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Registros recuperados: 11
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Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line BJMBR
Gimba,E.R.P.; Chemale,G.; Farias,S.S.; Zaha,A..
We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus granulosus; Actin; Promoters; Cestode; Gene expression; Deletions.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100004
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Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle Anais da ABC (AABC)
Silva,Ana Paula P.; Alves,Gutemberg G.; Araújo,Alexandre H.B.; Sola-Penna,Mauro.
In this work, we report evidences that the association of phosphofructokinase and F-actin can be affected by insulin stimulation in rabbit skeletal muscle homogenates and that this association can be a mechanism of phos-phofructokinase regulation. Through co-sedimentation techniques, we observed that on insulin-stimulated tissues, approximately 70% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 28% in control. This phenomenon is accompanied by a 100% increase in specific phosphofructokinase activity in stimulated homogenates. Purified F-actin causes an increase of 230% in phosphofructokinase activity and alters its kinetic parameters. The presence of F-actin increases the affinity of phosphofructokinase for fructose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphofructokinase; Hormone; Rabbit; Actin; Metabolism; Glycolysis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000300008
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Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses Genet. Mol. Biol.
Poletto,Andréia B.; Wasko,Adriane P.; Oliveira,Claudio; Azevedo,Alexandre; Carvalho,Robson F.; Silva,Maeli Dal Pai; Foresti,Fausto; Martins,Cesar.
Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actin; Expression pattern; Nile tilapia; Oreochromis niloticus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200031
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Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi IPB - Escola Superior Agrária
Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, Altino.
Actins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural proteins, found in all eukaryotes. So, actin gene sequences are used as tools in scientific research, for example, for phylogenetic analysis. Actin in Phytophthora infestans is encoded by at least two genes, in contrast to unicellular and filamentous fungi (Candida albicans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis and...
Tipo: Article Palavras-chave: Phytophthora cinnamomi; Ink disease; Cytoskeleton; Actin.
Ano: 2010 URL: http://hdl.handle.net/10198/3389
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Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi IPB - Escola Superior Agrária
Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, Altino.
Actins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural proteins, found in all eukaryotes. So, actin gene sequences are used as tools in scientific research, for example, for phylogenetic analysis. Actin in Phytophthora infestans is encoded by at least two genes, in contrast to unicellular and filamentous fungi (Candida albicans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis and...
Tipo: ConferenceObject Palavras-chave: Phytophthora cinnamomi; Ink disease; Cytoskeleton; Actin.
Ano: 2010 URL: http://hdl.handle.net/10198/4261
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Molecular and phenotypic characterisation of novel Phaeoacremonium species isolated from esca diseased grapevines Naturalis
Essakhi, S.; Mugnai, L.; Crous, P.W.; Groenewald, J.Z.; Surico, G..
Petri disease and esca are very destructive grapevine decline diseases that occur in most countries where grapevine (Vitis vinifera) is cultivated. Phaeoacremonium species are among the principal hyphomycetes associated with symptoms of the two diseases, producing a range of enzymes and phytotoxic metabolites. The present study compared the phylogeny of a global collection of 118 Phaeoacremonium isolates from grapevines, in order to gain a better understanding of their involvement in Petri disease and esca. Phylogenetic analyses of combined DNA sequence datasets of actin and β-tubulin genes revealed the presence of 13 species of Phaeoacremonium solated from esca diseased grapevines. Phaeoacremonium aleophilum was the most frequently isolated species with...
Tipo: Article / Letter to the editor Palavras-chave: Actin; Β-tubulin; Esca; Morphology; Phaeoacremonium; Phylogeny.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/532098
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Morphological plasticity in Cladosporium sphaerospermum Naturalis
Dugan, F.M.; Braun, U.; Groenewald, J.Z.; Crous, P.W..
A morphologically distinct isolate of Cladosporium sphaerospermum from a North American patent collection, referenced as Cladosporium lignicola in the patent, was examined. Generic affinity was confirmed by scanning electron microscopic examination of conidiogenous loci and conidial hila. Species identity as C. sphaerospermum was indicated by DNA sequence data derived from actin and translation elongation factor 1-α genes, and the internal transcribed spacer region. The isolate broadens the morphological limits of C. sphaerospermum by production of obclavate, occasionally transversely septate conidia with subrostrate conidiogenous apices (‘alternarioid’ conidia), and by production of conidia larger than those in prior standard descriptions. Type material...
Tipo: Article / Letter to the editor Palavras-chave: Actin; Cladosporium Davidiella; EF1-α; ITS; Morphology; Wood-inhabiting.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/531979
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Ocorrência e caracterização molecular de Cryptosporidium spp. em caprinos jovens do município de Quixadá-Ceará. Repositório Alice
BRITO, R. L. L. de..
Resumo: O presente estudo teve como objetivo avaliar a ocorrência da infecção e realizar a caracterização molecular de Cryptosporidium spp. em caprinos jovens do município de Quixadá, Ceará, Brasil. Participaram do estudo um total de 400 animais, com idade entre três e 360 dias, com e sem padrão racial definido, nos quais 154 eram machos e 246 fêmeas, procedentes de 25 estabelecimentos rurais distribuídos em três circuitos. Amostras de fezes foram obtidas diretamente da ampola retal dos cabritos e cadastradas de acordo com o aspecto e a coloração. Uma quantidade de 200 mg de cada amostra foi distribuída em microtubos de 2 mL e congeladas ?in natura? a -20 °C, até o momento das extrações de DNA genômico do parasito com auxílio de kit comercial. Todas as...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Actina; Cryptosporidium meleagridis; Cryptosporidium ubiquitum; Cryptosporidium xiaoi; HSP 70; Subunit 18S rRNA; Subunidade 18S rRNA; Criptosporidiose; Caprino; Cabrito; Parasito de animal; Diarréia; Infecção; Parasitologia; Cryptosporidium; Actin; Diarrhoea; Coccidia; Goats; Infection.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1007393
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One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France ArchiMer
Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence.
Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in...
Tipo: Text Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf
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The cytoskeleton of the electric tissue of Electrophorus electricus, L. Anais da ABC (AABC)
MERMELSTEIN,CLAUDIA DOS SANTOS; COSTA,MANOEL LUIS; MOURA NETO,VIVALDO.
The electric eel Electrophorus electricus is a fresh water teleost showing an electrogenic tissue that produces electric discharges. This electrogenic tissue is distributed in three well-defined electric organs which may be found symmetrically along both sides of the eel. These electric organs develop from muscle and exhibit several biochemical properties and morphological features of the muscle sarcolema. This review examines the contribution of the cytoskeletal meshwork to the maintenance of the polarized organization of the electrocyte, the cell that contains all electric properties of each electric organ. The cytoskeletal filaments display an important role in the establishment and maintenance of the highly specialized membrane model system of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrophorus electricus; Cytoskeleton; Desmin; Actin; Alpha-actinin; Vinculin.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300008
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Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa ArchiMer
Prado-alvarez, Maria; Couraleau, Yann; Chollet, Bruno; Tourbiez, Delphine; Arzul, Isabelle.
Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where...
Tipo: Text Palavras-chave: Bonamia exitiosa; Bonamia ostreae; Actin; Haplosporidia; Whole-Genome Amplification.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00277/38871/37410.pdf
Registros recuperados: 11
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