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A chemically defined medium for production of actinomycin D by Streptomyces parvulus BABT
Queiroz Sousa,Maria de Fátima Vieira de; Lopes,Carlos Edison; Pereira Júnior,Nei.
A chemically defined medium consisting of D(+)fructose, L(-)threonine, K2HPO4, MgSO4.7H2O, ZnSO4.7H2O, CaCl2.2H2O, FeSO4.7H2O and deionized water, was developed to maximize the synthesis of actinomycin D by the Streptomyces parvulus DAUFPE 3124 strain. This medium resulted in the maximum antibiotic concentration of 133mg/L while using the original medium the production of actinomycin D was poor not surpassing 43mg/L.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycin D; Production; Streptomyces. parvulus; Synthetic medium.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300002
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Effect of actinomycin D on simian rotavirus (SA11) replication in cell culture BJMBR
Stefanelli,C.C.; Castilho,J.G.; Botelho,M.V.J.; Linhares,R.E.C.; Nozawa,C.M..
Rotaviruses are the major cause of viral diarrhea in humans and animals. Actinomycin D (Act D) is an antibiotic that intercalates DNA and therefore inhibits DNA-dependent transcription. The current study was carried out to assess the influence of Act D on the replication of simian rotavirus (SA11) in cell culture. Virus-infected MA-104 cell cultures were studied in the presence of Act D at concentrations of 1.25 and 2.5 µg/ml. Treatment of rotavirus-infected cells with 2.5 µg/ml Act D 48 h post-infection reduced the cytoplasmic metachromasia after staining with acridine orange by 25%. Viral RNA labeled with ³H-uridine in the presence of the drug was separated by polyacrylamide gel electrophoresis. Viral RNA replication was not affected by Act D, but...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Rotavirus; Actinomycin D; Replication; Cell culture.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400006
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INHIBITION OF MAYARO VIRUS REPLICATION BY PROSTAGLANDIN A1 IN Aedes albopictus CELLS Rev. Microbiol.
Barbosa,Joel Antonio; Rebello,Moacyr Alcoforado.
Prostaglandin A1 (PGA1) inhibits Mayaro virus replication in Aedes albopictus cells at nontoxic doses to uninfected cells. At 10 µg/ml, PGA1 decreases virus production by 90%. The presence of PGA1 during virus adsorption, with no treatment after infection, reduces virus yield by 41%. Antiviral activity is observed even when treatment starts at one or two hours post-infection. However, in cells pre-treated with PGA1 during 24 hours, virus replication is not impaired. Thus, events ocurring during initial stages of infection and after virus adsorption and penetration must be the target of PGA1 action. SDS-PAGE analysis of 35S-methionine labelled proteins shows that PGA1 inhibits the synthesis of viral proteins and induces the synthesis of polypeptides with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mayaro virus; Prostaglandin; Aedes albopictus cells; Heat-shock proteins; Actinomycin D.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300013
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