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Registros recuperados: 14
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Anti-predator response of Haliotis tuberculata is modified after only one generation of domestication ArchiMer
Roussel, Sabine; Bisch, T; Lachambre, S; Boudry, Pierre; Gervois, Jl; Lambert, Christophe; Huchette, S; Day, R.
Domestication of Haliotis tuberculata has only recently begun. During the process, we expect that behavioural and physiological traits may evolve to become more adapted to their captive environment. These modifications may result from intentional selection of production traits or unconscious and unintentional selection due to conditions experienced in the farm environment. To study this process at the earliest stage, the progeny of 3 different broodstocks obtained from wild parents, selected farmed abalone and randomly sampled farmed abalone, were studied. After rearing for 16 mo in separate tanks, offspring from the 3 progenies were placed together in sea cages at the same density. After 3 yr, behavioural traits were studied, and the immune status after a...
Tipo: Text Palavras-chave: Predation; Hiding behaviour; Haliotis tuberculata; Domestication; Selection; Captive; Abalone.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59841/62989.pdf
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Bilan des travaux de la cellule zoosanitaire de La Trinité Sur Mer. Réseau de Pathologie des Mollusques. Secteurs Nord-Loire. Année 1998 ArchiMer
Martin, Anne-genevieve; Tige, Gilbert; Hirata, Toshio; Kuntz, Gregoire; Le Coguic, Yvette.
Within the context of the mollusc pathology monitoring network, this report assesses the follow-up and work done by the zoosanitary unit of Trinité-sur-Mer on the whole of the French farming and bed areas of the North Loire for the year 1998.
Tipo: Text Palavras-chave: Herpes virus; Pathology; Mortality; Britanny (France); Normandy; Clam; Abalone; Cockle; Mussel; Pacific cupped oyster; European flat oyster; Herpès virus; Pathologie; Mortalité; Bretagne; Normandie; Naissain; Palourde; Ormeau; Coque; Moule; Huître creuse; Huître plate.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/rapport-1920.pdf
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Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene ArchiMer
Chen, M.h.; Kuo, S. T.; Renault, Tristan; Friedman, C. S.; Chang, P. H..
A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an...
Tipo: Text Palavras-chave: Herpes virus; Abalone; Haliotis diversicolor supertexta; DNA polymerase; Polymerase chain reaction.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20952/19675.pdf
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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Interaction de la bactérie Vibrio harveyi avec son hôte, l'hormeau Haliotis tuberculata : approches physiologiques, cellulaires et moléculaires ArchiMer
Travers, Marie-agnes.
Abalone aquaculture only recently developed in western Europe, but already it has to face numerous pathologies. Since 1998, the natural and farm stocks of Haliotis tuberculata suffer regularly high mortalities, mainly due to the pathogenic bacterium, Vibrio harveyi. The vibriosis of the European abalone, in particular its macroscopic signs, and the conditions necessary for its development have been studied in detail by field and laboratory experiments. We have been able to show the importance of the interplay between water temperature (>17°C), animal physiology (reproduction period) and a pathogenic strain (harbouring the plasmid pVCR1) for the development of this abalone disease. A limited localisation study of the abalone target tissues (gills, muscle...
Tipo: Text Palavras-chave: Ormeau; Haliotis tuberculata; Bactéries; Vibrio harveyi; Vibriose; Température; Immunité; Reproduction; Hémocytes; MAP Kinases; Plasmide; Virulence; GFP; Abalone; Haliotis tuberculata; Bacteria; Vibrio harveyi; Vibriosis; Temperature; Immunity; Reproduction; Haemocytes; MAP Kinases; Plasmid; Virulence; GFP.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00176/28729/27196.pdf
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Les mortalités d'ormeaux en Bretagne nord en 1998: synthèse des observations et analyses ArchiMer
Mazurie, Joseph; Thebault, Anne; Le Mao, Patrick; Veron, Gerard; Tige, Gilbert; Richard, Olivier.
Des mortalités anormales d'ormeaux ont été signalées en Bretagne nord (départements d'Ille et Vilaine et Côtes d'Armor) à partir du mois d'août 1998 et porté atteinte à la pêcherie professionnelle de cette zone. Des mortalités de même type avaient été signalées en Bretagne Sud, l'année précédente. Les mortalités de 1998 ont épargné le secteur plus à l'ouest (le Finistère) et plus à l'est (la Normandie). Une estimation précise n'a pas été entreprise, mais les pertes signalées par endroits dépassent 50% du stock. Les ormeaux atteints, de toutes tailles, ne présentent pas de symptôme particulier. Les analyses histologiques réalisées en priorité n'ont pas révélé d'agent infectieux. Toutefois, dans le doute et devant le caractère évolutif du phénomène, une...
Tipo: Text Palavras-chave: Ormeau; Mortalité; Côtes d'Armor; Ille-et-Vilaine; Abalone; Mortality.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00105/21666/19244.pdf
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Mise en place d'un plan de sélection génétique pour l'ormeau européen Haliotis tuberculata ArchiMer
Lachambre, Sébastien.
