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Registros recuperados: 18 | |
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Davis, E.L.; Allen, R.; Hussey, R.S.. |
La microscopie en immunofluorescence a été utilisée avec des anticorps monoclonaux pour montrer que la production d'antigènes dans les glandes oesophagiennes de #Meloidogyne incognita$ est régulée par le développement durant la phase parasite et que quelques-uns de ces antigènes peuvent être excrétés au travers du stylet. Plusieurs antigènes des glandes oesophagiennes sont temporairement exprimés dans les glandes subventrales et dorsale des juvéniles préparasites de deuxième stade (J2), des juvéniles parasites (J2) et des femelles adultes. Deux anticorps monoclonaux reconnaissant les glandes subventrales de J2 et un anticorps monoclonal reconnaissant la glande dorsale et les glandes subventrales de J2, reconnaissent également les sécrétions passant au... |
Tipo: Text |
Palavras-chave: NEMATODE PHYTOPARASITE; DEVELOPPEMENT BIOLOGIQUE; IMMUNOFLUORESCENCE; PROTEINE; ANTIGENE; MARQUAGE; ANTICORPS MONOCLONAL. |
Ano: 1994 |
URL: http://www.documentation.ird.fr/hor/fdi:39993 |
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Uhde, K.; Kerschbaumer, R.J.; Koenig, R.; Hirsch, S.; Lemaire, O.; Boonham, N.; Roake, W.; Himmler, G.. |
The detection of Beet necrotic yellow vein virus (BNYVV) instored sugar beets by means of monoclonal antibodies or antibody singlechain fragments (scFv) often poses problems, because the immunodominantC-terminal epitope of the viral coat protein is readily lost due toproteolysis. Clones which produce scFv specific for protease-stableBNYVV epitopes were selected from two naive phage display libraries.Fusion proteins of the scFv with a human IgG kappa chain (expressed fromthe newly designed vector pCL) or with alkaline phosphatase,respectively, allow the ELISA detection of BNYVV even in stored sugarbeets with a sensitivity which was comparable or often higher than thatachieved with polyclonal antibodies |
Tipo: Journal Article |
Palavras-chave: BNYVV; BEET NECROTIC YELLOW VEIN VIRUS; EPITOPE; COQUE VIRALE; VIRUS DES VEGETAUX; ANTICORPS MONOCLONAL; TEST ELISA. |
Ano: 2000 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100016386085053&uri=/notices/prodinra1/2010/11/ |
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Kohli, E.; Pothier, P.; Tosser, G.; Cohen, J.; Sandino, A.M.; Spencer, E.. |
Six monoclonal antibodies specific for the major capsid protein of rotavirus, VP6, previously characterized, were tested in a biological assay for their capacity to block the transcriptase activity associated with the single-shelled particles. The results showed that two MAbs (RV-50 and RV-133), specific for distinct antigenic sites, were able to block the transcription when they were incubated with a purified baculovirus-expressed group A VP6, prior to the reconstitution of the single-shelled particles from the cores, suggesting that at least two domains are involved in active single-shelled particle reconstitution. The results obtained previously from immunochemistry of synthetic peptides did not allow us to attribute this biological activity to a... |
Tipo: Journal Article |
Palavras-chave: ANTICORPS MONOCLONAL; TRANSCRIPTION. |
Ano: 1994 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500004507051834&uri=/notices/prodinra1/2010/11/ |
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Valenzuela-Fernandez, A.; Palanche, T.; Amara, A.; Magerus, A.; Altmeyer, R.; Delaunay, T.; Virelizier, J.L.; Baleux, F.; Galzi, J.L.; Arenzana-Seisdedos, F.. |
The chemokine stromal cell-derived factor 1 (SDF-1) is the natural ligand for CXC chemokine receptor 4 (CXCR4). SDF-1 inhibits infection of CD4+ cells by X4 (CXCR4-dependent) human immunodeficiency virus (HIV) strains. We previously showed that SDF-1α interacts specifically with heparin or heparan sulfates (HSs). Herein, we delimited the boundaries of the HS-binding domain located in the first β-strand of SDF-1α as the critical residues. We also provide evidence that binding to cell surface heparan sulfate proteoglycans (HSPGs) determines the capacity of SDF-1α to prevent the fusogenic activity of HIV-1 X4 isolates in leukocytes. Indeed, SDF-1α mutants lacking the capacity to interact with HSPGs showed a substantially reduced capacity to prevent... |
Tipo: Journal Article |
Palavras-chave: HIV; TEST PAR FLUORESCENCE; CYTOMÉTRIE DE FLUX; SDF-1; PROTEOGLYCAN HEPARAN SULFATE MEMBRANAIRE; CONSTANTE CINÉTIQUE; ANTICORPS MONOCLONAL; FLUORESCENCE RECEPTOR CXCR4; DENDRITIC CELLS; LYMPHOCYTE CHEMOATTRACTANT; GASTROINTESTINAL-TRACT; ENVELOPE GLYCOPROTEIN; EPITHELIAL-CELLS; RHESUS MACAQUES; FACTOR-I; T-CELLS; SDF-1. |
Ano: 2001 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200011223090281&uri=/notices/prodinra1/2010/10/ |
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Monnet, C.; Laune, D.; Laroche-Traineau, J.; Biard-Piechaczyk, M.; Briant, L.; Bès, C.; Pugnière, M.; Mani, J.C.; Pau, B.; Cerutti, M.; Devauchelle, G.; Devaux, C.; Granier, C.; Chardès, T.. |
Tipo: Journal Article |
Palavras-chave: HIV; ACTIVATION; CELLULE INFECTEE; ANTICORPS MONOCLONAL; TRANSCRIPTION; IMMUNODEFICIENCE VIRALE. |
Ano: 1999 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025186106078&uri=/notices/prodinra1/2010/09/ |
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Registros recuperados: 18 | |
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