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Cyclooxygenase-2 and hypoxia-regulated proteins are modulated by basic fibroblast growth factor in acute renal failure Biol. Res.
Villanueva,Sandra; Escobar,Pía; Jacubovsky,Ioram; Irarrázabal,Carlos; Carreño,Juan E; Erpel,José M; Céspedes,Carlos; González,Alexis A; Vio,Carlos P; Velarde,Victoria.
Acute renal failure (ARF) can be caused by injuries that induce tissue hypoxia, which in turn can trigger adaptive or inflammatory responses. We previously showed the participation of basic fibroblast growth factor (FGF-2) in renal repair. Based on this, the aim of this study was to analyze the effect of FGF-2 signaling pathway manipulation at hypoxia-induced protein levels, as well as in key proteins from the vasoactive systems of the kidney. We injected rat kidneys with FGF-2 recombinant protein (r-FGF) or FGF-2 receptor antisense oligonucleotide (FGFR2-ASO) after bilateral ischemia, and evaluated the presence of iNOS, EPO and HO-1, in representation of hypoxia-induced proteins, as well as COX-2, renin, kallikrein, and B2KR, in representation of the...
Tipo: Journal article Palavras-chave: ARF; COX-2; FGF-2; Kidney regeneration.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100007
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Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum Electron. J. Biotechnol.
Ding,Yu Fei; Wang,Chuan Tang; Tang,Yue Yi; Wang,Xiu Zhen; Wu,Qi; Hu,Dong Qing; Yu,Hong Tao; Zhang,Jian Cheng; Cui,Feng Gao; Song,Guo Sheng; Gao,Hua Yuan; Yu,Shan Lin.
Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively,...
Tipo: Journal article Palavras-chave: Arachis hypogaea; ARF; Cyclophilin; Differentially expressed genes; Genefishing; Ralstonia solanacearum.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500001
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