Equino; Diversidade genética; Mangalarga Marcador; Puro-Sangue Inglês; Variação (Biologia).
Ano: 2007 URL: http://hdl.handle.net/10482/6845
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Human population genetic structure detected by pain-related mu opioid receptor gene polymorphisms Genet. Mol. Biol.
López Soto,Eduardo Javier; Catanesi,Cecilia Inés.
Several single nucleotide polymorphisms (SNPs) in the Mu Opioid Receptor gene (OPRM1) have been identified and associated with a wide variety of clinical phenotypes related both to pain sensitivity and analgesic requirements. The A118G and other potentially functional OPRM1 SNPs show significant differences in their allele distributions among populations. However, they have not been properly addressed in a population genetic analysis. Population stratification could lead to erroneous conclusions when they are not taken into account in association studies. The aim of our study was to analyze OPRM1 SNP variability by comparing population samples of the International Hap Map database and to analyze a new population sample from the city of Corrientes,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: OPRM1; SNPs; A118G; AMOVA; Population genetics.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200152
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Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP BJM
Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V..
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673
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Morphological and genetic differentiation among Chilean populations of Bufo spinulosus (Anura: Bufonidae) RChHN
Méndez,Marco A.; Soto,Eduardo R.; Correa,Claudio; Veloso,Alberto; Vergara,Eliseo; Sallaberry,Michel; Iturra,Patricia.
Bufo spinulosus has a wide and fragmented range distribution in Chile (18° to 33° S) along altitudinal and latitudinal gradients. Genetic variation was estimated using RAPD (Random Amplified Polymorphic DNA) markers in 10 populations from northern and central Chile. Morphometric and genetic information was analyzed as a function of geographical origin. The correlation between genetic and morphometric differentiation was analyzed by the Mantel test. An increase in body size as a function of latitude was observed. Specimens from El Tatio had the smallest body size and the greatest morphometric divergence. The AMOVA applied to genetic data indicated that 57.85 % of the variance is explained by interregional differences and that 30.12 % of the...
Tipo: Journal article Palavras-chave: Amphibians; Geographic variation; Morphometry; RAPDs; AMOVA.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2004000300014
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Morphological variation and isozyme diversity in Dioscorea alata L. landraces from Vale do Ribeira, Brazil Scientia Agricola
Bressan,Eduardo de Andrade; Briner Neto,Thiago; Zucchi,Maria Imaculada; Rabello,Ronaldo José; Veasey,Elizabeth Ann.
Traditional growers of the Vale do Ribeira, São Paulo State, grow and make use of several D. alata landraces. This study assessed the genetic diversity of 16 landraces, using isozymatic and morphological markers and comparing them with 19 commercial varieties of D. alata. Their distribution in different levels of organization such as households and communities of the Vale do Ribeira was evaluated. Isozymatic analyses were performed with polyacrylamide (six systems) and starch gels (one system), while the morphological analyses were carried out with 24 traits. Due to the polyploid nature of this species, the isozymatic bands were scored as binary data. Morphological traits were also scored as binary data. Principal coordinates and cluster analyses were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AMOVA; Traditional agriculture; Genetic diversity; Water yam.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400016
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RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey Electron. J. Biotechnol.
Pinarkara,Emine; Kayis,Seyit A; Hakki,Erdogan E; Sag,Ayla.
Cannabis sativa L. is a multiple-use plant. However, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 29 different locations of Turkey, were used. Interests were to identify the genetic relatedness of the seized samples and to partition molecular variance between and within populations. Randomly Amplified Polymorphic DNAs were employed for analysis based on single plant material and bulked samples of them. Data were analysed via cluster and principal coordinate analyses (PCoA). Analysis of molecular variance (AMOVA) was performed to obtain variations between and within populations. Cannabis accessions...
Tipo: Journal article Palavras-chave: AMOVA; Cluster analysis; Individualization of seized marijuana; Principle coordinate analysis; Randomly amplified polymorphic DNA; UPGMA.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100005
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Similaridade genética entre acessos de goiabeiras e araçazeiros baseada em marcadores moleculares AFLP. Repositório Alice
CORRÊA, L. C.; SANTOS, C. A. F.; LIMA, G. P. P.; RODRIGUES, M. A.; COSTA, T. P. P..
2011
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Psidium; Banco Ativo de Germoplasma; Melhoramento vegetal; AMOVA; Diversidade genética; Marcador molecular; Goiaba.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/904734
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Similaridade genética entre acessos de goiabeiras e araçazeiros baseada em marcadores moleculares AFLP Rev. Bras. Frutic.
Corrêa,Luiz Claudio; Santos,Carlos Antonio Fernandes; Lima,Giuseppina Pace Pereira; Rodrigues,Marciene Amorim; Costa,Tuany Priscila Pereira.
Espécies do gênero Psidium, como goiabeira e araçazeiros, são economicamente importantes e têm como área de diversidade genética primária o Brasil. Foram determinadas as relações genéticas, com base no marcador AFLP, para acessos do Banco Ativo de Germoplasma (BAG) de Psidium da Embrapa Semiárido, para orientar trabalhos de melhoramento e de manejo de recursos genéticos do gênero. Foram analisados 88 acessos, sendo 64 de goiabeira e 24 de araçazeiros, coletados em dez Estados brasileiros, adotando-se para agrupamento o dendrograma UPGMA, considerando a matriz de similaridade do coeficiente de Jaccard de 149 bandas polimórficas de AFLP de 16 combinações dos iniciadores EcoRI e MseI. A análise da variância de dados moleculares foi realizada considerando a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psidium; Banco ativo de germoplasma; Melhoramento vegetal; AMOVA.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000300021
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Similaridade genética entre híbridos F1 de cajueiro identificada com a adoção de iniciadores RAPD. Repositório Alice
BORDALLO, P. do N.; HAWERROTH, M. C.; OLIVEIRA, L. C. P..
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Anacardium sp; Radom Amplified Polymorphic; DNA; Coeficiente de Jaccard; AMOVA; Anacardium sp; Radom Amplified Polymorphic; DNA; Coeficiente de Jaccard; AMOVA.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1029177
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Structure and genetic relationships between Brazilian naturalized and exotic purebred goat domestic goat (Capra hircus) breeds based on microsatellites Genet. Mol. Biol.
Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli.
The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010
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Variación genética de poblaciones de Brevipalpus phoenicis (Acari: Tenuipalpidae), vector del Citrus leprosis virus tipo citoplásmico. Colegio de Postgraduados
Sánchez Velázquez, Eber Josué.
Brevipalpus phoenicis es considerado un vector importante del Citrus leprosis virus C (CiLV-C) agente causal de la leprosis tipo citoplásmico, enfermedad que afecta cítricos de variedades dulces principalmente. En este estudio se obtuvieron muestras de Brevipalpus phoenicis en huertos de naranja dulce (Citrus sinensis) de ocho y 12 localidades de Brasil y México, respectivamente. La estructura poblacional, la variación genética, así como el análisis filogenético de las muestras recolectadas fueron estudiados a partir de secuencias de nucleótidos del gen citocromo oxidasa subunidad I (COI). Se observó la existencia de dos grupos morfológicos (“B” y “C”) en muestras de ambos países, siendo éste el primer reporte del grupo “C” en el territorio mexicano. La...
Palavras-chave: Morfoespecies; Red de haplotipos; AMOVA; Morphospecies; Haplotype network; Entomología y Acarología; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2222
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