In the context of an increasing demand and a declining supply from fisheries, worldwide aquaculture production of abalone has greatly increased in the last decades. Selective breeding has been initiated to improve growth in major abalone species. In France, the abalone production was initiated 30 years ago and struggles to develop. This CIFRE PhD co-financed by France Haliotis, the only French hatchery in activity, was initiated to study early domestication process of farmed abalone population and to provide knowledge required to design a selective breeding program adapted to this company. Wild and farmed populations were compared, showing that, until 2013, farmed and wild abalone presented similar performances. However, lower physiological and behavioural...
Tipo: Text Palavras-chave: Sélection génétique; Ormeaux; Croissance; Physiologie; Comportement; Selective Breeding; Abalone; Growth; Physiology; Behaviour.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00441/55231/56711.pdf
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Pathology of cultured paua Haliotis iris infected with a novel haplosporidian parasite, with some observations on the course of disease ArchiMer
Diggles, Bk; Nichol, J; Hine, Pm; Wakefield, S; Cochennec, Nathalie; Roberts, Rd; Friedman, Cs.
Mortalities among juvenile paua Haliotis iris Martyn 1784 in a commercial culture facility were reported in April 2000. Histology of moribund paua showed heavy systemic infections of a uni- to multi-nucleate stage of a novel organism later confirmed by transmission electron microscopy (TEM) and molecular studies to be a haplosporidian. Multinucleate plasmodia up to 25 μm diameter with up to 17 nuclei were detectable in wet preparations of hemolymph from heavily infected paua. The presence of the haplosporidian in the affected facility was associated with mortalities of slow growing 'runt' paua during the summer months. Total mortalities in affected raceways 6 mo after mortalities began were between 82.5 and 90%. Heavily infected paua exhibited...
Tipo: Text Palavras-chave: Haplosporidia; Abalone; Aquaculture; Pathology; Disease; New Zealand.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-2783.pdf
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Réseau de Pathologie des Mollusques - Secteurs Nord-Loire - Année 1999. Bilan des travaux de la cellule zoosanitaire de La Trinité-sur-Mer ArchiMer
Martin, Anne-genevieve; Tige, Gilbert; Hirata, Toshio; Le Coguic, Yvette; Chollet, Bruno; Robert, Maeva; Thebault, Anne.
Within the context of the mollusc pathology monitoring network, this report assesses the follow-up and work done by the zoosanitary unit of Trinité-sur-Mer on the whole of the French farming and bed areas of the North Loire for the year 1999.
Tipo: Text Palavras-chave: Herpes virus; Pathology; Mortality; Britanny (France); Normandy; Clam; Abalone; Cockle; Mussel; Pacific cupped oyster; European flat oyster; Herpès virus; Pathologie; Mortalité; Bretagne; Normandie; Naissain; Palourde; Ormeau; Coque; Moule; Huître creuse; Huître plate.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-1921.pdf
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Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore ArchiMer
Gobet, Angelique; Mest, Laetitia; Perennou, Morgan; Dittami, Simon M.; Caralp, Claire; Coulombet, Celine; Huchette, Sylvain; Roussel, Sabine; Michel, Gurvan; Leblanc, Catherine.
Background: Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host. In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized. Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass. Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet. We investigated the effect of four...
Tipo: Text Palavras-chave: Microbe-host interactions; Digestive microbiota; Abalone; Macroalgae; Holobiont.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00489/60071/63368.pdf
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The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA ArchiMer
Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H..
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10...
Tipo: Text Palavras-chave: Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00166/27690/25882.pdf
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The growth of the abalone Haliotis Tuberculata L. in Bay of Brest ArchiMer
Cochard, Jean-claude.
The growth of H. tuberculata L. was studied by analysing the distribution of the growth checks on the shell of 640 individuals. The average lengths of the seven first rings including the first one appear to fit weIl to the Von Bertalanffy model. The value of the growth parameter K = 0,33 is rather similar to those cited in Litterature for the same species but the average attainable size L%= 97,5 seems to be low in this area.
Tipo: Text Palavras-chave: Growth rings; Growth; Abalone; Ormer; Haliotis tuberculata.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-6380.pdf
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Transcriptome based SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata ArchiMer
Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre.
Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by...
Tipo: Text Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf
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Viruses infecting marine molluscs ArchiMer
Arzul, Isabelle; Corbeil, Serge; Morga, Benjamin; Renault, Tristan.
Although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. The lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. Our current knowledge is mostly restricted to viruses affecting farmed species such as oysters Crassostrea gigas, abalone Haliotis diversicolor supertexta or the scallop Chlamys farreri. Molecular approaches which are needed to identify virus affiliation have been carried out for a small number of viruses, most of them belonging to the Herpesviridae and birnaviridae families. These last years, the use of New Generation Sequencing approach has allowed increasing...
Tipo: Text Palavras-chave: Molluscs; Viruses; Diseases; Herpesviridae; Bivalve; Abalone.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48206/48319.pdf
Registros recuperados: 14
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